Apis mellifera associated microvirus 35

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5V0|A0A3Q8U5V0_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 35 OX=2494764 PE=3 SV=1
MM1 pKa = 7.64SEE3 pKa = 3.81KK4 pKa = 9.92TKK6 pKa = 10.9QFVGSVKK13 pKa = 10.6AKK15 pKa = 10.02TDD17 pKa = 3.72FAVLNNADD25 pKa = 3.76IDD27 pKa = 3.97RR28 pKa = 11.84DD29 pKa = 3.94QIKK32 pKa = 9.87EE33 pKa = 4.04WLVQDD38 pKa = 3.98VKK40 pKa = 10.98RR41 pKa = 11.84VAILVNDD48 pKa = 3.95ILNTPEE54 pKa = 4.88CIEE57 pKa = 3.99NLTDD61 pKa = 3.41VFYY64 pKa = 11.23KK65 pKa = 10.44RR66 pKa = 11.84YY67 pKa = 9.73QDD69 pKa = 3.38FHH71 pKa = 8.63KK72 pKa = 10.77EE73 pKa = 3.73NAIKK77 pKa = 10.41EE78 pKa = 4.28MAEE81 pKa = 3.79KK82 pKa = 10.36DD83 pKa = 3.81YY84 pKa = 11.22STVEE88 pKa = 3.87QNGG91 pKa = 3.19

Molecular weight: 10.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8U6F5|A0A3Q8U6F5_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 35 OX=2494764 PE=4 SV=1
MM1 pKa = 7.7EE2 pKa = 5.3CLKK5 pKa = 10.1MIKK8 pKa = 8.7VTHH11 pKa = 6.58KK12 pKa = 10.62GVTHH16 pKa = 6.34TVPCGKK22 pKa = 10.08CAFCFSNKK30 pKa = 8.9RR31 pKa = 11.84SSWMFRR37 pKa = 11.84IHH39 pKa = 7.91HH40 pKa = 5.89EE41 pKa = 4.19MRR43 pKa = 11.84TQLHH47 pKa = 6.01KK48 pKa = 10.96GYY50 pKa = 9.96FLTLTYY56 pKa = 10.5DD57 pKa = 3.4EE58 pKa = 4.66KK59 pKa = 10.91HH60 pKa = 5.83VKK62 pKa = 8.59RR63 pKa = 11.84TAEE66 pKa = 3.83GRR68 pKa = 11.84LSLRR72 pKa = 11.84FRR74 pKa = 11.84DD75 pKa = 3.58VQLWLKK81 pKa = 9.12VLRR84 pKa = 11.84KK85 pKa = 9.02RR86 pKa = 11.84KK87 pKa = 10.14YY88 pKa = 7.37YY89 pKa = 9.82VKK91 pKa = 10.28YY92 pKa = 10.32IIVGEE97 pKa = 4.2YY98 pKa = 10.39GSEE101 pKa = 4.08TKK103 pKa = 10.34RR104 pKa = 11.84PHH106 pKa = 4.62YY107 pKa = 9.99HH108 pKa = 6.0ALIWTDD114 pKa = 3.73CAPEE118 pKa = 3.96MLEE121 pKa = 4.02KK122 pKa = 10.51LWFRR126 pKa = 11.84GRR128 pKa = 11.84IHH130 pKa = 7.2FGKK133 pKa = 8.77LTMASAMYY141 pKa = 7.83TLKK144 pKa = 11.14YY145 pKa = 10.1IIQPKK150 pKa = 9.76QKK152 pKa = 10.48DD153 pKa = 3.7EE154 pKa = 4.48NGVEE158 pKa = 4.02RR159 pKa = 11.84TRR161 pKa = 11.84AQFSKK166 pKa = 11.0GLGLAYY172 pKa = 10.56LDD174 pKa = 3.53TAAYY178 pKa = 9.96NYY180 pKa = 8.14HH181 pKa = 5.87TFDD184 pKa = 3.55YY185 pKa = 10.39DD186 pKa = 3.68SPEE189 pKa = 3.95MFSIIDD195 pKa = 3.76GKK197 pKa = 10.79KK198 pKa = 8.68VALPRR203 pKa = 11.84YY204 pKa = 8.04YY205 pKa = 10.0RR206 pKa = 11.84QKK208 pKa = 10.79IFTKK212 pKa = 10.52YY213 pKa = 10.11QLTKK217 pKa = 9.97HH218 pKa = 4.91AHH220 pKa = 5.44KK221 pKa = 10.72AKK223 pKa = 9.32WDD225 pKa = 3.98SIRR228 pKa = 11.84KK229 pKa = 8.22RR230 pKa = 11.84RR231 pKa = 11.84ARR233 pKa = 11.84MRR235 pKa = 11.84EE236 pKa = 3.72LKK238 pKa = 9.36MQGISNTKK246 pKa = 9.61VYY248 pKa = 9.42MHH250 pKa = 7.68ALRR253 pKa = 11.84VDD255 pKa = 3.17HH256 pKa = 7.26AKK258 pKa = 10.49RR259 pKa = 11.84IIEE262 pKa = 4.0NTKK265 pKa = 10.19YY266 pKa = 10.7GQSLL270 pKa = 3.48

Molecular weight: 32.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1366

91

535

273.2

31.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.174 ± 0.505

0.586 ± 0.313

5.71 ± 0.796

6.442 ± 0.654

4.246 ± 0.642

6.735 ± 1.007

2.416 ± 0.655

5.198 ± 0.341

6.515 ± 1.826

7.687 ± 0.638

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.075 ± 0.337

4.319 ± 0.712

4.466 ± 0.941

4.905 ± 0.881

7.028 ± 0.73

5.71 ± 0.49

5.198 ± 0.462

6.296 ± 0.699

1.391 ± 0.276

4.905 ± 0.582
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski