Staphylococcus phage SMSAP5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Triavirus; unclassified Triavirus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1W607|I1W607_9CAUD Uncharacterized protein OS=Staphylococcus phage SMSAP5 OX=1168612 PE=4 SV=1
MM1 pKa = 6.5MPKK4 pKa = 9.87YY5 pKa = 10.17RR6 pKa = 11.84VWDD9 pKa = 4.11EE10 pKa = 3.77YY11 pKa = 10.49TGRR14 pKa = 11.84IHH16 pKa = 7.51DD17 pKa = 3.58VVGFDD22 pKa = 4.15FIEE25 pKa = 4.64NEE27 pKa = 3.94VHH29 pKa = 5.58YY30 pKa = 10.32EE31 pKa = 3.93NYY33 pKa = 10.84AEE35 pKa = 4.68AEE37 pKa = 4.19ALIHH41 pKa = 7.04ARR43 pKa = 11.84DD44 pKa = 3.87FKK46 pKa = 11.16DD47 pKa = 3.46VEE49 pKa = 4.49LMQSTGFKK57 pKa = 9.95DD58 pKa = 3.34VHH60 pKa = 6.76GVEE63 pKa = 4.57IYY65 pKa = 10.72EE66 pKa = 4.04GDD68 pKa = 3.57IVQDD72 pKa = 3.83CYY74 pKa = 11.44SRR76 pKa = 11.84EE77 pKa = 3.66VSFIEE82 pKa = 4.42FKK84 pKa = 10.91EE85 pKa = 4.2GAFYY89 pKa = 9.38ITFSNVTEE97 pKa = 4.33LLSEE101 pKa = 4.09NDD103 pKa = 4.4DD104 pKa = 3.73IIEE107 pKa = 3.89IVGNIFEE114 pKa = 4.74NEE116 pKa = 3.73MLLEE120 pKa = 4.17VMRR123 pKa = 5.34

Molecular weight: 14.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1W600|I1W600_9CAUD Putative DNA-binding protein OS=Staphylococcus phage SMSAP5 OX=1168612 PE=4 SV=1
MM1 pKa = 6.5MARR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 9.8VIRR9 pKa = 11.84VRR11 pKa = 11.84IKK13 pKa = 10.5GKK15 pKa = 10.6LMTLRR20 pKa = 11.84EE21 pKa = 4.12VSEE24 pKa = 4.8KK25 pKa = 10.69YY26 pKa = 10.32HH27 pKa = 6.64ISPEE31 pKa = 4.02LLRR34 pKa = 11.84YY35 pKa = 9.46RR36 pKa = 11.84YY37 pKa = 8.24KK38 pKa = 10.75HH39 pKa = 6.21KK40 pKa = 10.45MRR42 pKa = 11.84GDD44 pKa = 3.41EE45 pKa = 4.19LLCGRR50 pKa = 11.84KK51 pKa = 9.44DD52 pKa = 3.52SKK54 pKa = 11.56SKK56 pKa = 11.53DD57 pKa = 3.15EE58 pKa = 4.06VEE60 pKa = 4.23YY61 pKa = 10.11MKK63 pKa = 10.94SQIKK67 pKa = 10.52DD68 pKa = 3.37EE69 pKa = 4.26EE70 pKa = 4.34KK71 pKa = 10.23EE72 pKa = 3.86RR73 pKa = 11.84EE74 pKa = 4.18KK75 pKa = 10.64IRR77 pKa = 11.84KK78 pKa = 8.59KK79 pKa = 10.87AILNLYY85 pKa = 8.27QRR87 pKa = 11.84NVRR90 pKa = 11.84AEE92 pKa = 3.91YY93 pKa = 10.0EE94 pKa = 4.17EE95 pKa = 3.95EE96 pKa = 3.87RR97 pKa = 11.84KK98 pKa = 9.88RR99 pKa = 11.84RR100 pKa = 11.84LRR102 pKa = 11.84PWLYY106 pKa = 10.66DD107 pKa = 3.11GTPQKK112 pKa = 10.53HH113 pKa = 6.08SRR115 pKa = 11.84DD116 pKa = 4.06PYY118 pKa = 10.08WFDD121 pKa = 3.01VTYY124 pKa = 11.33NQMFKK129 pKa = 10.23KK130 pKa = 9.86WSEE133 pKa = 3.76AA134 pKa = 3.32

Molecular weight: 16.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

12279

100

2066

292.4

33.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.173 ± 0.706

0.464 ± 0.111

6.328 ± 0.303

7.769 ± 0.544

3.982 ± 0.276

6.173 ± 0.602

1.751 ± 0.135

6.874 ± 0.407

9.642 ± 0.594

8.201 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.631 ± 0.159

6.54 ± 0.326

2.802 ± 0.28

3.665 ± 0.181

4.162 ± 0.228

6.328 ± 0.395

5.701 ± 0.275

5.44 ± 0.212

1.222 ± 0.152

4.153 ± 0.405
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski