Lentibacter virus vB_LenP_ICBM3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Zobellviridae; Cobavirinae; Siovirus; unclassified Siovirus

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2YRD4|A0A3G2YRD4_9CAUD Uncharacterized protein OS=Lentibacter virus vB_LenP_ICBM3 OX=2301530 PE=4 SV=1
MM1 pKa = 7.61AKK3 pKa = 10.08KK4 pKa = 8.7PTISTLQSGFNSTEE18 pKa = 3.89TLNSNFEE25 pKa = 4.21KK26 pKa = 10.87LRR28 pKa = 11.84DD29 pKa = 3.81ALDD32 pKa = 3.53NTLSLDD38 pKa = 3.43GSTPNAMEE46 pKa = 5.54ADD48 pKa = 3.65LDD50 pKa = 4.43LNGNNIIGATGLLINGTDD68 pKa = 3.58YY69 pKa = 11.52LIDD72 pKa = 3.63VEE74 pKa = 4.32AAKK77 pKa = 10.41AAALVAQAAAEE88 pKa = 3.93LAEE91 pKa = 4.56NNAEE95 pKa = 4.13TAEE98 pKa = 4.25VNAEE102 pKa = 3.92ASEE105 pKa = 4.25TAAGLLATAASTSATNAATSATAASVSEE133 pKa = 4.08TAAALSEE140 pKa = 4.43TNAATSEE147 pKa = 4.57TNASTSEE154 pKa = 4.06TNAAASALAASTSEE168 pKa = 4.09TNAASSEE175 pKa = 4.29TNAAASEE182 pKa = 4.43TAASTSEE189 pKa = 4.04TNAAASEE196 pKa = 4.43TAASTSEE203 pKa = 4.13TNAATSEE210 pKa = 4.33TNAATSEE217 pKa = 4.26TNAAASEE224 pKa = 4.21AASSASAAAALTSEE238 pKa = 4.28TAAALSEE245 pKa = 4.38ANASASEE252 pKa = 3.94AAAGLSEE259 pKa = 4.04TAAAASEE266 pKa = 4.09TAAASSASSASTSASNAASSASSAQASKK294 pKa = 11.0DD295 pKa = 3.4AALAALDD302 pKa = 4.23SFDD305 pKa = 5.84DD306 pKa = 4.2RR307 pKa = 11.84YY308 pKa = 11.02LGQKK312 pKa = 10.02VSDD315 pKa = 3.76PTLDD319 pKa = 3.44NDD321 pKa = 4.31GDD323 pKa = 4.21ALVAGALYY331 pKa = 10.66FNTTDD336 pKa = 5.31DD337 pKa = 3.03IMKK340 pKa = 9.94VYY342 pKa = 10.29EE343 pKa = 4.1GSVWVAAYY351 pKa = 10.26ASLSGALLANNNLSDD366 pKa = 4.16LANVSATRR374 pKa = 11.84TNLGLGTAATTASTDD389 pKa = 3.38YY390 pKa = 9.88ATAAQGSLADD400 pKa = 4.33SAIQPADD407 pKa = 3.51LATVATTGVYY417 pKa = 10.7ADD419 pKa = 4.07LTGKK423 pKa = 7.69PTLGTAAATDD433 pKa = 3.58STAYY437 pKa = 9.06ATAAQGALADD447 pKa = 4.12SAIQAADD454 pKa = 3.64LATVATTGAYY464 pKa = 10.05SDD466 pKa = 4.02LTGTPAPYY474 pKa = 10.84ADD476 pKa = 4.31ADD478 pKa = 3.77VDD480 pKa = 3.77THH482 pKa = 6.98LNTGTAATGEE492 pKa = 4.19YY493 pKa = 10.36LSWNGADD500 pKa = 3.98YY501 pKa = 11.23DD502 pKa = 4.07WATVPAGYY510 pKa = 10.87ADD512 pKa = 4.3ADD514 pKa = 3.69VDD516 pKa = 3.79THH518 pKa = 6.99LNTGTATTDD527 pKa = 3.59QVLAWTGSDD536 pKa = 3.88YY537 pKa = 11.79DD538 pKa = 3.85WVDD541 pKa = 3.43ASAGATGGGTDD552 pKa = 4.65KK553 pKa = 11.05IFVEE557 pKa = 4.27NGQVVTTNYY566 pKa = 9.03TVAATTNAMTTGPIDD581 pKa = 3.43INSGVTVTVEE591 pKa = 3.66TGGRR595 pKa = 11.84WVVII599 pKa = 4.05

Molecular weight: 58.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2YRN2|A0A3G2YRN2_9CAUD Uncharacterized protein OS=Lentibacter virus vB_LenP_ICBM3 OX=2301530 PE=4 SV=1
MM1 pKa = 7.87AIFTAIGTAVLGAAAATAGAAATAIVGGVVAAGAVAGIAGTVKK44 pKa = 10.41AGKK47 pKa = 8.56ATRR50 pKa = 11.84KK51 pKa = 9.44AAAAQQKK58 pKa = 9.65AQEE61 pKa = 4.31LQSKK65 pKa = 7.44RR66 pKa = 11.84QRR68 pKa = 11.84RR69 pKa = 11.84AAIRR73 pKa = 11.84SNILASARR81 pKa = 11.84ARR83 pKa = 11.84ASAQAAGTAQSSGLSGAIGAGRR105 pKa = 11.84SQLGAEE111 pKa = 4.56LGFGTQMSGLSANISKK127 pKa = 10.64FNMQAQTYY135 pKa = 10.35GDD137 pKa = 3.43IAKK140 pKa = 10.39LGFAAAGNPTAIADD154 pKa = 5.08PIAGVYY160 pKa = 10.19DD161 pKa = 3.96YY162 pKa = 11.44LKK164 pKa = 10.75KK165 pKa = 10.9

Molecular weight: 16.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12523

46

1487

223.6

24.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.902 ± 0.952

0.711 ± 0.161

6.484 ± 0.232

6.843 ± 0.313

3.673 ± 0.269

7.858 ± 0.738

1.493 ± 0.159

4.839 ± 0.28

5.989 ± 0.47

8.049 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.611 ± 0.187

4.576 ± 0.274

3.426 ± 0.205

3.569 ± 0.21

4.584 ± 0.306

6.851 ± 0.373

6.923 ± 0.483

6.636 ± 0.277

1.437 ± 0.167

3.545 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski