Amino acid dipepetide frequency for Nitrospirillum amazonense CBAmc

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.199AlaAla: 20.199 ± 0.192	1.115AlaCys: 1.115 ± 0.029	7.864AlaAsp: 7.864 ± 0.083	6.851AlaGlu: 6.851 ± 0.1	3.981AlaPhe: 3.981 ± 0.057	12.82AlaGly: 12.82 ± 0.163	2.517AlaHis: 2.517 ± 0.045	5.323AlaIle: 5.323 ± 0.057	3.783AlaLys: 3.783 ± 0.063	15.178AlaLeu: 15.178 ± 0.151	3.085AlaMet: 3.085 ± 0.047	3.148AlaAsn: 3.148 ± 0.092	6.674AlaPro: 6.674 ± 0.087	4.863AlaGln: 4.863 ± 0.097	9.039AlaArg: 9.039 ± 0.131	6.121AlaSer: 6.121 ± 0.139	7.38AlaThr: 7.38 ± 0.117	10.087AlaVal: 10.087 ± 0.08	1.845AlaTrp: 1.845 ± 0.035	2.846AlaTyr: 2.846 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.918CysAla: 0.918 ± 0.023	0.104CysCys: 0.104 ± 0.007	0.438CysAsp: 0.438 ± 0.014	0.279CysGlu: 0.279 ± 0.012	0.266CysPhe: 0.266 ± 0.013	0.812CysGly: 0.812 ± 0.023	0.228CysHis: 0.228 ± 0.012	0.282CysIle: 0.282 ± 0.012	0.131CysLys: 0.131 ± 0.008	0.841CysLeu: 0.841 ± 0.025	0.12CysMet: 0.12 ± 0.008	0.161CysAsn: 0.161 ± 0.01	0.476CysPro: 0.476 ± 0.015	0.226CysGln: 0.226 ± 0.011	0.603CysArg: 0.603 ± 0.017	0.333CysSer: 0.333 ± 0.012	0.39CysThr: 0.39 ± 0.015	0.527CysVal: 0.527 ± 0.018	0.117CysTrp: 0.117 ± 0.008	0.167CysTyr: 0.167 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.352AspAla: 7.352 ± 0.089	0.415AspCys: 0.415 ± 0.015	3.106AspAsp: 3.106 ± 0.054	2.736AspGlu: 2.736 ± 0.048	2.008AspPhe: 2.008 ± 0.034	5.9AspGly: 5.9 ± 0.068	1.348AspHis: 1.348 ± 0.027	2.768AspIle: 2.768 ± 0.042	1.465AspLys: 1.465 ± 0.037	6.632AspLeu: 6.632 ± 0.067	1.313AspMet: 1.313 ± 0.028	1.288AspAsn: 1.288 ± 0.031	3.429AspPro: 3.429 ± 0.059	2.147AspGln: 2.147 ± 0.059	4.387AspArg: 4.387 ± 0.071	2.202AspSer: 2.202 ± 0.037	3.051AspThr: 3.051 ± 0.056	4.142AspVal: 4.142 ± 0.058	1.088AspTrp: 1.088 ± 0.027	1.67AspTyr: 1.67 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	6.761GluAla: 6.761 ± 0.094	0.246GluCys: 0.246 ± 0.012	2.351GluAsp: 2.351 ± 0.045	2.676GluGlu: 2.676 ± 0.048	1.325GluPhe: 1.325 ± 0.032	3.963GluGly: 3.963 ± 0.056	0.943GluHis: 0.943 ± 0.025	2.332GluIle: 2.332 ± 0.043	1.573GluLys: 1.573 ± 0.037	4.233GluLeu: 4.233 ± 0.055	1.226GluMet: 1.226 ± 0.027	1.12GluAsn: 1.12 ± 0.032	2.176GluPro: 2.176 ± 0.042	1.777GluGln: 1.777 ± 0.037	4.0GluArg: 4.0 ± 0.067	1.721GluSer: 1.721 ± 0.031	2.826GluThr: 2.826 ± 0.05	3.718GluVal: 3.718 ± 0.058	0.561GluTrp: 0.561 ± 0.018	0.831GluTyr: 0.831 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	3.709PheAla: 3.709 ± 0.048	0.296PheCys: 0.296 ± 0.012	2.273PheAsp: 2.273 ± 0.039	1.435PheGlu: 1.435 ± 0.032	1.144PhePhe: 1.144 ± 0.028	3.089PheGly: 3.089 ± 0.045	0.763PheHis: 0.763 ± 0.021	1.375PheIle: 1.375 ± 0.029	0.833PheLys: 0.833 ± 0.021	3.15PheLeu: 3.15 ± 0.053	0.689PheMet: 0.689 ± 0.023	1.032PheAsn: 1.032 ± 0.025	1.544PhePro: 1.544 ± 0.032	1.111PheGln: 1.111 ± 0.024	2.06PheArg: 2.06 ± 0.041	1.815PheSer: 1.815 ± 0.035	2.111PheThr: 2.111 ± 0.033	2.207PheVal: 2.207 ± 0.037	0.469PheTrp: 0.469 ± 0.016	0.834PheTyr: 0.834 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	10.726GlyAla: 10.726 ± 0.176	0.781GlyCys: 0.781 ± 0.022	4.946GlyAsp: 4.946 ± 0.058	3.912GlyGlu: 3.912 ± 0.062	3.277GlyPhe: 3.277 ± 0.047	9.19GlyGly: 9.19 ± 0.237	2.119GlyHis: 2.119 ± 0.036	4.003GlyIle: 4.003 ± 0.045	2.662GlyLys: 2.662 ± 0.042	9.848GlyLeu: 9.848 ± 0.104	2.21GlyMet: 2.21 ± 0.031	2.418GlyAsn: 2.418 ± 0.121	4.191GlyPro: 4.191 ± 0.064	3.46GlyGln: 3.46 ± 0.043	6.712GlyArg: 6.712 ± 0.1	4.74GlySer: 4.74 ± 0.189	6.775GlyThr: 6.775 ± 0.417	7.053GlyVal: 7.053 ± 0.096	1.496GlyTrp: 1.496 ± 0.033	2.51GlyTyr: 2.51 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.481HisAla: 2.481 ± 0.041	0.212HisCys: 0.212 ± 0.009	1.269HisAsp: 1.269 ± 0.028	0.917HisGlu: 0.917 ± 0.023	0.775HisPhe: 0.775 ± 0.023	2.036HisGly: 2.036 ± 0.041	0.652HisHis: 0.652 ± 0.023	0.884HisIle: 0.884 ± 0.018	0.425HisLys: 0.425 ± 0.016	2.297HisLeu: 2.297 ± 0.04	0.479HisMet: 0.479 ± 0.015	0.459HisAsn: 0.459 ± 0.015	1.49HisPro: 1.49 ± 0.03	0.703HisGln: 0.703 ± 0.02	1.485HisArg: 1.485 ± 0.036	0.845HisSer: 0.845 ± 0.022	1.018HisThr: 1.018 ± 0.023	1.528HisVal: 1.528 ± 0.028	0.369HisTrp: 0.369 ± 0.015	0.591HisTyr: 0.591 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	5.615IleAla: 5.615 ± 0.065	0.33IleCys: 0.33 ± 0.014	2.896IleAsp: 2.896 ± 0.042	2.176IleGlu: 2.176 ± 0.037	1.123IlePhe: 1.123 ± 0.025	4.163IleGly: 4.163 ± 0.095	0.889IleHis: 0.889 ± 0.02	1.654IleIle: 1.654 ± 0.035	1.028IleLys: 1.028 ± 0.024	3.928IleLeu: 3.928 ± 0.051	0.736IleMet: 0.736 ± 0.019	1.324IleAsn: 1.324 ± 0.044	2.145IlePro: 2.145 ± 0.04	1.371IleGln: 1.371 ± 0.033	2.738IleArg: 2.738 ± 0.041	2.456IleSer: 2.456 ± 0.067	2.871IleThr: 2.871 ± 0.082	3.174IleVal: 3.174 ± 0.045	0.433IleTrp: 0.433 ± 0.017	0.93IleTyr: 0.93 ± 0.024	0.0IleXaa: 0.0 ± 0.0
Lys	3.997LysAla: 3.997 ± 0.068	0.107LysCys: 0.107 ± 0.007	1.508LysAsp: 1.508 ± 0.036	1.184LysGlu: 1.184 ± 0.032	0.658LysPhe: 0.658 ± 0.02	2.427LysGly: 2.427 ± 0.044	0.427LysHis: 0.427 ± 0.015	1.103LysIle: 1.103 ± 0.027	0.799LysLys: 0.799 ± 0.025	2.568LysLeu: 2.568 ± 0.046	0.554LysMet: 0.554 ± 0.015	0.593LysAsn: 0.593 ± 0.019	1.793LysPro: 1.793 ± 0.031	0.758LysGln: 0.758 ± 0.019	1.835LysArg: 1.835 ± 0.036	1.295LysSer: 1.295 ± 0.028	1.528LysThr: 1.528 ± 0.032	2.257LysVal: 2.257 ± 0.045	0.266LysTrp: 0.266 ± 0.012	0.517LysTyr: 0.517 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	15.953LeuAla: 15.953 ± 0.127	0.841LeuCys: 0.841 ± 0.023	6.552LeuAsp: 6.552 ± 0.076	4.733LeuGlu: 4.733 ± 0.063	3.399LeuPhe: 3.399 ± 0.057	8.968LeuGly: 8.968 ± 0.089	1.996LeuHis: 1.996 ± 0.037	4.038LeuIle: 4.038 ± 0.052	3.113LeuLys: 3.113 ± 0.051	10.107LeuLeu: 10.107 ± 0.102	2.371LeuMet: 2.371 ± 0.039	2.884LeuAsn: 2.884 ± 0.081	6.228LeuPro: 6.228 ± 0.1	2.605LeuGln: 2.605 ± 0.036	7.123LeuArg: 7.123 ± 0.091	6.591LeuSer: 6.591 ± 0.142	7.023LeuThr: 7.023 ± 0.189	8.237LeuVal: 8.237 ± 0.097	1.229LeuTrp: 1.229 ± 0.031	2.26LeuTyr: 2.26 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	3.365MetAla: 3.365 ± 0.045	0.111MetCys: 0.111 ± 0.008	1.275MetAsp: 1.275 ± 0.027	1.008MetGlu: 1.008 ± 0.022	0.487MetPhe: 0.487 ± 0.014	1.947MetGly: 1.947 ± 0.034	0.363MetHis: 0.363 ± 0.012	0.915MetIle: 0.915 ± 0.023	0.686MetLys: 0.686 ± 0.02	2.025MetLeu: 2.025 ± 0.035	0.564MetMet: 0.564 ± 0.018	0.557MetAsn: 0.557 ± 0.017	1.449MetPro: 1.449 ± 0.031	0.606MetGln: 0.606 ± 0.016	1.587MetArg: 1.587 ± 0.038	1.295MetSer: 1.295 ± 0.026	1.69MetThr: 1.69 ± 0.036	1.738MetVal: 1.738 ± 0.03	0.161MetTrp: 0.161 ± 0.009	0.263MetTyr: 0.263 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	3.167AsnAla: 3.167 ± 0.083	0.21AsnCys: 0.21 ± 0.011	1.453AsnAsp: 1.453 ± 0.055	0.926AsnGlu: 0.926 ± 0.021	0.823AsnPhe: 0.823 ± 0.021	2.572AsnGly: 2.572 ± 0.086	0.528AsnHis: 0.528 ± 0.018	1.143AsnIle: 1.143 ± 0.031	0.523AsnLys: 0.523 ± 0.019	2.821AsnLeu: 2.821 ± 0.074	0.468AsnMet: 0.468 ± 0.014	0.807AsnAsn: 0.807 ± 0.047	1.709AsnPro: 1.709 ± 0.035	0.911AsnGln: 0.911 ± 0.025	1.708AsnArg: 1.708 ± 0.031	1.442AsnSer: 1.442 ± 0.116	1.511AsnThr: 1.511 ± 0.081	1.854AsnVal: 1.854 ± 0.062	0.358AsnTrp: 0.358 ± 0.014	0.78AsnTyr: 0.78 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	7.973ProAla: 7.973 ± 0.114	0.333ProCys: 0.333 ± 0.013	4.002ProAsp: 4.002 ± 0.07	3.104ProGlu: 3.104 ± 0.055	1.78ProPhe: 1.78 ± 0.034	5.257ProGly: 5.257 ± 0.066	1.109ProHis: 1.109 ± 0.029	1.93ProIle: 1.93 ± 0.036	1.448ProLys: 1.448 ± 0.036	5.612ProLeu: 5.612 ± 0.085	1.201ProMet: 1.201 ± 0.025	1.258ProAsn: 1.258 ± 0.029	3.486ProPro: 3.486 ± 0.078	1.811ProGln: 1.811 ± 0.036	3.032ProArg: 3.032 ± 0.054	2.697ProSer: 2.697 ± 0.043	2.961ProThr: 2.961 ± 0.039	4.591ProVal: 4.591 ± 0.064	0.806ProTrp: 0.806 ± 0.022	1.267ProTyr: 1.267 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	5.369GlnAla: 5.369 ± 0.071	0.174GlnCys: 0.174 ± 0.01	1.743GlnAsp: 1.743 ± 0.034	1.407GlnGlu: 1.407 ± 0.03	0.982GlnPhe: 0.982 ± 0.025	3.087GlnGly: 3.087 ± 0.042	0.634GlnHis: 0.634 ± 0.018	1.416GlnIle: 1.416 ± 0.042	0.797GlnLys: 0.797 ± 0.023	3.119GlnLeu: 3.119 ± 0.091	0.827GlnMet: 0.827 ± 0.023	0.79GlnAsn: 0.79 ± 0.021	2.118GlnPro: 2.118 ± 0.043	1.226GlnGln: 1.226 ± 0.032	2.455GlnArg: 2.455 ± 0.044	1.72GlnSer: 1.72 ± 0.038	1.909GlnThr: 1.909 ± 0.037	3.007GlnVal: 3.007 ± 0.112	0.409GlnTrp: 0.409 ± 0.015	0.669GlnTyr: 0.669 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	8.372ArgAla: 8.372 ± 0.115	0.497ArgCys: 0.497 ± 0.015	4.094ArgAsp: 4.094 ± 0.066	3.295ArgGlu: 3.295 ± 0.059	2.603ArgPhe: 2.603 ± 0.045	4.881ArgGly: 4.881 ± 0.065	1.959ArgHis: 1.959 ± 0.043	3.106ArgIle: 3.106 ± 0.049	1.624ArgLys: 1.624 ± 0.037	8.829ArgLeu: 8.829 ± 0.122	1.631ArgMet: 1.631 ± 0.034	1.548ArgAsn: 1.548 ± 0.025	3.911ArgPro: 3.911 ± 0.062	2.953ArgGln: 2.953 ± 0.057	6.162ArgArg: 6.162 ± 0.101	2.896ArgSer: 2.896 ± 0.051	3.556ArgThr: 3.556 ± 0.049	4.979ArgVal: 4.979 ± 0.069	1.18ArgTrp: 1.18 ± 0.03	1.838ArgTyr: 1.838 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	6.051SerAla: 6.051 ± 0.113	0.362SerCys: 0.362 ± 0.014	2.603SerAsp: 2.603 ± 0.047	1.731SerGlu: 1.731 ± 0.036	1.794SerPhe: 1.794 ± 0.031	5.706SerGly: 5.706 ± 0.274	1.049SerHis: 1.049 ± 0.023	2.203SerIle: 2.203 ± 0.078	1.084SerLys: 1.084 ± 0.023	5.625SerLeu: 5.625 ± 0.087	1.037SerMet: 1.037 ± 0.026	1.435SerAsn: 1.435 ± 0.078	2.818SerPro: 2.818 ± 0.046	1.678SerGln: 1.678 ± 0.051	3.141SerArg: 3.141 ± 0.05	2.856SerSer: 2.856 ± 0.106	2.947SerThr: 2.947 ± 0.105	3.861SerVal: 3.861 ± 0.084	0.734SerTrp: 0.734 ± 0.019	1.428SerTyr: 1.428 ± 0.055	0.0SerXaa: 0.0 ± 0.0
Thr	7.882ThrAla: 7.882 ± 0.131	0.375ThrCys: 0.375 ± 0.013	3.195ThrAsp: 3.195 ± 0.065	2.333ThrGlu: 2.333 ± 0.041	1.897ThrPhe: 1.897 ± 0.035	6.378ThrGly: 6.378 ± 0.277	1.089ThrHis: 1.089 ± 0.025	2.909ThrIle: 2.909 ± 0.159	1.242ThrLys: 1.242 ± 0.03	7.645ThrLeu: 7.645 ± 0.24	1.042ThrMet: 1.042 ± 0.024	1.552ThrAsn: 1.552 ± 0.066	3.925ThrPro: 3.925 ± 0.048	1.823ThrGln: 1.823 ± 0.045	3.383ThrArg: 3.383 ± 0.05	2.897ThrSer: 2.897 ± 0.104	3.556ThrThr: 3.556 ± 0.191	5.583ThrVal: 5.583 ± 0.13	0.755ThrTrp: 0.755 ± 0.021	1.391ThrTyr: 1.391 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	10.617ValAla: 10.617 ± 0.088	0.566ValCys: 0.566 ± 0.018	4.658ValAsp: 4.658 ± 0.06	4.129ValGlu: 4.129 ± 0.063	2.305ValPhe: 2.305 ± 0.035	6.535ValGly: 6.535 ± 0.097	1.425ValHis: 1.425 ± 0.029	3.15ValIle: 3.15 ± 0.043	2.026ValLys: 2.026 ± 0.042	7.825ValLeu: 7.825 ± 0.079	1.786ValMet: 1.786 ± 0.029	2.269ValAsn: 2.269 ± 0.093	4.237ValPro: 4.237 ± 0.063	2.353ValGln: 2.353 ± 0.049	5.243ValArg: 5.243 ± 0.067	4.17ValSer: 4.17 ± 0.098	5.437ValThr: 5.437 ± 0.116	6.369ValVal: 6.369 ± 0.075	0.926ValTrp: 0.926 ± 0.02	1.492ValTyr: 1.492 ± 0.025	0.0ValXaa: 0.0 ± 0.0
Trp	1.46TrpAla: 1.46 ± 0.034	0.129TrpCys: 0.129 ± 0.008	0.707TrpAsp: 0.707 ± 0.021	0.501TrpGlu: 0.501 ± 0.017	0.479TrpPhe: 0.479 ± 0.016	1.03TrpGly: 1.03 ± 0.024	0.369TrpHis: 0.369 ± 0.015	0.532TrpIle: 0.532 ± 0.018	0.362TrpLys: 0.362 ± 0.013	1.669TrpLeu: 1.669 ± 0.037	0.317TrpMet: 0.317 ± 0.015	0.418TrpAsn: 0.418 ± 0.016	0.737TrpPro: 0.737 ± 0.022	0.606TrpGln: 0.606 ± 0.018	1.344TrpArg: 1.344 ± 0.031	0.759TrpSer: 0.759 ± 0.018	0.877TrpThr: 0.877 ± 0.023	0.931TrpVal: 0.931 ± 0.022	0.228TrpTrp: 0.228 ± 0.011	0.316TrpTyr: 0.316 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.755TyrAla: 2.755 ± 0.043	0.215TyrCys: 0.215 ± 0.01	1.513TyrAsp: 1.513 ± 0.035	1.024TyrGlu: 1.024 ± 0.024	0.836TyrPhe: 0.836 ± 0.023	2.269TyrGly: 2.269 ± 0.038	0.508TyrHis: 0.508 ± 0.018	0.911TyrIle: 0.911 ± 0.03	0.568TyrLys: 0.568 ± 0.018	2.3TyrLeu: 2.3 ± 0.037	0.432TyrMet: 0.432 ± 0.014	0.651TyrAsn: 0.651 ± 0.023	1.121TyrPro: 1.121 ± 0.026	0.872TyrGln: 0.872 ± 0.023	1.878TyrArg: 1.878 ± 0.039	1.291TyrSer: 1.291 ± 0.055	1.436TyrThr: 1.436 ± 0.039	1.674TyrVal: 1.674 ± 0.029	0.345TyrTrp: 0.345 ± 0.013	0.651TyrTyr: 0.651 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6034 proteins (2143625 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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