Escherichia phage Lambda_ev017

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lambdavirus; unclassified Lambdavirus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653FYA2|A0A653FYA2_9CAUD Partial tonB-like membrane protein encoded within prophage CP-933N OS=Escherichia phage Lambda_ev017 OX=2742949 PE=4 SV=1
MM1 pKa = 8.44VEE3 pKa = 4.19EE4 pKa = 4.3TGSCITEE11 pKa = 4.05SATTVSRR18 pKa = 11.84CDD20 pKa = 3.25VCNVSQIMAQDD31 pKa = 3.36VVMWQSGTQDD41 pKa = 3.11ILSEE45 pKa = 4.11

Molecular weight: 4.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653FUH0|A0A653FUH0_9CAUD Exc domain-containing protein OS=Escherichia phage Lambda_ev017 OX=2742949 PE=4 SV=1
MM1 pKa = 7.01KK2 pKa = 10.29QSNGLLSVFMRR13 pKa = 11.84CHH15 pKa = 7.11LINRR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 9.37QFRR24 pKa = 11.84LVYY27 pKa = 10.18DD28 pKa = 3.69AVVRR32 pKa = 11.84QALCDD37 pKa = 3.53AKK39 pKa = 10.87EE40 pKa = 3.67RR41 pKa = 4.01

Molecular weight: 4.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

14505

38

1160

198.7

22.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.81 ± 0.619

1.365 ± 0.146

5.874 ± 0.253

6.343 ± 0.361

3.64 ± 0.201

7.149 ± 0.286

1.724 ± 0.127

5.419 ± 0.285

5.364 ± 0.325

8.121 ± 0.367

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.916 ± 0.202

3.992 ± 0.274

3.812 ± 0.223

4.474 ± 0.338

6.012 ± 0.282

6.246 ± 0.226

5.984 ± 0.322

6.929 ± 0.283

1.758 ± 0.12

3.068 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski