Rhizobium phage RHEph01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Paadamvirus; Rhizobium virus RHEph01

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7TMN9|L7TMN9_9CAUD Uncharacterized protein OS=Rhizobium phage RHEph01 OX=1220601 GN=RHEph01_gp054 PE=4 SV=1
MM1 pKa = 7.42IFKK4 pKa = 10.65DD5 pKa = 3.97GALLPAVPEE14 pKa = 4.35VEE16 pKa = 4.31TLPNLSPRR24 pKa = 11.84QLWLAALEE32 pKa = 4.26INTTKK37 pKa = 10.41AQVMAQIGTITDD49 pKa = 3.21AKK51 pKa = 10.65LRR53 pKa = 11.84ATLEE57 pKa = 4.1IEE59 pKa = 4.3LTEE62 pKa = 4.55PPPDD66 pKa = 3.89GYY68 pKa = 11.55VRR70 pKa = 11.84DD71 pKa = 3.84SFAVEE76 pKa = 4.07RR77 pKa = 11.84LRR79 pKa = 11.84DD80 pKa = 3.61MMGIPVDD87 pKa = 3.55QFDD90 pKa = 5.77ALWLWAGTLL99 pKa = 3.62

Molecular weight: 11.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7TKA4|L7TKA4_9CAUD Uncharacterized protein OS=Rhizobium phage RHEph01 OX=1220601 GN=RHEph01_gp019 PE=4 SV=1
MM1 pKa = 5.88THH3 pKa = 6.09SRR5 pKa = 11.84IGKK8 pKa = 7.75VTFKK12 pKa = 10.15TPRR15 pKa = 11.84SGGFGKK21 pKa = 9.25TMVPARR27 pKa = 11.84SGRR30 pKa = 11.84GYY32 pKa = 8.39VHH34 pKa = 6.72QYY36 pKa = 8.97FKK38 pKa = 10.78RR39 pKa = 11.84VSEE42 pKa = 4.48LEE44 pKa = 4.08WVPITLLEE52 pKa = 4.25YY53 pKa = 10.79VRR55 pKa = 11.84STRR58 pKa = 3.87

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13116

44

1257

238.5

26.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.628 ± 0.471

0.625 ± 0.109

5.901 ± 0.274

6.153 ± 0.351

4.003 ± 0.185

7.335 ± 0.302

1.998 ± 0.195

5.177 ± 0.209

5.817 ± 0.322

8.219 ± 0.359

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.206

3.789 ± 0.262

4.43 ± 0.283

3.919 ± 0.28

6.061 ± 0.23

6.351 ± 0.37

6.16 ± 0.317

6.702 ± 0.311

1.525 ± 0.174

2.737 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski