Sewage-associated circular DNA virus-20

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UGX8|A0A0B4UGX8_9VIRU ATP-dependent helicase Rep OS=Sewage-associated circular DNA virus-20 OX=1592087 PE=3 SV=1
MM1 pKa = 7.36ARR3 pKa = 11.84SRR5 pKa = 11.84NYY7 pKa = 9.93VFTVNNYY14 pKa = 10.31DD15 pKa = 5.49DD16 pKa = 4.98EE17 pKa = 6.72DD18 pKa = 3.91EE19 pKa = 4.47HH20 pKa = 7.63QCWAMPWEE28 pKa = 4.42VKK30 pKa = 9.79DD31 pKa = 3.94CKK33 pKa = 10.45YY34 pKa = 10.53ICVGKK39 pKa = 10.38EE40 pKa = 3.35IGEE43 pKa = 4.38SGTPHH48 pKa = 5.82LQGYY52 pKa = 9.41ICFTQLKK59 pKa = 10.02SLNQLKK65 pKa = 10.63GFFPSAHH72 pKa = 6.48FEE74 pKa = 4.5TKK76 pKa = 10.35RR77 pKa = 11.84GTHH80 pKa = 4.91QQAADD85 pKa = 3.65YY86 pKa = 9.74CKK88 pKa = 10.45KK89 pKa = 10.82DD90 pKa = 2.87GDD92 pKa = 3.89FFEE95 pKa = 4.9WGTLPVDD102 pKa = 3.89DD103 pKa = 5.43CGSAGAAAMDD113 pKa = 4.15EE114 pKa = 4.52LMGHH118 pKa = 6.29TIEE121 pKa = 5.56CIRR124 pKa = 11.84NGDD127 pKa = 3.57YY128 pKa = 10.91KK129 pKa = 11.06GIPNAATHH137 pKa = 6.66FIKK140 pKa = 10.57AAEE143 pKa = 3.89YY144 pKa = 10.15RR145 pKa = 11.84VLKK148 pKa = 9.91EE149 pKa = 3.78QQQDD153 pKa = 3.25RR154 pKa = 11.84NLATLDD160 pKa = 3.7TLTHH164 pKa = 4.4QWRR167 pKa = 11.84WGKK170 pKa = 10.3AGCGKK175 pKa = 10.37SKK177 pKa = 9.85PARR180 pKa = 11.84DD181 pKa = 4.3ANPDD185 pKa = 3.51AYY187 pKa = 11.31LKK189 pKa = 9.85MCNKK193 pKa = 8.79WWDD196 pKa = 3.73GYY198 pKa = 9.11TGQEE202 pKa = 3.82VVIIEE207 pKa = 5.24DD208 pKa = 3.79FDD210 pKa = 4.9PDD212 pKa = 4.12HH213 pKa = 6.92KK214 pKa = 11.19CLVHH218 pKa = 6.82HH219 pKa = 6.39LKK221 pKa = 9.91IWADD225 pKa = 3.87RR226 pKa = 11.84YY227 pKa = 10.85AFPAEE232 pKa = 4.23VKK234 pKa = 10.3GGKK237 pKa = 8.98IDD239 pKa = 3.64IRR241 pKa = 11.84PKK243 pKa = 10.2TIIITSNYY251 pKa = 8.64HH252 pKa = 5.73PRR254 pKa = 11.84DD255 pKa = 3.34IFDD258 pKa = 4.36RR259 pKa = 11.84EE260 pKa = 3.97PDD262 pKa = 3.15LEE264 pKa = 6.56AIMRR268 pKa = 11.84RR269 pKa = 11.84FNVTHH274 pKa = 5.92VLGDD278 pKa = 3.79LGVDD282 pKa = 4.05FMQVDD287 pKa = 3.64NSS289 pKa = 3.62

Molecular weight: 33.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UGX8|A0A0B4UGX8_9VIRU ATP-dependent helicase Rep OS=Sewage-associated circular DNA virus-20 OX=1592087 PE=3 SV=1
MM1 pKa = 7.47RR2 pKa = 11.84RR3 pKa = 11.84KK4 pKa = 9.62SSKK7 pKa = 9.73ARR9 pKa = 11.84KK10 pKa = 7.96PLRR13 pKa = 11.84NNRR16 pKa = 11.84VKK18 pKa = 10.64RR19 pKa = 11.84AISRR23 pKa = 11.84KK24 pKa = 6.81SLRR27 pKa = 11.84RR28 pKa = 11.84QIATQIRR35 pKa = 11.84RR36 pKa = 11.84QAEE39 pKa = 4.06TKK41 pKa = 7.85EE42 pKa = 3.95QKK44 pKa = 8.46ITAEE48 pKa = 5.05RR49 pKa = 11.84YY50 pKa = 9.71LLWGPTAAAFNTRR63 pKa = 11.84NIFATSDD70 pKa = 3.01ILATISQGTGQGDD83 pKa = 3.76RR84 pKa = 11.84IGNKK88 pKa = 9.09VSLVNFFFSFIVWARR103 pKa = 11.84ATNLGGPSLPIDD115 pKa = 3.19LCMYY119 pKa = 9.62VFSDD123 pKa = 3.65KK124 pKa = 11.18LNPTSQLSLNIQNSIAGITQPYY146 pKa = 9.42FYY148 pKa = 10.08EE149 pKa = 4.75AGNATTGANLTITDD163 pKa = 3.17QLLRR167 pKa = 11.84VNGDD171 pKa = 2.8RR172 pKa = 11.84FTLHH176 pKa = 6.32KK177 pKa = 10.54KK178 pKa = 10.39KK179 pKa = 10.18IFKK182 pKa = 10.39ISTSTVANWSNNDD195 pKa = 3.09YY196 pKa = 10.51KK197 pKa = 11.05ASRR200 pKa = 11.84KK201 pKa = 9.66FKK203 pKa = 10.76INLAKK208 pKa = 10.66YY209 pKa = 8.59MPKK212 pKa = 10.16LVQWNDD218 pKa = 2.96AGVLTSRR225 pKa = 11.84QVWVMFLPVIANDD238 pKa = 3.54DD239 pKa = 3.82TSTVFANSDD248 pKa = 3.5LVGIGFDD255 pKa = 4.03YY256 pKa = 9.92QVKK259 pKa = 8.9WKK261 pKa = 11.0DD262 pKa = 3.19MM263 pKa = 3.78

Molecular weight: 29.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

552

263

289

276.0

31.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.971 ± 0.279

1.993 ± 1.141

7.246 ± 1.629

3.623 ± 1.487

4.891 ± 0.306

6.522 ± 0.848

2.355 ± 1.397

6.522 ± 0.497

7.428 ± 0.125

6.703 ± 0.907

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.174 ± 0.193

5.797 ± 1.279

3.623 ± 0.411

4.529 ± 0.293

5.978 ± 0.881

5.072 ± 1.791

6.522 ± 1.035

5.254 ± 0.318

2.536 ± 0.18

3.261 ± 0.424
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski