Streptococcus sanguinis (strain SK36)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus; Streptococcus sanguinis

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3CPG9|A3CPG9_STRSV Transcriptional regulator XRE family putative OS=Streptococcus sanguinis (strain SK36) OX=388919 GN=SSA_1691 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.47KK3 pKa = 9.84NQTYY7 pKa = 10.18DD8 pKa = 3.22LKK10 pKa = 11.32DD11 pKa = 2.91IMEE14 pKa = 4.4AVKK17 pKa = 10.69SEE19 pKa = 4.0EE20 pKa = 4.56LDD22 pKa = 4.85DD23 pKa = 5.86DD24 pKa = 3.94FCLYY28 pKa = 10.88AKK30 pKa = 10.6EE31 pKa = 3.9NGEE34 pKa = 5.18LNFQDD39 pKa = 4.45SYY41 pKa = 12.13LLADD45 pKa = 3.95YY46 pKa = 9.07PQVVDD51 pKa = 4.44NRR53 pKa = 11.84DD54 pKa = 3.04VYY56 pKa = 10.35PRR58 pKa = 11.84QVKK61 pKa = 9.37EE62 pKa = 3.65QDD64 pKa = 3.45LEE66 pKa = 4.4LIYY69 pKa = 10.88YY70 pKa = 10.41GEE72 pKa = 4.79DD73 pKa = 3.34FADD76 pKa = 3.52VLLSVMEE83 pKa = 4.22QKK85 pKa = 11.17AEE87 pKa = 4.12VTDD90 pKa = 4.09QEE92 pKa = 4.58CLQALLYY99 pKa = 10.58YY100 pKa = 10.12YY101 pKa = 10.46EE102 pKa = 6.27HH103 pKa = 7.95DD104 pKa = 4.79DD105 pKa = 5.84FMDD108 pKa = 4.11FDD110 pKa = 5.19KK111 pKa = 11.1DD112 pKa = 3.68TVLL115 pKa = 4.72

Molecular weight: 13.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3CQR7|A3CQR7_STRSV Conserved uncharacterized protein OS=Streptococcus sanguinis (strain SK36) OX=388919 GN=SSA_2154 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.18IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84KK15 pKa = 8.26HH16 pKa = 4.89GFRR19 pKa = 11.84HH20 pKa = 6.46RR21 pKa = 11.84MSTKK25 pKa = 9.02NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.87GRR39 pKa = 11.84KK40 pKa = 8.75VLAAA44 pKa = 4.31

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2269

0

2269

704109

30

3047

310.3

34.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.978 ± 0.063

0.525 ± 0.014

5.665 ± 0.045

7.122 ± 0.056

4.563 ± 0.041

6.683 ± 0.057

1.812 ± 0.023

6.874 ± 0.046

6.636 ± 0.048

10.236 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.387 ± 0.024

4.225 ± 0.037

3.466 ± 0.035

4.357 ± 0.044

4.063 ± 0.04

6.407 ± 0.086

5.473 ± 0.062

6.831 ± 0.048

0.927 ± 0.017

3.77 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski