Pontibacillus yanchengensis Y32

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Pontibacillus; Pontibacillus yanchengensis

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3964 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0A2TAL2|A0A0A2TAL2_9BACI Uncharacterized protein OS=Pontibacillus yanchengensis Y32 OX=1385514 GN=N782_11545 PE=4 SV=1
MM1 pKa = 7.59ANILITFASMSGNTEE16 pKa = 3.58EE17 pKa = 4.95MADD20 pKa = 3.94LLKK23 pKa = 10.95DD24 pKa = 4.41DD25 pKa = 4.46IEE27 pKa = 4.56KK28 pKa = 10.55HH29 pKa = 4.2GHH31 pKa = 5.04EE32 pKa = 4.37VTLEE36 pKa = 3.91EE37 pKa = 4.56LDD39 pKa = 3.82EE40 pKa = 4.91LSAHH44 pKa = 6.76DD45 pKa = 3.95LVHH48 pKa = 7.0YY49 pKa = 10.12EE50 pKa = 4.38GILLGSYY57 pKa = 7.6TWNDD61 pKa = 2.38GDD63 pKa = 4.68LPYY66 pKa = 10.47EE67 pKa = 4.0VEE69 pKa = 5.18DD70 pKa = 3.55IYY72 pKa = 11.59EE73 pKa = 4.39EE74 pKa = 4.67IEE76 pKa = 4.49DD77 pKa = 4.19VDD79 pKa = 3.87LTGIKK84 pKa = 10.12VAVFGSGDD92 pKa = 3.43TNYY95 pKa = 10.33PLYY98 pKa = 10.77CEE100 pKa = 4.47AVHH103 pKa = 6.13TFEE106 pKa = 5.28KK107 pKa = 10.81QLTSSGATLVQDD119 pKa = 3.58GLKK122 pKa = 9.96IEE124 pKa = 4.37MAPDD128 pKa = 3.33SDD130 pKa = 4.2EE131 pKa = 5.55DD132 pKa = 3.99FDD134 pKa = 5.18NISNFGDD141 pKa = 3.38AFATFFNKK149 pKa = 9.84TPVV152 pKa = 2.95

Molecular weight:
16.88 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0A2TCG5|A0A0A2TCG5_9BACI Protein RecA OS=Pontibacillus yanchengensis Y32 OX=1385514 GN=recA PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.02KK14 pKa = 8.47VHH16 pKa = 5.57GFRR19 pKa = 11.84SRR21 pKa = 11.84MSDD24 pKa = 2.85ANGRR28 pKa = 11.84QVLKK32 pKa = 10.29RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.96GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight:
5.34 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3964

0

3964

1154180

37

1570

291.2

32.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.504 ± 0.038

0.633 ± 0.012

5.356 ± 0.035

7.903 ± 0.044

4.489 ± 0.031

6.722 ± 0.038

2.34 ± 0.021

7.389 ± 0.039

6.347 ± 0.032

9.435 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.932 ± 0.017

4.456 ± 0.029

3.655 ± 0.026

4.356 ± 0.035

3.873 ± 0.024

6.231 ± 0.029

5.575 ± 0.022

7.011 ± 0.032

1.084 ± 0.015

3.707 ± 0.026

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski