Avon-Heathcote Estuary associated circular virus 17

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IMJ1|A0A0C5IMJ1_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 17 OX=1618240 PE=3 SV=1
MM1 pKa = 8.0PEE3 pKa = 4.31TIRR6 pKa = 11.84NAVLTNFNDD15 pKa = 3.37PMIWEE20 pKa = 4.56DD21 pKa = 3.91LSPKK25 pKa = 10.0LRR27 pKa = 11.84YY28 pKa = 8.48FAYY31 pKa = 10.33GEE33 pKa = 4.33EE34 pKa = 4.33ICPEE38 pKa = 4.07TKK40 pKa = 9.7RR41 pKa = 11.84EE42 pKa = 3.95HH43 pKa = 5.78FQCFAQAWKK52 pKa = 10.24PMRR55 pKa = 11.84LTGWKK60 pKa = 9.99KK61 pKa = 10.46LFPTAHH67 pKa = 6.06IEE69 pKa = 3.77IMRR72 pKa = 11.84GNFRR76 pKa = 11.84EE77 pKa = 3.95NEE79 pKa = 4.21AYY81 pKa = 10.09CSKK84 pKa = 10.49EE85 pKa = 3.9GKK87 pKa = 8.08LTEE90 pKa = 4.32YY91 pKa = 11.06GEE93 pKa = 4.42KK94 pKa = 10.45PNEE97 pKa = 4.01NGKK100 pKa = 10.41KK101 pKa = 8.74STLINYY107 pKa = 7.67KK108 pKa = 10.32RR109 pKa = 11.84KK110 pKa = 9.16IDD112 pKa = 3.81EE113 pKa = 4.49GDD115 pKa = 3.64SVLGIAEE122 pKa = 4.18MEE124 pKa = 4.83DD125 pKa = 3.67YY126 pKa = 10.92FPTYY130 pKa = 10.36VMYY133 pKa = 10.78RR134 pKa = 11.84NGLHH138 pKa = 5.91EE139 pKa = 4.66YY140 pKa = 9.48KK141 pKa = 10.46RR142 pKa = 11.84HH143 pKa = 4.61VRR145 pKa = 11.84SKK147 pKa = 10.59KK148 pKa = 8.37VQNDD152 pKa = 2.85RR153 pKa = 11.84TMPEE157 pKa = 3.82VYY159 pKa = 10.52VRR161 pKa = 11.84IGPAGTGKK169 pKa = 7.87TRR171 pKa = 11.84WMDD174 pKa = 3.31EE175 pKa = 3.91KK176 pKa = 10.96FGLDD180 pKa = 3.24GWIQAPDD187 pKa = 3.5NTGKK191 pKa = 9.71WFDD194 pKa = 3.42GCDD197 pKa = 3.21RR198 pKa = 11.84DD199 pKa = 5.11VILFDD204 pKa = 4.94DD205 pKa = 4.48VEE207 pKa = 5.43AGQIPPLSSWKK218 pKa = 9.87RR219 pKa = 11.84LCDD222 pKa = 3.93RR223 pKa = 11.84YY224 pKa = 10.18PLQVPIKK231 pKa = 10.41GGFITWKK238 pKa = 9.23PKK240 pKa = 10.1TIVFTSNTHH249 pKa = 6.5PKK251 pKa = 9.86LWWPNLSEE259 pKa = 4.3FDD261 pKa = 3.06VGAIEE266 pKa = 4.64RR267 pKa = 11.84RR268 pKa = 11.84ITEE271 pKa = 3.98IVVVEE276 pKa = 4.1

Molecular weight: 32.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IMJ1|A0A0C5IMJ1_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 17 OX=1618240 PE=3 SV=1
MM1 pKa = 7.47RR2 pKa = 11.84KK3 pKa = 9.88KK4 pKa = 9.39MRR6 pKa = 11.84KK7 pKa = 6.61NVRR10 pKa = 11.84RR11 pKa = 11.84IVNAMAEE18 pKa = 4.56TKK20 pKa = 10.47VEE22 pKa = 3.8LRR24 pKa = 11.84QVVNNLGVKK33 pKa = 10.23HH34 pKa = 6.3NFVTNYY40 pKa = 8.71TSNLLYY46 pKa = 10.21TDD48 pKa = 3.18NGTRR52 pKa = 11.84GEE54 pKa = 4.37MVNDD58 pKa = 3.81TASVSTVGIRR68 pKa = 11.84NGRR71 pKa = 11.84KK72 pKa = 9.57LYY74 pKa = 10.89GKK76 pKa = 10.36GIAIRR81 pKa = 11.84MKK83 pKa = 10.8LEE85 pKa = 3.89NFQAQPHH92 pKa = 5.88LVYY95 pKa = 10.96KK96 pKa = 10.59LMIVRR101 pKa = 11.84HH102 pKa = 6.11KK103 pKa = 10.37YY104 pKa = 10.47DD105 pKa = 3.26NSITATNDD113 pKa = 3.32NLWEE117 pKa = 4.46GAHH120 pKa = 5.69TDD122 pKa = 4.11KK123 pKa = 10.91MLDD126 pKa = 3.87YY127 pKa = 10.57IDD129 pKa = 3.9TEE131 pKa = 3.88SWEE134 pKa = 4.43IKK136 pKa = 8.68WSKK139 pKa = 10.95SYY141 pKa = 8.4TLKK144 pKa = 10.38MPGYY148 pKa = 9.72GSGASTSTTGQIAGTAQVDD167 pKa = 3.58QGDD170 pKa = 4.24GQAHH174 pKa = 6.91IGRR177 pKa = 11.84PKK179 pKa = 10.56RR180 pKa = 11.84FIKK183 pKa = 10.29EE184 pKa = 3.71YY185 pKa = 10.21IPINRR190 pKa = 11.84EE191 pKa = 3.49VFYY194 pKa = 10.93HH195 pKa = 6.46KK196 pKa = 10.9VQGTSSPEE204 pKa = 3.65DD205 pKa = 3.0HH206 pKa = 6.99TYY208 pKa = 9.32PLNMRR213 pKa = 11.84WQLVGFCYY221 pKa = 10.01SAYY224 pKa = 9.02TASTNGDD231 pKa = 3.43DD232 pKa = 6.38LGFLDD237 pKa = 5.69CSTKK241 pKa = 10.41FYY243 pKa = 10.98TKK245 pKa = 10.83DD246 pKa = 3.0LL247 pKa = 4.13

Molecular weight: 28.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

523

247

276

261.5

30.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.589 ± 0.486

1.338 ± 0.381

5.736 ± 0.049

6.501 ± 1.769

3.824 ± 0.714

7.648 ± 0.324

2.294 ± 0.389

5.927 ± 0.479

8.031 ± 0.244

6.119 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.25 ± 0.284

5.736 ± 0.827

4.589 ± 1.558

2.868 ± 0.56

6.119 ± 0.325

4.589 ± 1.071

7.457 ± 1.046

5.927 ± 0.397

2.677 ± 0.763

4.78 ± 0.641
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski