Pig stool associated circular ssDNA virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Drosmacovirus; unclassified Drosmacovirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7RMG2|K7RMG2_9VIRU Uncharacterized protein OS=Pig stool associated circular ssDNA virus OX=1249501 PE=4 SV=1
MM1 pKa = 7.46GWFSDD6 pKa = 5.17GIDD9 pKa = 3.46WYY11 pKa = 11.46NKK13 pKa = 9.21YY14 pKa = 10.66INGASNAGWKK24 pKa = 10.4AVMDD28 pKa = 4.55LVGLDD33 pKa = 3.23QADD36 pKa = 3.7ANRR39 pKa = 11.84AQSLLSDD46 pKa = 3.77VPVIGDD52 pKa = 3.47VLQLQQRR59 pKa = 11.84QEE61 pKa = 3.97QISDD65 pKa = 3.54YY66 pKa = 11.14LHH68 pKa = 6.63NKK70 pKa = 9.25DD71 pKa = 4.6MDD73 pKa = 3.67WSDD76 pKa = 3.33VKK78 pKa = 11.28YY79 pKa = 9.69PALIGGGAGRR89 pKa = 11.84SLTSAKK95 pKa = 10.6NFVSKK100 pKa = 10.81NIEE103 pKa = 3.87NLYY106 pKa = 10.53RR107 pKa = 11.84DD108 pKa = 3.5

Molecular weight: 12.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7QZD5|K7QZD5_9VIRU Uncharacterized protein OS=Pig stool associated circular ssDNA virus OX=1249501 PE=4 SV=1
MM1 pKa = 7.31TFQHH5 pKa = 6.87RR6 pKa = 11.84SEE8 pKa = 4.03RR9 pKa = 11.84WGLLQYY15 pKa = 10.5TPLLCTVLTVFGADD29 pKa = 3.67LSRR32 pKa = 11.84TSSSLGSSTVMSQWLVHH49 pKa = 6.35PCSQLTLSKK58 pKa = 10.87LEE60 pKa = 3.71QRR62 pKa = 11.84LAILHH67 pKa = 6.22LRR69 pKa = 11.84ICSILSCTRR78 pKa = 11.84RR79 pKa = 11.84FPMTRR84 pKa = 11.84STTSSTVSWFTRR96 pKa = 11.84TILLLLTVLPLFLLTVPILGFLRR119 pKa = 11.84HH120 pKa = 5.75RR121 pKa = 11.84RR122 pKa = 11.84ISTSTTLYY130 pKa = 10.12SQIRR134 pKa = 11.84MDD136 pKa = 3.81GRR138 pKa = 11.84SPCLSPDD145 pKa = 3.67LPCRR149 pKa = 11.84VFTLWFSPFF158 pKa = 3.21

Molecular weight: 18.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

940

104

333

188.0

21.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.213 ± 0.939

1.489 ± 0.53

5.957 ± 1.199

3.723 ± 1.261

5.0 ± 0.532

5.745 ± 0.771

1.702 ± 0.48

5.213 ± 0.364

4.255 ± 0.716

9.681 ± 1.749

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.085 ± 0.933

3.936 ± 0.821

5.532 ± 0.763

4.149 ± 0.451

6.17 ± 1.26

9.043 ± 1.338

7.447 ± 1.625

6.489 ± 0.441

2.128 ± 0.611

4.043 ± 0.944
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski