Gemmatirosa kalamazoonesis

Taxonomy: cellular organisms; Bacteria; FCB group; Gemmatimonadetes; Gemmatimonadetes; Gemmatimonadales; Gemmatimonadaceae; Gemmatirosa

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6261 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0RNZ4|W0RNZ4_9BACT Uncharacterized protein OS=Gemmatirosa kalamazoonesis OX=861299 GN=J421_5179 PE=4 SV=1
MM1 pKa = 7.16STTTQSDD8 pKa = 3.78LVLTVTSVATAEE20 pKa = 4.33VKK22 pKa = 10.65KK23 pKa = 11.09FMDD26 pKa = 4.2AEE28 pKa = 4.56GVSYY32 pKa = 10.6DD33 pKa = 3.47QGGLRR38 pKa = 11.84VSVQPGGCSGFKK50 pKa = 10.27YY51 pKa = 10.74GLLIEE56 pKa = 4.91DD57 pKa = 4.23QPAEE61 pKa = 4.38DD62 pKa = 5.08DD63 pKa = 4.87LVLAQDD69 pKa = 4.18GFNVFVDD76 pKa = 4.3PFSAQYY82 pKa = 10.77INGVTIDD89 pKa = 4.45YY90 pKa = 9.69VSSMQGSGFTFKK102 pKa = 10.98NPNATGGCGCGSSFSAA118 pKa = 4.79

Molecular weight: 12.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0RIS9|W0RIS9_9BACT Thymidine kinase OS=Gemmatirosa kalamazoonesis OX=861299 GN=tdk PE=3 SV=1
MM1 pKa = 7.51AASKK5 pKa = 9.65TSSKK9 pKa = 11.02GGAKK13 pKa = 9.95KK14 pKa = 10.19GARR17 pKa = 11.84KK18 pKa = 9.97GGAKK22 pKa = 10.13KK23 pKa = 10.6GGAKK27 pKa = 10.16KK28 pKa = 10.57GGAKK32 pKa = 9.87RR33 pKa = 11.84GSAKK37 pKa = 10.46KK38 pKa = 10.05GGARR42 pKa = 11.84KK43 pKa = 9.99GSAKK47 pKa = 10.43KK48 pKa = 10.59GGAKK52 pKa = 9.94RR53 pKa = 11.84GGAKK57 pKa = 10.18KK58 pKa = 9.07STAKK62 pKa = 10.26RR63 pKa = 11.84ASAKK67 pKa = 10.4KK68 pKa = 9.45GGARR72 pKa = 11.84KK73 pKa = 9.05GASKK77 pKa = 10.86GGAKK81 pKa = 9.86RR82 pKa = 11.84GSAKK86 pKa = 10.46KK87 pKa = 10.33GGARR91 pKa = 11.84KK92 pKa = 9.75GGRR95 pKa = 11.84KK96 pKa = 9.03SAGSSGAAAGGATGGAAA113 pKa = 3.55

Molecular weight: 10.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6261

0

6261

2262513

33

3269

361.4

38.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.626 ± 0.057

0.668 ± 0.009

5.86 ± 0.022

4.85 ± 0.028

3.066 ± 0.016

8.839 ± 0.029

2.008 ± 0.014

3.237 ± 0.02

1.75 ± 0.024

10.059 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.761 ± 0.012

2.003 ± 0.019

5.946 ± 0.026

2.599 ± 0.019

9.211 ± 0.036

4.998 ± 0.024

5.991 ± 0.029

8.808 ± 0.031

1.495 ± 0.014

2.225 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski