Clupea harengus (Atlantic herring)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Otomorpha; Clupei; Clupeiformes; Clupeo

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P8H6S0|A0A6P8H6S0_CLUHA Isoform of A0A6P8GV27 protocadherin gamma-A11-like isoform X18 OS=Clupea harengus OX=7950 GN=LOC105912957 PE=4 SV=1
MM1 pKa = 8.05DD2 pKa = 5.03SCDD5 pKa = 3.33QCVSLSRR12 pKa = 11.84NLTPCVWRR20 pKa = 11.84ICDD23 pKa = 3.45NDD25 pKa = 3.63NNTGCVSDD33 pKa = 4.17TEE35 pKa = 5.32DD36 pKa = 3.86FQDD39 pKa = 4.01CNRR42 pKa = 11.84LNEE45 pKa = 4.07TDD47 pKa = 3.3MCAGTDD53 pKa = 3.11IDD55 pKa = 4.3IDD57 pKa = 3.42IDD59 pKa = 3.34IDD61 pKa = 3.62IDD63 pKa = 3.99AKK65 pKa = 10.39PAPAPEE71 pKa = 4.46LSQASFDD78 pKa = 3.62LSSFIGGIILVLVLQAGGFFAMRR101 pKa = 11.84FLKK104 pKa = 10.09TKK106 pKa = 10.28DD107 pKa = 3.17GTYY110 pKa = 8.95EE111 pKa = 4.15TIEE114 pKa = 4.04QPQQ117 pKa = 2.97

Molecular weight: 12.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P3VET6|A0A6P3VET6_CLUHA transmembrane gamma-carboxyglutamic acid protein 4 OS=Clupea harengus OX=7950 GN=prrg4 PE=4 SV=1
AA1 pKa = 7.66RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84SSSRR9 pKa = 11.84PIRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84PRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84TTRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84AGRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 3.5

Molecular weight: 4.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23855

13418

37273

27779145

30

30901

745.3

82.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.845 ± 0.011

2.047 ± 0.01

5.29 ± 0.009

7.273 ± 0.021

3.265 ± 0.009

6.588 ± 0.02

2.594 ± 0.009

4.168 ± 0.016

5.703 ± 0.023

9.032 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.388 ± 0.007

3.618 ± 0.007

6.141 ± 0.019

4.815 ± 0.019

5.698 ± 0.011

8.669 ± 0.016

5.808 ± 0.018

6.358 ± 0.018

1.092 ± 0.005

2.605 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski