Bradyrhizobiaceae bacterium SG-6C

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; unclassified Bradyrhizobiaceae

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7QN18|F7QN18_9BRAD DD-transpeptidase OS=Bradyrhizobiaceae bacterium SG-6C OX=709797 GN=CSIRO_3170 PE=3 SV=1
MM1 pKa = 7.45SATAAAGVLSNDD13 pKa = 3.08TFGADD18 pKa = 3.3GASVVGVAKK27 pKa = 9.44GTTNANLDD35 pKa = 3.55NTGTLGAGLHH45 pKa = 5.32GTYY48 pKa = 8.88GTLTLNADD56 pKa = 3.45GSYY59 pKa = 10.82SYY61 pKa = 11.01IRR63 pKa = 11.84DD64 pKa = 3.61ANSAGGVTDD73 pKa = 3.91TFTYY77 pKa = 9.47TIKK80 pKa = 11.15DD81 pKa = 3.23GDD83 pKa = 4.1GDD85 pKa = 4.18LSHH88 pKa = 6.71TTLTITLPDD97 pKa = 3.59ATPSYY102 pKa = 9.62TLPTAGNGVTTVYY115 pKa = 10.43EE116 pKa = 3.86AGLAVRR122 pKa = 11.84SGEE125 pKa = 4.0PAGSHH130 pKa = 6.16IGATNITATGVIGVSSVDD148 pKa = 3.52GVGSVSLNGQTVTGTSAGTATTVLSNSTGTLTAWYY183 pKa = 7.85EE184 pKa = 3.87AATSQIHH191 pKa = 4.33YY192 pKa = 9.06TYY194 pKa = 10.71TLLDD198 pKa = 3.46NTLNTSGTSISVPVGVTDD216 pKa = 5.78LDD218 pKa = 3.99GDD220 pKa = 4.22TPASGGNLVITIVDD234 pKa = 3.92DD235 pKa = 4.4APVAVSDD242 pKa = 4.05TNSLGTASVGSQASGNLIAGTITSGSGTGGADD274 pKa = 3.23TQGADD279 pKa = 3.41GAHH282 pKa = 6.65IGSVTGGASDD292 pKa = 3.64GSGGFNVAGTYY303 pKa = 7.48GTLHH307 pKa = 6.21VDD309 pKa = 3.26GNGAYY314 pKa = 10.22SYY316 pKa = 10.46TRR318 pKa = 11.84TSAAGGTDD326 pKa = 3.23TFHH329 pKa = 6.37YY330 pKa = 9.68TLTDD334 pKa = 3.38GDD336 pKa = 4.4GDD338 pKa = 3.58ISNQADD344 pKa = 3.77LTITLDD350 pKa = 3.24DD351 pKa = 3.65TGRR354 pKa = 11.84LFVGSNDD361 pKa = 4.2SDD363 pKa = 4.96DD364 pKa = 4.47NPNDD368 pKa = 3.47PAHH371 pKa = 6.22FVDD374 pKa = 4.2VSSNVTGVINGGGGNDD390 pKa = 4.39TIAGDD395 pKa = 3.96PGATPTISAGSSANIVLVLDD415 pKa = 4.2TTASLTAQQMSAMQQAVGALLNSLYY440 pKa = 10.96NSGAANVRR448 pKa = 11.84VSMYY452 pKa = 10.83AFGGGYY458 pKa = 7.04TQLGTYY464 pKa = 10.27NIVANSHH471 pKa = 6.01SNAAADD477 pKa = 3.62AAALVSALDD486 pKa = 3.65AANADD491 pKa = 3.71GSITNSGSRR500 pKa = 11.84LYY502 pKa = 10.38IGADD506 pKa = 3.15PGSNTSYY513 pKa = 11.62ADD515 pKa = 3.47GLDD518 pKa = 3.4AVIDD522 pKa = 3.92YY523 pKa = 7.37LTPGTSGGDD532 pKa = 3.43PVSAVPLTGASVNKK546 pKa = 10.15VIFISDD552 pKa = 4.2GEE554 pKa = 4.36PTTNATQTQINTITGTGAGQYY575 pKa = 10.1GYY577 pKa = 9.0TIEE580 pKa = 4.87AVGLNVAGSPNAVAALNALDD600 pKa = 4.64SGHH603 pKa = 6.73SYY605 pKa = 10.79TNVVNPSDD613 pKa = 3.51LTGVVGSLAGSGVVQSAAGNDD634 pKa = 4.09VINGGAGKK642 pKa = 10.04DD643 pKa = 3.38IIYY646 pKa = 10.23GDD648 pKa = 3.77VMNTDD653 pKa = 3.76VLRR656 pKa = 11.84AAAGLSAATYY666 pKa = 8.45PAGSGWGVFAALEE679 pKa = 4.25GTTTAFTTANDD690 pKa = 3.96LAGDD694 pKa = 4.66GPQWTRR700 pKa = 11.84ADD702 pKa = 3.91TIAYY706 pKa = 8.43IAAHH710 pKa = 5.41QSEE713 pKa = 4.54LSKK716 pKa = 11.12EE717 pKa = 4.19SGRR720 pKa = 11.84TGGDD724 pKa = 3.23DD725 pKa = 3.89TITGGMGDD733 pKa = 5.02DD734 pKa = 4.52IIFAQEE740 pKa = 4.06GNDD743 pKa = 3.59KK744 pKa = 10.39IYY746 pKa = 10.67YY747 pKa = 9.13AQGDD751 pKa = 3.92GKK753 pKa = 9.96DD754 pKa = 3.7TVDD757 pKa = 4.03GGSGSDD763 pKa = 3.14TLYY766 pKa = 10.66ISGATGTVTIAAATVGPDD784 pKa = 3.07IVPATGTNYY793 pKa = 10.23TDD795 pKa = 4.48IIVTMSDD802 pKa = 2.92GGSIRR807 pKa = 11.84MDD809 pKa = 3.26SVEE812 pKa = 5.58DD813 pKa = 3.47IDD815 pKa = 3.86ITAGPGGVNIQYY827 pKa = 10.08IGSLASTALDD837 pKa = 3.46TSTVTVHH844 pKa = 6.73GGTGDD849 pKa = 3.83DD850 pKa = 4.92VLDD853 pKa = 3.54LTQRR857 pKa = 11.84GNTDD861 pKa = 3.12PHH863 pKa = 8.1KK864 pKa = 10.99VIADD868 pKa = 4.06GGAQTTADD876 pKa = 3.18IVKK879 pKa = 10.29LDD881 pKa = 3.81FSVSEE886 pKa = 3.88ITAIQAISGGVKK898 pKa = 8.25ITHH901 pKa = 6.87AGGAGTITDD910 pKa = 3.61EE911 pKa = 4.31FTNFEE916 pKa = 4.15SFQFEE921 pKa = 4.25GGVTKK926 pKa = 10.07TLAEE930 pKa = 4.21VQNLDD935 pKa = 5.19LIPPTLTSITMNDD948 pKa = 3.14TALKK952 pKa = 10.28VGEE955 pKa = 4.25TSLVTVVFSEE965 pKa = 4.24KK966 pKa = 10.68VNNLEE971 pKa = 4.16VTDD974 pKa = 4.6FTAPGGALSNLSSADD989 pKa = 3.56GGQTWTMTFIPTTNSTSSGNQISLANNSYY1018 pKa = 10.58TDD1020 pKa = 3.14IAGNTGSGGTSAATYY1035 pKa = 10.53SIDD1038 pKa = 3.42TKK1040 pKa = 11.35APTVTINGDD1049 pKa = 3.25VDD1051 pKa = 3.91PVTGQQVVFDD1061 pKa = 4.11VVFSEE1066 pKa = 4.57SVTGFTGADD1075 pKa = 2.82ISFTGSTAPGSLSASVTGSGTTYY1098 pKa = 10.3QVTVTGMTGSGSVVASVIAGAALDD1122 pKa = 3.89AAGNASAASTSTDD1135 pKa = 2.97NAVAFTLNLPSFIIAPSDD1153 pKa = 3.51LQYY1156 pKa = 11.56DD1157 pKa = 4.01PTSSSDD1163 pKa = 3.09VTAFNRR1169 pKa = 11.84IMFSDD1174 pKa = 4.54PDD1176 pKa = 3.81TAGSVTVHH1184 pKa = 6.15LTRR1187 pKa = 11.84TSGDD1191 pKa = 3.31GTLSATSTSDD1201 pKa = 3.37VVVSGSSSDD1210 pKa = 3.86MILTGTVDD1218 pKa = 4.93AINAYY1223 pKa = 10.12LAGNKK1228 pKa = 7.22VTLDD1232 pKa = 3.47TQGNDD1237 pKa = 2.78TDD1239 pKa = 4.09VFTISIDD1246 pKa = 3.73DD1247 pKa = 3.79GTTTISQTNYY1257 pKa = 8.56TVSDD1261 pKa = 3.63VSFTSSGSANTNNYY1275 pKa = 10.23AGVNVYY1281 pKa = 7.53EE1282 pKa = 4.29TSLDD1286 pKa = 3.66SGNGNDD1292 pKa = 3.83TLYY1295 pKa = 10.72TSWSHH1300 pKa = 6.38LGSSATAYY1308 pKa = 10.5AGGGGTGDD1316 pKa = 3.97TINLIFTADD1325 pKa = 3.45QLQEE1329 pKa = 4.05ILSSGTTRR1337 pKa = 11.84DD1338 pKa = 3.54DD1339 pKa = 3.62LQAFLAAPTGDD1350 pKa = 3.64TLSLGSTSWRR1360 pKa = 11.84ASASGFEE1367 pKa = 4.16TANIQLADD1375 pKa = 4.46LYY1377 pKa = 10.39ATRR1380 pKa = 11.84DD1381 pKa = 3.24TTTPSNNYY1389 pKa = 9.71FDD1391 pKa = 6.13INTTWKK1397 pKa = 10.65SVITSSEE1404 pKa = 3.91LLGTNGDD1411 pKa = 3.8GNNNFIVASAAGQSLSGNGGNDD1433 pKa = 2.91VMVALNGGNTLNGGAGLDD1451 pKa = 3.86LLLGGSGSDD1460 pKa = 3.37TLIGGDD1466 pKa = 4.04GIDD1469 pKa = 3.7RR1470 pKa = 11.84LSGGGGIDD1478 pKa = 2.92HH1479 pKa = 6.96FRR1481 pKa = 11.84FNATSEE1487 pKa = 4.23AGDD1490 pKa = 4.16HH1491 pKa = 6.91IMDD1494 pKa = 4.92FVHH1497 pKa = 6.52GTDD1500 pKa = 4.56TIDD1503 pKa = 3.7LLAAAFGGGSGAIVSADD1520 pKa = 4.44LIQITDD1526 pKa = 3.63AQNPSTIDD1534 pKa = 2.94MGSAHH1539 pKa = 7.15FAYY1542 pKa = 10.04QQSTGSLYY1550 pKa = 10.59YY1551 pKa = 10.61DD1552 pKa = 4.05PNGGAADD1559 pKa = 3.73ANRR1562 pKa = 11.84ILLAVLDD1569 pKa = 3.79NHH1571 pKa = 7.05AALTATDD1578 pKa = 3.41VHH1580 pKa = 6.87KK1581 pKa = 11.27VV1582 pKa = 3.16

Molecular weight: 157.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7QFR9|F7QFR9_9BRAD Uncharacterized protein OS=Bradyrhizobiaceae bacterium SG-6C OX=709797 GN=CSIRO_0292 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84LATAGGRR28 pKa = 11.84KK29 pKa = 8.95VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4236

0

4236

1249219

37

2736

294.9

31.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.254 ± 0.051

0.815 ± 0.012

5.598 ± 0.031

5.192 ± 0.031

3.836 ± 0.023

8.397 ± 0.052

1.961 ± 0.02

5.589 ± 0.029

4.125 ± 0.031

9.592 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.591 ± 0.018

2.906 ± 0.023

5.193 ± 0.03

3.131 ± 0.022

6.662 ± 0.034

5.676 ± 0.029

5.485 ± 0.03

7.465 ± 0.033

1.255 ± 0.014

2.278 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski