Candidatus Walczuchella monophlebidarum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriales incertae sedis; Candidatus Walczuchella

Average proteome isoelectric point is 8.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 243 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A068DNU0|A0A068DNU0_9FLAO GTPase Obg OS=Candidatus Walczuchella monophlebidarum OX=1415657 GN=obg PE=3 SV=1
MM1 pKa = 7.19YY2 pKa = 7.77WTLEE6 pKa = 3.98LASYY10 pKa = 10.9LEE12 pKa = 4.44DD13 pKa = 4.82APWPANKK20 pKa = 10.23DD21 pKa = 3.59EE22 pKa = 5.39LIDD25 pKa = 3.49YY26 pKa = 10.37AMRR29 pKa = 11.84SGAPLAVIEE38 pKa = 4.53NLQQLEE44 pKa = 4.38DD45 pKa = 4.67DD46 pKa = 4.01GDD48 pKa = 3.96TYY50 pKa = 11.66EE51 pKa = 4.9SIEE54 pKa = 4.85EE55 pKa = 3.8IWIDD59 pKa = 3.65YY60 pKa = 7.36PTNEE64 pKa = 4.44DD65 pKa = 3.74LFFNEE70 pKa = 5.67DD71 pKa = 3.37EE72 pKa = 4.76FSGKK76 pKa = 9.2NPKK79 pKa = 10.05KK80 pKa = 10.21FLRR83 pKa = 11.84KK84 pKa = 9.49

Molecular weight:
9.9 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A068DQB7|A0A068DQB7_9FLAO DNA-directed RNA polymerase subunit omega OS=Candidatus Walczuchella monophlebidarum OX=1415657 GN=FNIIJ_133 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.41RR3 pKa = 11.84TYY5 pKa = 10.21QPSGRR10 pKa = 11.84KK11 pKa = 8.78RR12 pKa = 11.84RR13 pKa = 11.84NVHH16 pKa = 5.8GFIKK20 pKa = 10.61RR21 pKa = 11.84MGTKK25 pKa = 9.99GGRR28 pKa = 11.84KK29 pKa = 8.08ILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.08GRR39 pKa = 11.84KK40 pKa = 8.37NLTVSNVRR48 pKa = 11.84YY49 pKa = 9.88KK50 pKa = 10.75RR51 pKa = 3.46

Molecular weight:
6.09 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

243

0

243

82362

39

1417

338.9

38.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.6 ± 0.104

1.121 ± 0.047

4.429 ± 0.081

6.664 ± 0.144

4.866 ± 0.139

6.125 ± 0.128

2.249 ± 0.06

10.177 ± 0.146

9.462 ± 0.155

9.416 ± 0.126

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.127 ± 0.047

5.439 ± 0.089

3.465 ± 0.066

3.363 ± 0.061

4.701 ± 0.09

6.893 ± 0.097

4.654 ± 0.078

5.282 ± 0.111

0.93 ± 0.065

4.036 ± 0.092

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski