Hubei sobemo-like virus 24

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEZ7|A0A1L3KEZ7_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 24 OX=1923210 PE=4 SV=1
MM1 pKa = 6.97TVEE4 pKa = 5.62DD5 pKa = 4.22FDD7 pKa = 4.55EE8 pKa = 4.88DD9 pKa = 5.14RR10 pKa = 11.84VDD12 pKa = 2.92SWAYY16 pKa = 10.36ADD18 pKa = 4.85QEE20 pKa = 3.91MDD22 pKa = 3.2YY23 pKa = 11.59DD24 pKa = 3.85RR25 pKa = 11.84VLDD28 pKa = 4.5FGDD31 pKa = 4.46DD32 pKa = 3.87SADD35 pKa = 3.25EE36 pKa = 4.55EE37 pKa = 4.77YY38 pKa = 10.99RR39 pKa = 11.84FDD41 pKa = 5.91DD42 pKa = 5.06RR43 pKa = 11.84DD44 pKa = 3.62DD45 pKa = 4.75DD46 pKa = 6.01RR47 pKa = 11.84DD48 pKa = 3.99DD49 pKa = 3.51RR50 pKa = 11.84WSDD53 pKa = 3.81DD54 pKa = 3.51EE55 pKa = 5.41LDD57 pKa = 3.91AYY59 pKa = 9.53EE60 pKa = 5.65DD61 pKa = 3.37KK62 pKa = 11.4YY63 pKa = 11.24EE64 pKa = 4.34RR65 pKa = 11.84NRR67 pKa = 11.84EE68 pKa = 3.78KK69 pKa = 10.49RR70 pKa = 11.84DD71 pKa = 3.4RR72 pKa = 11.84AEE74 pKa = 4.01EE75 pKa = 3.83RR76 pKa = 11.84WDD78 pKa = 3.65YY79 pKa = 11.82LNDD82 pKa = 3.88DD83 pKa = 3.26RR84 pKa = 11.84DD85 pKa = 3.96DD86 pKa = 3.95YY87 pKa = 11.8VRR89 pKa = 11.84EE90 pKa = 3.87ALGISRR96 pKa = 11.84MTPAQKK102 pKa = 10.44NRR104 pKa = 11.84LKK106 pKa = 10.95SLLDD110 pKa = 3.42RR111 pKa = 11.84PLVVKK116 pKa = 10.06GQAPGAPNVEE126 pKa = 4.38HH127 pKa = 7.07PPPTEE132 pKa = 3.8EE133 pKa = 4.69LDD135 pKa = 3.52LDD137 pKa = 3.98EE138 pKa = 5.0VVGNLVEE145 pKa = 4.37RR146 pKa = 11.84VEE148 pKa = 4.11HH149 pKa = 7.02LEE151 pKa = 4.61AEE153 pKa = 4.26LANLKK158 pKa = 10.56GKK160 pKa = 10.24LIPKK164 pKa = 9.88KK165 pKa = 10.23GAPPVKK171 pKa = 10.05KK172 pKa = 9.78PKK174 pKa = 10.22KK175 pKa = 9.33CVHH178 pKa = 6.15CSEE181 pKa = 4.29MLEE184 pKa = 4.43SIAAAAKK191 pKa = 9.77HH192 pKa = 5.82RR193 pKa = 11.84RR194 pKa = 11.84EE195 pKa = 4.13QHH197 pKa = 6.18PEE199 pKa = 3.8AYY201 pKa = 8.36WAARR205 pKa = 11.84NATEE209 pKa = 3.81NPGYY213 pKa = 10.24KK214 pKa = 10.29GEE216 pKa = 4.33SANGADD222 pKa = 3.4FKK224 pKa = 11.71VRR226 pKa = 11.84VKK228 pKa = 9.58TQAKK232 pKa = 8.58PAFFRR237 pKa = 11.84GGSRR241 pKa = 11.84KK242 pKa = 7.28TSKK245 pKa = 10.09PSSVSSSTTSGSSSGSPSPQEE266 pKa = 3.87TRR268 pKa = 11.84KK269 pKa = 8.52STNGQNQSDD278 pKa = 3.69DD279 pKa = 4.04RR280 pKa = 11.84FDD282 pKa = 4.29KK283 pKa = 10.7FLQAIIGLASEE294 pKa = 5.05MKK296 pKa = 10.45QKK298 pKa = 11.01

Molecular weight: 33.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEZ7|A0A1L3KEZ7_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 24 OX=1923210 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.39SLAIHH7 pKa = 6.01SAMHH11 pKa = 6.2KK12 pKa = 10.24GLRR15 pKa = 11.84TDD17 pKa = 3.73PPSDD21 pKa = 3.63VQEE24 pKa = 4.55DD25 pKa = 4.68EE26 pKa = 3.91ILEE29 pKa = 4.17WMARR33 pKa = 11.84IYY35 pKa = 9.94PRR37 pKa = 11.84WEE39 pKa = 3.8IPSDD43 pKa = 3.48FMQYY47 pKa = 7.3THH49 pKa = 5.89YY50 pKa = 10.81QRR52 pKa = 11.84VLRR55 pKa = 11.84GLDD58 pKa = 3.24WTSSPGYY65 pKa = 9.26PYY67 pKa = 11.51LLTSPTNSQLFQVVDD82 pKa = 4.11GEE84 pKa = 4.26PAEE87 pKa = 4.38APSRR91 pKa = 11.84AIWEE95 pKa = 4.28IVQKK99 pKa = 10.88RR100 pKa = 11.84LVEE103 pKa = 4.13RR104 pKa = 11.84DD105 pKa = 3.21ADD107 pKa = 4.39PIRR110 pKa = 11.84LFVKK114 pKa = 10.21PEE116 pKa = 3.47PHH118 pKa = 6.48KK119 pKa = 10.75LKK121 pKa = 10.53KK122 pKa = 9.84IEE124 pKa = 3.93EE125 pKa = 4.2GRR127 pKa = 11.84FRR129 pKa = 11.84LISSVSVVDD138 pKa = 4.52QIIDD142 pKa = 3.47HH143 pKa = 6.38MLHH146 pKa = 7.32DD147 pKa = 6.13DD148 pKa = 3.73YY149 pKa = 11.63DD150 pKa = 3.65QVMIEE155 pKa = 3.98NWATVPPKK163 pKa = 10.79AGWTPLLGGWKK174 pKa = 8.18TVPNFLRR181 pKa = 11.84PLAVDD186 pKa = 3.83KK187 pKa = 11.07KK188 pKa = 9.42AWDD191 pKa = 3.28WTVRR195 pKa = 11.84PWLLSLSLRR204 pKa = 11.84HH205 pKa = 7.14RR206 pKa = 11.84IRR208 pKa = 11.84QCSNMCPLWEE218 pKa = 5.65DD219 pKa = 3.08LAEE222 pKa = 4.16WRR224 pKa = 11.84WRR226 pKa = 11.84MLYY229 pKa = 10.69AEE231 pKa = 4.63AVFVTSGGILLKK243 pKa = 10.55QKK245 pKa = 10.44EE246 pKa = 4.15PGVRR250 pKa = 11.84KK251 pKa = 9.85SGCVTTLSDD260 pKa = 3.98NSLEE264 pKa = 3.76QDD266 pKa = 2.94ILHH269 pKa = 6.37RR270 pKa = 11.84RR271 pKa = 11.84ISLDD275 pKa = 2.98MWRR278 pKa = 11.84KK279 pKa = 9.62NPDD282 pKa = 3.05LHH284 pKa = 7.55IPPLWGSMGDD294 pKa = 3.52DD295 pKa = 4.52TIQEE299 pKa = 4.23EE300 pKa = 4.52PDD302 pKa = 3.45NLDD305 pKa = 3.14EE306 pKa = 4.17YY307 pKa = 11.3LGYY310 pKa = 10.7LSQYY314 pKa = 9.86CIVKK318 pKa = 9.92EE319 pKa = 4.16AVRR322 pKa = 11.84SPDD325 pKa = 3.14FAGFSFHH332 pKa = 6.92GRR334 pKa = 11.84DD335 pKa = 3.6VKK337 pKa = 10.62PLYY340 pKa = 9.96LGKK343 pKa = 9.65HH344 pKa = 6.22AYY346 pKa = 9.23QLLHH350 pKa = 6.64VDD352 pKa = 3.79PAVKK356 pKa = 10.5DD357 pKa = 3.99GVARR361 pKa = 11.84SYY363 pKa = 11.74ALLYY367 pKa = 10.37HH368 pKa = 7.11RR369 pKa = 11.84APAARR374 pKa = 11.84WISRR378 pKa = 11.84MCRR381 pKa = 11.84MLGDD385 pKa = 3.42VPTEE389 pKa = 3.78VRR391 pKa = 11.84LDD393 pKa = 3.7SIYY396 pKa = 11.08DD397 pKa = 3.6GEE399 pKa = 4.23

Molecular weight: 46.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

697

298

399

348.5

40.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.03 ± 1.075

1.004 ± 0.211

9.469 ± 1.444

7.317 ± 1.319

2.439 ± 0.156

5.308 ± 0.038

2.582 ± 0.574

3.73 ± 1.302

6.169 ± 1.196

8.752 ± 1.72

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.439 ± 0.483

2.582 ± 0.703

6.6 ± 0.568

3.443 ± 0.056

7.891 ± 0.316

7.317 ± 0.467

3.443 ± 0.056

6.169 ± 0.508

2.869 ± 0.969

3.443 ± 0.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski