Sphingobium chlorophenolicum L-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; Sphingobium chlorophenolicum

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4062 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6F0N9|F6F0N9_SPHCR Transcriptional regulator GntR family OS=Sphingobium chlorophenolicum L-1 OX=690566 GN=Sphch_2718 PE=4 SV=1
MM1 pKa = 7.56TYY3 pKa = 10.51GRR5 pKa = 11.84SVTIDD10 pKa = 3.16GSLDD14 pKa = 3.05DD15 pKa = 4.44WLATDD20 pKa = 5.89RR21 pKa = 11.84IDD23 pKa = 5.19DD24 pKa = 3.87NSAPGYY30 pKa = 9.28QVYY33 pKa = 7.91ATLDD37 pKa = 3.03NDD39 pKa = 3.86AFVFALTAPVAIGADD54 pKa = 3.38TTIWLNTDD62 pKa = 3.25GKK64 pKa = 9.43TSTGYY69 pKa = 10.64QIFGFAGGAEE79 pKa = 4.26YY80 pKa = 10.84NVNFAADD87 pKa = 3.92GSVNLYY93 pKa = 10.63SGGAGEE99 pKa = 4.18TLVLGGLQAAWSADD113 pKa = 3.2RR114 pKa = 11.84TTVEE118 pKa = 4.15FRR120 pKa = 11.84LPTASVGNPAAMYY133 pKa = 10.12SYY135 pKa = 11.65YY136 pKa = 10.69DD137 pKa = 3.56INNLSNSFMPSNYY150 pKa = 8.91QSAPFTVFNQTTATQAPDD168 pKa = 3.24MRR170 pKa = 11.84IGIVFSQTTADD181 pKa = 3.82AYY183 pKa = 10.59FSPTAYY189 pKa = 9.99SQLFMSVQAQAIQAGIPFDD208 pKa = 3.74ILTEE212 pKa = 4.37ADD214 pKa = 3.6LTDD217 pKa = 4.26LNKK220 pKa = 10.43LSHH223 pKa = 6.51YY224 pKa = 9.61DD225 pKa = 3.36ALVFPSFRR233 pKa = 11.84NVPVDD238 pKa = 3.18KK239 pKa = 11.31VEE241 pKa = 5.45AITHH245 pKa = 4.9TLEE248 pKa = 4.11MASKK252 pKa = 9.94QLGIGMITAGEE263 pKa = 4.14FMTNQPYY270 pKa = 9.57VQGGAIGGDD279 pKa = 4.03PLPGDD284 pKa = 4.91PYY286 pKa = 11.72AQMKK290 pKa = 10.45LLFDD294 pKa = 3.87ATRR297 pKa = 11.84VTGGTGNVVVTATDD311 pKa = 3.45ANGLVLDD318 pKa = 5.02HH319 pKa = 6.48YY320 pKa = 11.95ANGEE324 pKa = 4.12QVNSYY329 pKa = 9.55TGVGWNAFASVSGTGHH345 pKa = 6.44QIATEE350 pKa = 4.44TINGGSTYY358 pKa = 10.89AAALATQTGAARR370 pKa = 11.84NVLFSSEE377 pKa = 3.75GVMADD382 pKa = 4.33ANMLQQAISYY392 pKa = 8.11AANGTGLSVGLHH404 pKa = 6.6LSRR407 pKa = 11.84EE408 pKa = 4.28SGIVAARR415 pKa = 11.84VDD417 pKa = 3.54MDD419 pKa = 3.35QSQEE423 pKa = 4.11LEE425 pKa = 4.3DD426 pKa = 3.95VNPPGSTPGVYY437 pKa = 10.23DD438 pKa = 3.53KK439 pKa = 11.08LLPILTQWKK448 pKa = 9.71ADD450 pKa = 3.66YY451 pKa = 10.88NFVGSFYY458 pKa = 11.03LNVGNGTNGTGTDD471 pKa = 3.38WAVSLPYY478 pKa = 10.58YY479 pKa = 10.32KK480 pKa = 10.85ALLDD484 pKa = 4.35LGNEE488 pKa = 4.38LGTHH492 pKa = 6.01SLTHH496 pKa = 6.65PEE498 pKa = 3.9NTNLLSASQIQTEE511 pKa = 4.56FQDD514 pKa = 3.1STTILNQQISAYY526 pKa = 10.08LNQPFQITGGAVPGAPEE543 pKa = 4.2SLPVSQEE550 pKa = 3.05IMKK553 pKa = 10.38YY554 pKa = 10.05VSDD557 pKa = 4.24YY558 pKa = 10.76LTGGYY563 pKa = 9.32AAQGAGYY570 pKa = 8.59PNAFGYY576 pKa = 7.46MTPADD581 pKa = 3.36QTKK584 pKa = 10.16IYY586 pKa = 9.14FAPNTSFDD594 pKa = 3.28FTLIEE599 pKa = 4.28FQRR602 pKa = 11.84KK603 pKa = 5.51TVAEE607 pKa = 4.23ATAAWIGEE615 pKa = 3.86FDD617 pKa = 4.33KK618 pKa = 11.45LVANAGTPVIVWPWHH633 pKa = 6.94DD634 pKa = 3.57YY635 pKa = 10.36GAADD639 pKa = 4.71FLSGDD644 pKa = 3.88PTTDD648 pKa = 2.83SPYY651 pKa = 10.66DD652 pKa = 3.37ISLFTSWIQHH662 pKa = 4.4AVAANMEE669 pKa = 4.58FVTLDD674 pKa = 3.39DD675 pKa = 3.79LAARR679 pKa = 11.84MTAVKK684 pKa = 10.21NATITTTVAGNSIAANVSGTNVGNLSLDD712 pKa = 3.43VDD714 pKa = 4.08RR715 pKa = 11.84QGTMVIQNVTGWYY728 pKa = 9.97AYY730 pKa = 10.57DD731 pKa = 3.64SDD733 pKa = 5.55SVFLPQSGGSFQINLGAAADD753 pKa = 4.23DD754 pKa = 3.97VTHH757 pKa = 6.31ITDD760 pKa = 3.66LPMRR764 pKa = 11.84AVLMSVTGDD773 pKa = 3.36GHH775 pKa = 6.49NLAFSVVGEE784 pKa = 4.28GKK786 pKa = 10.38VVIDD790 pKa = 3.9IANPGTDD797 pKa = 4.09GIIVTGASGFTQVGEE812 pKa = 4.2IVTLEE817 pKa = 3.77LGAYY821 pKa = 8.43GLHH824 pKa = 7.09NITLQYY830 pKa = 10.46KK831 pKa = 8.69DD832 pKa = 3.68PPVIVSNGGGATASLSIAEE851 pKa = 4.67NGTAVTTVAATDD863 pKa = 3.62ADD865 pKa = 3.53IGTVITYY872 pKa = 9.65SISGGADD879 pKa = 2.87AAKK882 pKa = 9.11FTIDD886 pKa = 3.23SGTGVLAFATAPNFEE901 pKa = 4.83APTDD905 pKa = 3.66SGPNNVYY912 pKa = 10.67DD913 pKa = 4.28VIVRR917 pKa = 11.84ASDD920 pKa = 3.21GTLFDD925 pKa = 3.76EE926 pKa = 4.46QAIAVTVTDD935 pKa = 3.67VNEE938 pKa = 4.33APVITSNGGGASAAISVPEE957 pKa = 4.25NGTAVTSVTAVDD969 pKa = 3.94PDD971 pKa = 3.42AGTTLVYY978 pKa = 10.63SIVGGPDD985 pKa = 2.84RR986 pKa = 11.84DD987 pKa = 3.46LFVINPSTGALSFLSPPNYY1006 pKa = 10.0DD1007 pKa = 3.99LPGDD1011 pKa = 3.83ANGNNVYY1018 pKa = 10.61NLTVRR1023 pKa = 11.84ASDD1026 pKa = 3.45GALFDD1031 pKa = 4.72DD1032 pKa = 3.93QALNVAVTNVNEE1044 pKa = 4.16SAPVITSGGGGDD1056 pKa = 3.38AAAISVSSGTKK1067 pKa = 9.5PVMKK1071 pKa = 9.59VTAVDD1076 pKa = 3.51PDD1078 pKa = 3.86ANTTLVYY1085 pKa = 10.42SISGGVDD1092 pKa = 2.76AAKK1095 pKa = 9.2FTINASTGQLNFLNTPNFLLPTDD1118 pKa = 3.66MGQNNVYY1125 pKa = 10.51DD1126 pKa = 4.36VIVRR1130 pKa = 11.84ASDD1133 pKa = 3.13GTLYY1137 pKa = 10.68DD1138 pKa = 3.63EE1139 pKa = 4.27QAIAVTVTILNAPPIIISNGGGASATIRR1167 pKa = 11.84MSEE1170 pKa = 3.99NVAAVTKK1177 pKa = 9.29VTATDD1182 pKa = 3.36VNLGQTVTYY1191 pKa = 10.27SLGSAVDD1198 pKa = 3.74GALFAIDD1205 pKa = 3.8AQTGVLTFKK1214 pKa = 10.51AAPDD1218 pKa = 3.88FEE1220 pKa = 4.96TPTDD1224 pKa = 3.75SNLDD1228 pKa = 2.95NRR1230 pKa = 11.84YY1231 pKa = 8.85QVQVLATDD1239 pKa = 3.44SLGAQDD1245 pKa = 4.59FQNLTIVVNNVKK1257 pKa = 10.26GVSLTAASGGSTLTGGMEE1275 pKa = 4.16EE1276 pKa = 4.56DD1277 pKa = 3.7TLKK1280 pKa = 11.14GAGGNDD1286 pKa = 3.19VLIGNGGADD1295 pKa = 2.88ILTGNGGNDD1304 pKa = 4.13SIDD1307 pKa = 3.49GGAGADD1313 pKa = 4.32RR1314 pKa = 11.84ITGGAGADD1322 pKa = 3.79MLTGGADD1329 pKa = 3.53ADD1331 pKa = 4.13LFIYY1335 pKa = 10.23AATGNSTPLVMDD1347 pKa = 5.58RR1348 pKa = 11.84IMDD1351 pKa = 3.88FQDD1354 pKa = 3.41GLDD1357 pKa = 4.49RR1358 pKa = 11.84IDD1360 pKa = 5.12LSAIDD1365 pKa = 4.87ALPALSGNQAFSFIGNAAFSGAGQLHH1391 pKa = 6.89FFTNGTDD1398 pKa = 3.67SFIEE1402 pKa = 4.09ANVDD1406 pKa = 3.47NNPATAEE1413 pKa = 3.83FAIRR1417 pKa = 11.84LVGVHH1422 pKa = 6.39NLTAADD1428 pKa = 3.94FLLL1431 pKa = 4.84

Molecular weight: 148.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6EU80|F6EU80_SPHCR RNA polymerase sigma-24 subunit ECF subfamily OS=Sphingobium chlorophenolicum L-1 OX=690566 GN=Sphch_1018 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.06SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4062

0

4062

1340733

31

4534

330.1

35.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.953 ± 0.057

0.806 ± 0.012

5.943 ± 0.031

5.385 ± 0.034

3.576 ± 0.024

9.031 ± 0.062

2.034 ± 0.022

5.178 ± 0.026

3.035 ± 0.03

9.876 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.577 ± 0.023

2.668 ± 0.029

5.277 ± 0.031

3.228 ± 0.019

7.298 ± 0.037

5.398 ± 0.032

5.038 ± 0.035

6.99 ± 0.029

1.406 ± 0.015

2.303 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski