Nitrospirillum amazonense CBAmc

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Azospirillaceae; Nitrospirillum; Nitrospirillum amazonense

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6034 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A248K1B3|A0A248K1B3_9PROT Chemotaxis protein OS=Nitrospirillum amazonense CBAmc OX=1441467 GN=Y958_22900 PE=4 SV=1
MM1 pKa = 7.56SSASDD6 pKa = 3.57SPTDD10 pKa = 3.64DD11 pKa = 4.05GEE13 pKa = 4.39IVSVDD18 pKa = 3.25TSDD21 pKa = 3.41PTVACLSIVSWSMAIAALGAIVLLALVLMLLNVSQYY57 pKa = 9.98VFLMVWIIGAPFAAAVAALGVLFIQLNQRR86 pKa = 11.84SQHH89 pKa = 4.62GG90 pKa = 3.46

Molecular weight: 9.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A248JM57|A0A248JM57_9PROT Vitamin B12-dependent ribonucleotide reductase OS=Nitrospirillum amazonense CBAmc OX=1441467 GN=Y958_02350 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.58LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84KK29 pKa = 8.81VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.16RR41 pKa = 11.84LSAA44 pKa = 3.93

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6034

0

6034

2143625

26

9845

355.3

38.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.472 ± 0.05

0.742 ± 0.011

5.845 ± 0.035

4.636 ± 0.047

3.194 ± 0.025

9.303 ± 0.083

2.092 ± 0.021

4.198 ± 0.026

2.617 ± 0.03

10.625 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.209 ± 0.021

2.571 ± 0.047

5.566 ± 0.052

3.386 ± 0.027

6.992 ± 0.064

5.014 ± 0.065

6.085 ± 0.09

7.752 ± 0.026

1.377 ± 0.016

2.325 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski