Clostridium virus phiC2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Lubbockvirus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3QSB6|A3QSB6_9CAUD Uncharacterized protein OS=Clostridium virus phiC2 OX=262071 GN=phiC2p26 PE=4 SV=1
MM1 pKa = 7.17TFEE4 pKa = 5.01NIIKK8 pKa = 10.43RR9 pKa = 11.84MLDD12 pKa = 3.5SVPDD16 pKa = 3.75TFDD19 pKa = 3.13KK20 pKa = 11.3RR21 pKa = 11.84EE22 pKa = 3.55GSIIYY27 pKa = 9.95NALAPVAIEE36 pKa = 3.84LTEE39 pKa = 4.1TYY41 pKa = 10.56IAMDD45 pKa = 3.97EE46 pKa = 4.57LLDD49 pKa = 3.61QTFVDD54 pKa = 3.99TASYY58 pKa = 10.44YY59 pKa = 10.63YY60 pKa = 10.6LEE62 pKa = 4.53KK63 pKa = 10.36RR64 pKa = 11.84CKK66 pKa = 9.96EE67 pKa = 3.81RR68 pKa = 11.84GITPLEE74 pKa = 4.12ATNTIAKK81 pKa = 9.82GVFNIDD87 pKa = 2.77IPIDD91 pKa = 3.41SRR93 pKa = 11.84FNLGEE98 pKa = 3.92YY99 pKa = 10.21NYY101 pKa = 10.57VAIEE105 pKa = 4.44RR106 pKa = 11.84ISEE109 pKa = 4.45GIYY112 pKa = 10.66KK113 pKa = 8.15MKK115 pKa = 10.65CEE117 pKa = 3.7TAGPIFEE124 pKa = 5.41LGQLIPIEE132 pKa = 4.6YY133 pKa = 9.16IDD135 pKa = 3.91KK136 pKa = 11.27LEE138 pKa = 4.1TAEE141 pKa = 4.15LTEE144 pKa = 3.9ILINGEE150 pKa = 4.21DD151 pKa = 4.01EE152 pKa = 4.17EE153 pKa = 5.77SEE155 pKa = 4.06DD156 pKa = 4.37SLRR159 pKa = 11.84QRR161 pKa = 11.84YY162 pKa = 8.8YY163 pKa = 11.4DD164 pKa = 3.62SLNSQSFGGNIQNYY178 pKa = 8.46KK179 pKa = 11.05DD180 pKa = 4.18EE181 pKa = 4.39VNKK184 pKa = 10.44IQDD187 pKa = 3.33VGGVKK192 pKa = 10.13VYY194 pKa = 9.49PVWDD198 pKa = 3.56GGGTVKK204 pKa = 10.7LVIINSNFKK213 pKa = 10.69VPSEE217 pKa = 4.02DD218 pKa = 3.66LVNLVQEE225 pKa = 4.72EE226 pKa = 4.24IDD228 pKa = 4.3PIGHH232 pKa = 6.08QGQGLGLAPIGHH244 pKa = 7.0KK245 pKa = 9.54VTVTGVVSTTINISADD261 pKa = 2.81ITYY264 pKa = 10.73KK265 pKa = 10.73NGYY268 pKa = 4.57TWEE271 pKa = 4.41NIKK274 pKa = 10.79SIAEE278 pKa = 4.07EE279 pKa = 4.05AVDD282 pKa = 5.51DD283 pKa = 4.24YY284 pKa = 11.94LNEE287 pKa = 4.92LNMSWEE293 pKa = 4.23DD294 pKa = 3.68EE295 pKa = 4.15EE296 pKa = 4.54NLIVRR301 pKa = 11.84ISQIEE306 pKa = 4.13TRR308 pKa = 11.84LLSIDD313 pKa = 3.6GVLDD317 pKa = 3.37IANTMINEE325 pKa = 4.26VKK327 pKa = 10.84SNLTINSNSIVVRR340 pKa = 11.84GEE342 pKa = 3.98VVGG345 pKa = 4.01

Molecular weight: 38.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3QSE6|A3QSE6_9CAUD Putative repressor OS=Clostridium virus phiC2 OX=262071 GN=phiC2p58 PE=4 SV=1
MM1 pKa = 7.54VIPIAKK7 pKa = 8.09NTKK10 pKa = 7.47QTSKK14 pKa = 9.62TVASKK19 pKa = 10.82ASKK22 pKa = 9.49ILKK25 pKa = 9.59DD26 pKa = 3.58GRR28 pKa = 11.84YY29 pKa = 8.54SAASKK34 pKa = 10.58SVAGSALSQTKK45 pKa = 10.13KK46 pKa = 10.61GPKK49 pKa = 9.17KK50 pKa = 10.55

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

14570

15

762

177.7

20.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.873 ± 0.511

1.256 ± 0.143

6.253 ± 0.219

8.586 ± 0.409

4.077 ± 0.175

5.388 ± 0.268

1.139 ± 0.106

9.06 ± 0.316

10.782 ± 0.45

8.428 ± 0.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.128 ± 0.128

7.268 ± 0.211

1.99 ± 0.134

2.91 ± 0.172

3.713 ± 0.192

5.765 ± 0.209

5.333 ± 0.281

5.765 ± 0.234

0.927 ± 0.079

4.358 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski