Pseudoclostridium thermosuccinogenes

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Pseudoclostridium

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3781 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K2FJ23|A0A2K2FJ23_9FIRM Uncharacterized protein OS=Pseudoclostridium thermosuccinogenes OX=84032 GN=CDQ84_04940 PE=4 SV=1
MM1 pKa = 7.75SYY3 pKa = 10.79LKK5 pKa = 10.78EE6 pKa = 3.66KK7 pKa = 10.37VAYY10 pKa = 10.25LKK12 pKa = 11.08GLAEE16 pKa = 4.32GMQINDD22 pKa = 3.59ASNEE26 pKa = 3.92GKK28 pKa = 10.18LLKK31 pKa = 10.65AIIDD35 pKa = 3.93VLDD38 pKa = 5.24DD39 pKa = 3.24IALEE43 pKa = 3.96VDD45 pKa = 4.95DD46 pKa = 4.19IRR48 pKa = 11.84EE49 pKa = 4.04VQEE52 pKa = 3.9QLNEE56 pKa = 3.99QVDD59 pKa = 4.28NIDD62 pKa = 3.19EE63 pKa = 4.5DD64 pKa = 3.79LAEE67 pKa = 4.09VEE69 pKa = 5.47RR70 pKa = 11.84IIFDD74 pKa = 4.42DD75 pKa = 4.16EE76 pKa = 3.97EE77 pKa = 5.42DD78 pKa = 3.87YY79 pKa = 10.88PALVEE84 pKa = 4.94CPYY87 pKa = 10.07CHH89 pKa = 7.47KK90 pKa = 10.47EE91 pKa = 4.0FEE93 pKa = 4.29VDD95 pKa = 3.43EE96 pKa = 4.99DD97 pKa = 5.07LIDD100 pKa = 5.26DD101 pKa = 4.22EE102 pKa = 6.61GDD104 pKa = 3.65TIEE107 pKa = 5.83CPHH110 pKa = 6.38CHH112 pKa = 6.5KK113 pKa = 10.77KK114 pKa = 10.5VDD116 pKa = 3.67IDD118 pKa = 3.63WDD120 pKa = 4.41CEE122 pKa = 4.19CEE124 pKa = 4.02EE125 pKa = 5.43CGDD128 pKa = 4.98DD129 pKa = 5.48DD130 pKa = 5.33HH131 pKa = 8.96DD132 pKa = 5.17HH133 pKa = 7.22DD134 pKa = 6.16AEE136 pKa = 4.17

Molecular weight: 15.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K2EWT5|A0A2K2EWT5_9FIRM Sugar ABC transporter permease OS=Pseudoclostridium thermosuccinogenes OX=84032 GN=CDQ84_04175 PE=3 SV=1
MM1 pKa = 7.65LYY3 pKa = 10.7SSFFKK8 pKa = 10.85LQVRR12 pKa = 11.84CRR14 pKa = 11.84CFLGRR19 pKa = 11.84MFWLRR24 pKa = 11.84DD25 pKa = 3.3SPLRR29 pKa = 11.84DD30 pKa = 3.34SPFQGQRR37 pKa = 11.84EE38 pKa = 4.08RR39 pKa = 11.84GAKK42 pKa = 8.36WLTVLCCSMKK52 pKa = 10.28SIEE55 pKa = 3.94YY56 pKa = 10.35RR57 pKa = 11.84ITRR60 pKa = 11.84QQNASAGFFRR70 pKa = 11.84FLRR73 pKa = 11.84VCC75 pKa = 3.99

Molecular weight: 9.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3781

0

3781

1216124

19

2930

321.6

36.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.836 ± 0.039

1.208 ± 0.017

5.741 ± 0.033

7.071 ± 0.046

4.325 ± 0.03

6.877 ± 0.035

1.593 ± 0.017

8.396 ± 0.037

7.401 ± 0.037

9.009 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.85 ± 0.017

4.867 ± 0.025

3.473 ± 0.022

2.682 ± 0.017

4.473 ± 0.031

6.284 ± 0.035

4.922 ± 0.029

6.763 ± 0.032

0.993 ± 0.016

4.234 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski