Vibrio phage PWH3a-P1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 209 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4R277|M4R277_9CAUD Uncharacterized protein OS=Vibrio phage PWH3a-P1 OX=754058 GN=VPIG_00034 PE=4 SV=1
MM1 pKa = 7.57IKK3 pKa = 10.51NIEE6 pKa = 4.07FTHH9 pKa = 5.02NHH11 pKa = 5.71NGEE14 pKa = 4.36TYY16 pKa = 8.56TVLVEE21 pKa = 4.58YY22 pKa = 10.4IDD24 pKa = 4.34AAPLTFDD31 pKa = 4.57SNQASCPTDD40 pKa = 3.66LYY42 pKa = 11.27GDD44 pKa = 3.95RR45 pKa = 11.84EE46 pKa = 4.12ILDD49 pKa = 3.54VVVIDD54 pKa = 4.06AQGGEE59 pKa = 4.0VDD61 pKa = 4.08SRR63 pKa = 11.84FITDD67 pKa = 2.93EE68 pKa = 4.34MIWFEE73 pKa = 5.07IDD75 pKa = 3.23VCKK78 pKa = 10.33NTVDD82 pKa = 4.47DD83 pKa = 4.56VKK85 pKa = 11.39LDD87 pKa = 3.78TTGLTFMDD95 pKa = 4.63LWNEE99 pKa = 3.92GEE101 pKa = 4.54DD102 pKa = 4.11YY103 pKa = 11.33YY104 pKa = 11.44EE105 pKa = 4.1EE106 pKa = 4.22

Molecular weight: 12.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4QQ91|M4QQ91_9CAUD Uncharacterized protein OS=Vibrio phage PWH3a-P1 OX=754058 GN=VPIG_00098 PE=4 SV=1
MM1 pKa = 7.03FQTMQVIKK9 pKa = 8.03TQRR12 pKa = 11.84TFCKK16 pKa = 9.83PSEE19 pKa = 4.21VDD21 pKa = 4.22QEE23 pKa = 4.37WQTKK27 pKa = 7.26QQRR30 pKa = 11.84KK31 pKa = 9.56DD32 pKa = 3.26KK33 pKa = 10.68RR34 pKa = 11.84NKK36 pKa = 9.09PNRR39 pKa = 11.84KK40 pKa = 7.18TGRR43 pKa = 11.84NNKK46 pKa = 9.58RR47 pKa = 11.84NWEE50 pKa = 4.06MM51 pKa = 3.69

Molecular weight: 6.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

209

0

209

37187

50

855

177.9

20.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.284 ± 0.217

1.89 ± 0.137

6.83 ± 0.142

7.753 ± 0.18

4.437 ± 0.101

5.935 ± 0.145

1.923 ± 0.08

6.244 ± 0.15

8.344 ± 0.249

8.385 ± 0.15

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.439 ± 0.086

5.776 ± 0.138

2.842 ± 0.1

3.375 ± 0.101

3.98 ± 0.138

6.688 ± 0.176

5.924 ± 0.189

6.629 ± 0.205

1.557 ± 0.08

4.765 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski