Vibrio phage S4-7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 176 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C9LWF0|A0A1C9LWF0_9CAUD DNA polymerase OS=Vibrio phage S4-7 OX=1873905 PE=4 SV=1
MM1 pKa = 7.54TYY3 pKa = 8.45KK4 pKa = 10.08TLYY7 pKa = 9.92IKK9 pKa = 10.76QNGINYY15 pKa = 8.24TVEE18 pKa = 4.22ADD20 pKa = 2.93ITYY23 pKa = 10.86NPVVCDD29 pKa = 4.39DD30 pKa = 3.71KK31 pKa = 11.44HH32 pKa = 8.07LAMSDD37 pKa = 2.64WDD39 pKa = 4.08TQEE42 pKa = 3.45EE43 pKa = 5.18FYY45 pKa = 10.85IEE47 pKa = 5.55DD48 pKa = 4.06ILVLDD53 pKa = 4.35PNGVDD58 pKa = 3.25VTEE61 pKa = 4.65LVFISDD67 pKa = 3.59EE68 pKa = 4.38SIKK71 pKa = 10.77RR72 pKa = 11.84EE73 pKa = 4.23LDD75 pKa = 2.95MDD77 pKa = 5.39LNDD80 pKa = 4.73CYY82 pKa = 11.6VDD84 pKa = 3.57TKK86 pKa = 10.56EE87 pKa = 5.16LSFFYY92 pKa = 10.94VPEE95 pKa = 4.27EE96 pKa = 3.98KK97 pKa = 10.75LL98 pKa = 3.41

Molecular weight: 11.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C9LW56|A0A1C9LW56_9CAUD Uncharacterized protein OS=Vibrio phage S4-7 OX=1873905 PE=4 SV=1
MM1 pKa = 7.56SGNSYY6 pKa = 9.87TIGQSAAKK14 pKa = 9.21PRR16 pKa = 11.84NRR18 pKa = 11.84LLFSKK23 pKa = 9.66EE24 pKa = 3.92FIMTGEE30 pKa = 3.58WRR32 pKa = 11.84ICLEE36 pKa = 3.97EE37 pKa = 4.33PNYY40 pKa = 10.84SVSNLGRR47 pKa = 11.84IQNNEE52 pKa = 3.41SRR54 pKa = 11.84HH55 pKa = 4.78ISKK58 pKa = 10.65CVMRR62 pKa = 11.84KK63 pKa = 6.45VQRR66 pKa = 11.84LAHH69 pKa = 6.26RR70 pKa = 11.84GVTLKK75 pKa = 10.97

Molecular weight: 8.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

176

0

176

36397

66

847

206.8

23.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.684 ± 0.249

1.797 ± 0.118

6.561 ± 0.146

7.687 ± 0.234

4.399 ± 0.122

6.468 ± 0.201

2.039 ± 0.099

6.303 ± 0.137

7.789 ± 0.253

8.322 ± 0.196

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.44 ± 0.106

5.388 ± 0.135

2.921 ± 0.133

3.206 ± 0.115

4.124 ± 0.151

7.017 ± 0.144

6.146 ± 0.174

6.605 ± 0.18

1.5 ± 0.08

4.605 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski