Geodermatophilus ruber

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Geodermatophilus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4702 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4IIM6|A0A1I4IIM6_9ACTN Cob(I)alamin adenosyltransferase OS=Geodermatophilus ruber OX=504800 GN=SAMN04488085_11311 PE=4 SV=1
MM1 pKa = 7.35RR2 pKa = 11.84QVQRR6 pKa = 11.84RR7 pKa = 11.84ALAAAAAGSMLLAGCATTVTGTAAAAHH34 pKa = 7.06PLTDD38 pKa = 3.62VPAEE42 pKa = 4.01ALPITGAGDD51 pKa = 3.46GAIDD55 pKa = 3.6VLARR59 pKa = 11.84NAMADD64 pKa = 3.47LLTYY68 pKa = 9.05WEE70 pKa = 3.99RR71 pKa = 11.84TYY73 pKa = 11.11PEE75 pKa = 5.08LYY77 pKa = 10.65GEE79 pKa = 4.31PFVPLEE85 pKa = 4.1GGIFAVDD92 pKa = 4.58ADD94 pKa = 4.33DD95 pKa = 5.91LDD97 pKa = 4.22PAVYY101 pKa = 9.4PDD103 pKa = 3.83TGIGCDD109 pKa = 4.08RR110 pKa = 11.84LPVDD114 pKa = 3.61PSEE117 pKa = 4.46VEE119 pKa = 4.17GNAFYY124 pKa = 11.15LLPCDD129 pKa = 3.96LVAYY133 pKa = 10.05DD134 pKa = 3.62SALIEE139 pKa = 4.21EE140 pKa = 4.84LAAAHH145 pKa = 6.44GRR147 pKa = 11.84FLGPAVMAHH156 pKa = 5.74EE157 pKa = 5.05MGHH160 pKa = 7.21AIQGRR165 pKa = 11.84NGTWDD170 pKa = 3.43TVDD173 pKa = 3.88TIVSEE178 pKa = 4.33TQADD182 pKa = 4.12CFAGAWTRR190 pKa = 11.84WVADD194 pKa = 3.49GNAAHH199 pKa = 6.75SSLRR203 pKa = 11.84VPEE206 pKa = 4.26LDD208 pKa = 3.29EE209 pKa = 4.21VVVGFLEE216 pKa = 4.49LRR218 pKa = 11.84DD219 pKa = 3.84PVGTGTDD226 pKa = 3.02EE227 pKa = 5.08DD228 pKa = 4.62GAHH231 pKa = 6.62GSGFDD236 pKa = 3.23RR237 pKa = 11.84VSGFFAGWDD246 pKa = 3.63GGATACRR253 pKa = 11.84DD254 pKa = 3.5EE255 pKa = 4.53FDD257 pKa = 3.56EE258 pKa = 5.74DD259 pKa = 4.2RR260 pKa = 11.84LFTAAEE266 pKa = 4.17FTDD269 pKa = 4.53HH270 pKa = 7.29GDD272 pKa = 4.33LEE274 pKa = 4.73NEE276 pKa = 4.14GNASYY281 pKa = 11.35DD282 pKa = 3.57EE283 pKa = 4.21TLLIVDD289 pKa = 4.2ASLPLFYY296 pKa = 10.86EE297 pKa = 4.53SIFPSEE303 pKa = 3.97FGTRR307 pKa = 11.84FQVPALEE314 pKa = 4.9AFDD317 pKa = 4.48GTAPDD322 pKa = 4.0CGEE325 pKa = 4.39LGAHH329 pKa = 6.23GRR331 pKa = 11.84DD332 pKa = 3.5LGYY335 pKa = 10.6CGADD339 pKa = 3.0GTVYY343 pKa = 10.55FDD345 pKa = 3.51EE346 pKa = 4.98TDD348 pKa = 4.21LIEE351 pKa = 4.17PAYY354 pKa = 10.7AEE356 pKa = 4.58LGDD359 pKa = 3.78FAVATGIALPYY370 pKa = 10.52ALAARR375 pKa = 11.84DD376 pKa = 3.78QLGLSTDD383 pKa = 3.72DD384 pKa = 3.75GAATRR389 pKa = 11.84SAVCLTGWYY398 pKa = 7.87TADD401 pKa = 3.82FFAGDD406 pKa = 3.71FRR408 pKa = 11.84EE409 pKa = 4.5VTSLSPGDD417 pKa = 3.31VDD419 pKa = 3.7EE420 pKa = 5.33AVSFLLTYY428 pKa = 10.2GQTEE432 pKa = 4.29SVLPDD437 pKa = 3.1TGLSGFEE444 pKa = 3.68LVGAFRR450 pKa = 11.84DD451 pKa = 4.22GFLHH455 pKa = 7.16GGGGCDD461 pKa = 3.45VGLL464 pKa = 4.23

Molecular weight: 48.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3YRY7|A0A1I3YRY7_9ACTN Uncharacterized protein OS=Geodermatophilus ruber OX=504800 GN=SAMN04488085_101156 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.25TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4702

0

4702

1494515

29

2589

317.8

33.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.374 ± 0.056

0.709 ± 0.009

6.033 ± 0.027

5.747 ± 0.035

2.613 ± 0.023

9.623 ± 0.034

2.044 ± 0.016

2.981 ± 0.028

1.377 ± 0.023

10.704 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.644 ± 0.014

1.491 ± 0.018

6.378 ± 0.039

2.745 ± 0.021

8.289 ± 0.035

4.631 ± 0.021

5.83 ± 0.024

9.532 ± 0.039

1.461 ± 0.015

1.794 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski