Flavobacterium enshiense DK69

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; Flavobacterium enshiense

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2848 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V6S848|V6S848_9FLAO Glyoxalase OS=Flavobacterium enshiense DK69 OX=1107311 GN=Q767_13730 PE=4 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84SKK4 pKa = 11.17LLLFYY9 pKa = 10.91GVLFLLFSGNLFGQDD24 pKa = 2.68IALSEE29 pKa = 4.27QFFGRR34 pKa = 11.84YY35 pKa = 9.91DD36 pKa = 3.26FTFFGNTLNPDD47 pKa = 4.03EE48 pKa = 4.48NTFMYY53 pKa = 9.72PDD55 pKa = 4.95SILTSSSADD64 pKa = 3.43LNLSSGSEE72 pKa = 3.73IEE74 pKa = 4.16KK75 pKa = 10.61AYY77 pKa = 10.58LYY79 pKa = 9.65WAGCGPGDD87 pKa = 5.32FNIKK91 pKa = 10.14LNNIDD96 pKa = 3.05IAAQRR101 pKa = 11.84TFSTIQASSGKK112 pKa = 9.69IFFAAFYY119 pKa = 9.8DD120 pKa = 3.34ITDD123 pKa = 3.52IVISQGNTTYY133 pKa = 10.2TVSEE137 pKa = 4.73LDD139 pKa = 3.76VNDD142 pKa = 4.66QIPEE146 pKa = 3.86YY147 pKa = 9.05WDD149 pKa = 3.53NGTHH153 pKa = 5.55FAGWAVLVVYY163 pKa = 10.58KK164 pKa = 11.04NNNLPLNVVNVYY176 pKa = 10.81DD177 pKa = 4.06GLEE180 pKa = 4.17SLSTTPSGGVGEE192 pKa = 4.5LNITIDD198 pKa = 3.92NLNVIDD204 pKa = 5.44DD205 pKa = 4.12VGSKK209 pKa = 9.89IGFIAWEE216 pKa = 4.25GDD218 pKa = 3.04RR219 pKa = 11.84GIPAPGTDD227 pKa = 3.62IIEE230 pKa = 4.3SLSINGITVSNPPLNPANNAFNGTNSVTGAQNLFNMDD267 pKa = 3.56LDD269 pKa = 3.91IYY271 pKa = 10.58NIQNFIDD278 pKa = 4.12IGDD281 pKa = 3.81EE282 pKa = 3.97SATIQLATGQDD293 pKa = 3.37YY294 pKa = 11.87VMINTIVTKK303 pKa = 10.73LNSQLPDD310 pKa = 3.05ATVTIDD316 pKa = 3.77QAEE319 pKa = 4.7GEE321 pKa = 4.56CNSRR325 pKa = 11.84NIAVNYY331 pKa = 9.76SVSNINATDD340 pKa = 4.15FLPAGTPISIYY351 pKa = 10.8AGGILIGTNQTQNDD365 pKa = 3.59IQIGDD370 pKa = 3.96SEE372 pKa = 4.38NFYY375 pKa = 11.22QFVTIPNGVPVNFSLEE391 pKa = 4.25IIVDD395 pKa = 3.6QPGVVTEE402 pKa = 4.16LQEE405 pKa = 4.36NNNNTTMDD413 pKa = 3.49VTLATGPTPNLLADD427 pKa = 5.63LISCNRR433 pKa = 11.84GYY435 pKa = 9.47TEE437 pKa = 4.17GLFDD441 pKa = 4.68FSAYY445 pKa = 10.02EE446 pKa = 3.93NTVTSDD452 pKa = 3.45PNSTVTFHH460 pKa = 7.46EE461 pKa = 4.5SQLEE465 pKa = 4.1ANAGINPIPDD475 pKa = 3.82PSNYY479 pKa = 9.96LATATPKK486 pKa = 10.25TIFVRR491 pKa = 11.84IDD493 pKa = 3.21GPEE496 pKa = 4.09CFTTTSFQLMVKK508 pKa = 8.71PCPPIVYY515 pKa = 9.93NAVSVNGDD523 pKa = 3.19GMNDD527 pKa = 3.34TFHH530 pKa = 8.08IEE532 pKa = 3.75GLRR535 pKa = 11.84DD536 pKa = 3.21VFVNHH541 pKa = 7.26EE542 pKa = 3.74IFIYY546 pKa = 10.18NRR548 pKa = 11.84WGRR551 pKa = 11.84EE552 pKa = 3.54VWKK555 pKa = 10.66GNNYY559 pKa = 7.89TPEE562 pKa = 3.58WDD564 pKa = 4.21GYY566 pKa = 10.16IKK568 pKa = 10.9DD569 pKa = 4.09GVGSNHH575 pKa = 6.56APDD578 pKa = 3.4GTYY581 pKa = 10.27FYY583 pKa = 10.69ILYY586 pKa = 10.33LNDD589 pKa = 3.66PNYY592 pKa = 9.49PKK594 pKa = 10.46PLNGYY599 pKa = 10.01LYY601 pKa = 10.62LNHH604 pKa = 7.19

Molecular weight: 66.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V6S8U0|V6S8U0_9FLAO Uncharacterized protein OS=Flavobacterium enshiense DK69 OX=1107311 GN=Q767_07315 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.91HH17 pKa = 4.39GFMDD21 pKa = 4.5RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.41KK42 pKa = 10.14LTVSSEE48 pKa = 3.92PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2848

0

2848

932535

46

2947

327.4

36.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.527 ± 0.06

0.836 ± 0.016

5.307 ± 0.035

6.499 ± 0.06

5.361 ± 0.044

6.518 ± 0.057

1.744 ± 0.021

7.756 ± 0.051

7.871 ± 0.074

8.968 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.358 ± 0.025

6.149 ± 0.049

3.5 ± 0.03

3.349 ± 0.027

3.242 ± 0.029

6.463 ± 0.043

6.012 ± 0.086

6.516 ± 0.039

1.005 ± 0.017

4.02 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski