Staphylococcus phage TEM123

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Dubowvirus; unclassified Dubowvirus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1W643|I1W643_9CAUD Uncharacterized protein OS=Staphylococcus phage TEM123 OX=1168613 PE=4 SV=1
MM1 pKa = 7.38TPNLQLYY8 pKa = 8.19NKK10 pKa = 10.13AYY12 pKa = 7.67EE13 pKa = 4.13TLQGYY18 pKa = 7.3GFPVISHH25 pKa = 6.98KK26 pKa = 10.67EE27 pKa = 3.86MQQEE31 pKa = 3.54IPYY34 pKa = 8.78PFFVIKK40 pKa = 10.08MPEE43 pKa = 4.15SNRR46 pKa = 11.84SKK48 pKa = 10.44YY49 pKa = 9.35TFDD52 pKa = 4.18SYY54 pKa = 11.85SGDD57 pKa = 3.55TNLVIDD63 pKa = 4.3IWSVSDD69 pKa = 5.13DD70 pKa = 4.13LGHH73 pKa = 7.54HH74 pKa = 7.1DD75 pKa = 4.81GLVKK79 pKa = 10.54RR80 pKa = 11.84CIDD83 pKa = 3.9DD84 pKa = 3.54LTPSVKK90 pKa = 10.15TNDD93 pKa = 3.22YY94 pKa = 11.29DD95 pKa = 4.92FEE97 pKa = 5.84EE98 pKa = 5.91DD99 pKa = 3.81DD100 pKa = 3.91TNITQLVDD108 pKa = 3.19DD109 pKa = 4.48TTNQEE114 pKa = 4.31LLHH117 pKa = 6.13TSLTISYY124 pKa = 7.98KK125 pKa = 9.72TFF127 pKa = 3.25

Molecular weight: 14.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1W657|I1W657_9CAUD Crossover junction endodeoxyribonuclease RusA OS=Staphylococcus phage TEM123 OX=1168613 PE=4 SV=1
MM1 pKa = 7.29ARR3 pKa = 11.84KK4 pKa = 9.59ARR6 pKa = 11.84IVTINDD12 pKa = 2.81KK13 pKa = 10.44PYY15 pKa = 10.06RR16 pKa = 11.84FSKK19 pKa = 11.02FEE21 pKa = 3.76MEE23 pKa = 5.0LIEE26 pKa = 4.34SHH28 pKa = 6.88GITAGMVSKK37 pKa = 10.43RR38 pKa = 11.84VKK40 pKa = 10.67DD41 pKa = 3.3GWEE44 pKa = 3.56LHH46 pKa = 5.97EE47 pKa = 6.15AMDD50 pKa = 4.24APEE53 pKa = 4.22GTRR56 pKa = 11.84LSEE59 pKa = 3.86YY60 pKa = 10.23RR61 pKa = 11.84EE62 pKa = 3.95KK63 pKa = 10.39KK64 pKa = 8.51TIEE67 pKa = 3.99RR68 pKa = 11.84LEE70 pKa = 3.92QARR73 pKa = 11.84LEE75 pKa = 4.26RR76 pKa = 11.84KK77 pKa = 9.75LEE79 pKa = 4.03RR80 pKa = 11.84KK81 pKa = 9.41RR82 pKa = 11.84KK83 pKa = 9.67RR84 pKa = 11.84EE85 pKa = 3.71AEE87 pKa = 3.97LRR89 pKa = 11.84RR90 pKa = 11.84KK91 pKa = 9.76KK92 pKa = 10.2PHH94 pKa = 6.56LFNVPQKK101 pKa = 10.45HH102 pKa = 4.8PRR104 pKa = 11.84GRR106 pKa = 11.84YY107 pKa = 8.83ACYY110 pKa = 10.52LMEE113 pKa = 5.36NDD115 pKa = 4.09IFVKK119 pKa = 10.41VKK121 pKa = 10.3KK122 pKa = 10.51

Molecular weight: 14.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

11777

100

910

273.9

31.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.859 ± 0.428

0.543 ± 0.093

6.19 ± 0.382

7.583 ± 0.61

4.186 ± 0.264

5.613 ± 0.44

1.452 ± 0.122

7.515 ± 0.414

9.119 ± 0.542

7.226 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.16

7.566 ± 0.352

2.811 ± 0.256

4.008 ± 0.286

4.11 ± 0.255

5.672 ± 0.257

6.36 ± 0.276

6.436 ± 0.394

1.087 ± 0.199

4.246 ± 0.398
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski