Enterococcus phage phiEF24C (Enterococcus bacteriophage phi-EF24C)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Brockvirinae; Kochikohdavirus; Enterococcus virus EF24C

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 221 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8E2J8|A8E2J8_BPPHE Uncharacterized protein OS=Enterococcus phage phiEF24C OX=442493 GN=EFP_146 PE=4 SV=1
MM1 pKa = 7.61TEE3 pKa = 4.24FANMNKK9 pKa = 9.96EE10 pKa = 3.98EE11 pKa = 4.38LLEE14 pKa = 4.9LLNDD18 pKa = 3.61WFGVSDD24 pKa = 4.2YY25 pKa = 10.37DD26 pKa = 4.13TVMEE30 pKa = 4.72EE31 pKa = 4.06LGEE34 pKa = 4.11MKK36 pKa = 10.25QVTFTGSTNQPLLGGNNDD54 pKa = 5.19LISLPQFFKK63 pKa = 11.31NNEE66 pKa = 4.01ADD68 pKa = 4.2SEE70 pKa = 4.54FPTYY74 pKa = 10.76EE75 pKa = 3.88EE76 pKa = 4.48LLEE79 pKa = 4.22EE80 pKa = 4.89LEE82 pKa = 4.13KK83 pKa = 10.88DD84 pKa = 3.29TWNLEE89 pKa = 4.27AEE91 pKa = 4.5DD92 pKa = 3.41NTYY95 pKa = 11.24NYY97 pKa = 11.07GGFLEE102 pKa = 5.21RR103 pKa = 11.84DD104 pKa = 3.1MDD106 pKa = 4.09FKK108 pKa = 11.34VIQAEE113 pKa = 4.17NSDD116 pKa = 3.42TTIAFFAIHH125 pKa = 6.3TGVDD129 pKa = 3.2VRR131 pKa = 11.84AGYY134 pKa = 10.47SKK136 pKa = 10.73AIPVIFEE143 pKa = 4.04TYY145 pKa = 10.19YY146 pKa = 11.28DD147 pKa = 3.7FDD149 pKa = 4.32EE150 pKa = 4.72FLGNYY155 pKa = 9.04FYY157 pKa = 11.39SQGYY161 pKa = 8.4YY162 pKa = 10.5AFKK165 pKa = 10.46HH166 pKa = 6.05DD167 pKa = 3.87NKK169 pKa = 10.44EE170 pKa = 3.9YY171 pKa = 10.31TISLDD176 pKa = 3.28VSAVSEE182 pKa = 4.06YY183 pKa = 10.52VRR185 pKa = 11.84IYY187 pKa = 10.44IADD190 pKa = 3.76EE191 pKa = 4.11NNEE194 pKa = 3.98EE195 pKa = 4.01LQQVYY200 pKa = 8.03EE201 pKa = 4.45QEE203 pKa = 4.07TCMDD207 pKa = 4.24LDD209 pKa = 3.88KK210 pKa = 11.42EE211 pKa = 4.61SVAEE215 pKa = 3.8YY216 pKa = 11.07LEE218 pKa = 4.1WQKK221 pKa = 10.84IEE223 pKa = 4.55FKK225 pKa = 10.61DD226 pKa = 4.32LKK228 pKa = 10.5PALL231 pKa = 4.04

Molecular weight: 26.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8E277|A8E277_BPPHE Uncharacterized protein OS=Enterococcus phage phiEF24C OX=442493 GN=EFP_025 PE=4 SV=1
MM1 pKa = 7.32ARR3 pKa = 11.84KK4 pKa = 8.59GQRR7 pKa = 11.84PVLFTDD13 pKa = 3.92SKK15 pKa = 11.52AILGNLTRR23 pKa = 11.84AVVDD27 pKa = 4.04EE28 pKa = 4.49VLSDD32 pKa = 3.76AQDD35 pKa = 3.1VALRR39 pKa = 11.84NGSSVQRR46 pKa = 11.84MPSYY50 pKa = 11.07LIVTEE55 pKa = 4.18SRR57 pKa = 11.84MAKK60 pKa = 10.3NGVIDD65 pKa = 4.76LKK67 pKa = 11.18PFFARR72 pKa = 11.84SNKK75 pKa = 9.5KK76 pKa = 9.97KK77 pKa = 10.28YY78 pKa = 9.02NKK80 pKa = 9.77KK81 pKa = 10.5GEE83 pKa = 4.0WYY85 pKa = 9.87LYY87 pKa = 10.29IPISMKK93 pKa = 8.85TRR95 pKa = 11.84NMSRR99 pKa = 11.84RR100 pKa = 11.84LYY102 pKa = 10.69DD103 pKa = 3.32EE104 pKa = 4.78LRR106 pKa = 11.84AVPVGTKK113 pKa = 9.89PVTVKK118 pKa = 9.86MDD120 pKa = 3.45YY121 pKa = 10.83LYY123 pKa = 10.88DD124 pKa = 3.64RR125 pKa = 11.84RR126 pKa = 11.84KK127 pKa = 9.8QSPSVSSINYY137 pKa = 8.74KK138 pKa = 10.25PKK140 pKa = 8.93STNVTVIPQSWGKK153 pKa = 7.84GTRR156 pKa = 11.84NTYY159 pKa = 9.47VAFRR163 pKa = 11.84TVNANSPANSWIINRR178 pKa = 11.84RR179 pKa = 11.84NVNDD183 pKa = 4.21DD184 pKa = 4.03DD185 pKa = 3.93MSKK188 pKa = 9.59TMLRR192 pKa = 11.84NIDD195 pKa = 4.92RR196 pKa = 11.84LMKK199 pKa = 10.14WKK201 pKa = 10.49LKK203 pKa = 10.69NLGGG207 pKa = 3.72

Molecular weight: 23.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

221

0

221

42234

23

1825

191.1

21.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.484 ± 0.185

0.777 ± 0.079

6.168 ± 0.159

8.844 ± 0.293

3.751 ± 0.105

6.208 ± 0.213

1.473 ± 0.083

6.634 ± 0.151

7.923 ± 0.214

8.661 ± 0.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.555 ± 0.069

5.813 ± 0.17

2.983 ± 0.141

3.431 ± 0.188

3.999 ± 0.119

6.199 ± 0.163

6.289 ± 0.23

7.056 ± 0.185

1.082 ± 0.073

4.672 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski