Prevotella sp. CAG:891

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2058 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7LET0|R7LET0_9BACT NAD dependent epimerase/reductase-related protein OS=Prevotella sp. CAG:891 OX=1262937 GN=BN805_01253 PE=4 SV=1
MM1 pKa = 7.73QSGNLLNINLAHH13 pKa = 5.2VTEE16 pKa = 5.06AAMCLNVEE24 pKa = 3.9LTDD27 pKa = 4.39RR28 pKa = 11.84FFADD32 pKa = 4.46LEE34 pKa = 4.16QEE36 pKa = 4.28EE37 pKa = 4.54ISGGEE42 pKa = 3.71VHH44 pKa = 6.58ATITVRR50 pKa = 11.84ASAGNIYY57 pKa = 9.96IVQIGLKK64 pKa = 10.6GEE66 pKa = 4.19VTVPCDD72 pKa = 3.08RR73 pKa = 11.84CLDD76 pKa = 4.2PLTLPIDD83 pKa = 3.57VTDD86 pKa = 4.34TIKK89 pKa = 10.76IKK91 pKa = 10.75DD92 pKa = 4.19SEE94 pKa = 4.49PEE96 pKa = 4.08DD97 pKa = 3.69NDD99 pKa = 3.49NLEE102 pKa = 3.75IRR104 pKa = 11.84YY105 pKa = 8.43LASSSLTYY113 pKa = 10.59DD114 pKa = 4.01LSWDD118 pKa = 3.54VYY120 pKa = 10.42EE121 pKa = 5.81IIATSLPMQRR131 pKa = 11.84THH133 pKa = 7.48AEE135 pKa = 4.08SEE137 pKa = 4.52CNQEE141 pKa = 3.93VVSYY145 pKa = 11.32IMGDD149 pKa = 3.69DD150 pKa = 3.67EE151 pKa = 5.01STNQSDD157 pKa = 4.05EE158 pKa = 4.67DD159 pKa = 4.25DD160 pKa = 3.57MM161 pKa = 7.67

Molecular weight: 17.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7LGQ7|R7LGQ7_9BACT Uncharacterized protein OS=Prevotella sp. CAG:891 OX=1262937 GN=BN805_01145 PE=4 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.24KK6 pKa = 9.93KK7 pKa = 9.88KK8 pKa = 7.12RR9 pKa = 11.84HH10 pKa = 5.52KK11 pKa = 10.32MSTHH15 pKa = 5.04KK16 pKa = 10.3RR17 pKa = 11.84KK18 pKa = 9.87KK19 pKa = 8.77RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2058

0

2058

703646

29

2581

341.9

38.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.104 ± 0.06

1.389 ± 0.023

5.43 ± 0.036

6.179 ± 0.054

4.491 ± 0.038

6.756 ± 0.051

2.245 ± 0.027

6.01 ± 0.055

5.985 ± 0.053

9.363 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.749 ± 0.026

4.906 ± 0.055

3.923 ± 0.035

3.91 ± 0.033

5.044 ± 0.049

5.752 ± 0.046

5.828 ± 0.044

6.729 ± 0.046

1.18 ± 0.023

4.024 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski