Porphyromonas sp. COT-239 OH1446

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Porphyromonadaceae; Porphyromonas; unclassified Porphyromonas

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1412 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A2E0K7|A0A0A2E0K7_9PORP Uncharacterized protein OS=Porphyromonas sp. COT-239 OH1446 OX=1515613 GN=HQ37_03290 PE=4 SV=1
MM1 pKa = 7.85PEE3 pKa = 3.37VALQFVEE10 pKa = 5.05EE11 pKa = 4.09VLPYY15 pKa = 10.8GDD17 pKa = 3.35VFAFEE22 pKa = 5.24APMGTGKK29 pKa = 7.7TTFIKK34 pKa = 10.52ALCLALGVQDD44 pKa = 4.85VINSPTFSIINEE56 pKa = 3.82YY57 pKa = 9.92RR58 pKa = 11.84AEE60 pKa = 4.1PSGEE64 pKa = 4.16LIYY67 pKa = 11.12HH68 pKa = 6.23FDD70 pKa = 3.62CYY72 pKa = 10.64RR73 pKa = 11.84LEE75 pKa = 4.32RR76 pKa = 11.84LDD78 pKa = 4.51DD79 pKa = 4.31ALSLGVEE86 pKa = 4.89DD87 pKa = 4.38YY88 pKa = 11.37LEE90 pKa = 4.54SGALCLIEE98 pKa = 4.15WPKK101 pKa = 10.8LLEE104 pKa = 5.11PILPSDD110 pKa = 3.71TVLVEE115 pKa = 4.62LSLEE119 pKa = 4.05ADD121 pKa = 3.53GQGRR125 pKa = 11.84ILTAHH130 pKa = 5.8YY131 pKa = 11.15NEE133 pKa = 4.47VV134 pKa = 3.15

Molecular weight: 14.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A2DX28|A0A0A2DX28_9PORP Beta-N-acetylhexosaminidase OS=Porphyromonas sp. COT-239 OH1446 OX=1515613 GN=HQ37_05180 PE=3 SV=1
MM1 pKa = 7.57KK2 pKa = 9.99IARR5 pKa = 11.84VTPLMRR11 pKa = 11.84ALLLFIACLCAVNSLSAQGFSLSSGSSPQPRR42 pKa = 11.84PTDD45 pKa = 3.15SLRR48 pKa = 11.84ISRR51 pKa = 11.84RR52 pKa = 11.84LRR54 pKa = 11.84PEE56 pKa = 3.64VKK58 pKa = 10.15SRR60 pKa = 11.84LLPQQSYY67 pKa = 10.73GINAHH72 pKa = 6.22RR73 pKa = 11.84RR74 pKa = 11.84TMLIRR79 pKa = 11.84PEE81 pKa = 4.2FGVLPIRR88 pKa = 11.84DD89 pKa = 3.63GSTIILNGEE98 pKa = 4.06TPIASYY104 pKa = 10.93EE105 pKa = 4.03PLDD108 pKa = 4.14PRR110 pKa = 11.84PSSIRR115 pKa = 11.84DD116 pKa = 3.24LSPRR120 pKa = 11.84GIRR123 pKa = 11.84QSSGIRR129 pKa = 11.84LMGRR133 pKa = 11.84TLYY136 pKa = 10.76FSDD139 pKa = 3.32PAEE142 pKa = 3.97RR143 pKa = 11.84VEE145 pKa = 5.5LIDD148 pKa = 3.42VTGKK152 pKa = 10.35VVLAYY157 pKa = 10.69RR158 pKa = 11.84NVRR161 pKa = 11.84EE162 pKa = 4.27AKK164 pKa = 10.42LNLPEE169 pKa = 5.11GIYY172 pKa = 10.39IMRR175 pKa = 11.84CNINDD180 pKa = 4.16DD181 pKa = 4.02YY182 pKa = 11.31TVSRR186 pKa = 11.84LILRR190 pKa = 4.21

Molecular weight: 21.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1412

0

1412

513336

38

2236

363.6

40.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.121 ± 0.069

1.01 ± 0.024

5.003 ± 0.041

7.186 ± 0.066

3.774 ± 0.035

7.315 ± 0.047

2.311 ± 0.025

6.32 ± 0.048

4.533 ± 0.064

10.975 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.544 ± 0.031

3.242 ± 0.043

4.233 ± 0.038

3.837 ± 0.036

6.716 ± 0.053

6.851 ± 0.063

4.744 ± 0.033

6.207 ± 0.046

1.131 ± 0.025

3.947 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski