Salegentibacter flavus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Salegentibacter

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3319 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4Z736|A0A1I4Z736_9FLAO Predicted N-formylglutamate amidohydrolase OS=Salegentibacter flavus OX=287099 GN=SAMN05660413_01154 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.38NILKK6 pKa = 10.24ISLSVFMLVFFMACTDD22 pKa = 3.61DD23 pKa = 5.77DD24 pKa = 4.76YY25 pKa = 11.76DD26 pKa = 3.91IGEE29 pKa = 4.16ITAPTNLQVEE39 pKa = 4.54TAILGQSDD47 pKa = 4.55DD48 pKa = 4.26MPDD51 pKa = 3.44GDD53 pKa = 4.43GSGEE57 pKa = 4.15VTFTATAQGAMTYY70 pKa = 10.9KK71 pKa = 10.84FIFEE75 pKa = 4.64NGTEE79 pKa = 4.12VTTASGVYY87 pKa = 7.04THH89 pKa = 7.43PFSEE93 pKa = 4.82TGTQTYY99 pKa = 9.51NVNIIAYY106 pKa = 8.72GPGGTASSTSVEE118 pKa = 4.22VAVLVTYY125 pKa = 7.85EE126 pKa = 4.22PPADD130 pKa = 4.04LLEE133 pKa = 4.22KK134 pKa = 10.8LIGDD138 pKa = 4.16GAKK141 pKa = 9.31EE142 pKa = 3.75WRR144 pKa = 11.84IKK146 pKa = 11.01AEE148 pKa = 3.97AAGHH152 pKa = 6.22FGLGPVGGSIPTEE165 pKa = 4.0WYY167 pKa = 10.16GAGPNEE173 pKa = 4.18KK174 pKa = 9.78AHH176 pKa = 6.14TGMYY180 pKa = 9.3DD181 pKa = 3.04DD182 pKa = 5.6RR183 pKa = 11.84YY184 pKa = 10.48IFEE187 pKa = 4.31EE188 pKa = 4.95DD189 pKa = 3.29GTFVHH194 pKa = 6.49ITDD197 pKa = 3.9ITNDD201 pKa = 3.81DD202 pKa = 3.27ASGTVFGRR210 pKa = 11.84APLVEE215 pKa = 4.07QLGVSCDD222 pKa = 3.83CEE224 pKa = 4.46TEE226 pKa = 4.11GDD228 pKa = 3.88DD229 pKa = 5.18VLNIPYY235 pKa = 10.26DD236 pKa = 4.03DD237 pKa = 3.93YY238 pKa = 11.6TEE240 pKa = 4.02NWSISAPGGNEE251 pKa = 4.06TINLTGIGFIGYY263 pKa = 9.72YY264 pKa = 9.54IGGDD268 pKa = 3.15HH269 pKa = 7.24RR270 pKa = 11.84YY271 pKa = 9.84EE272 pKa = 3.93IFDD275 pKa = 3.29RR276 pKa = 11.84SVPNEE281 pKa = 4.35LIIKK285 pKa = 8.38STDD288 pKa = 3.03GNGDD292 pKa = 3.55FDD294 pKa = 3.22WWFILTSDD302 pKa = 3.63SGGGAEE308 pKa = 4.18EE309 pKa = 4.5EE310 pKa = 4.43FVSEE314 pKa = 4.0YY315 pKa = 11.28NEE317 pKa = 3.69LHH319 pKa = 6.88RR320 pKa = 11.84EE321 pKa = 4.06FNFDD325 pKa = 3.25TDD327 pKa = 4.12GEE329 pKa = 4.63LDD331 pKa = 3.48TTVWNFEE338 pKa = 4.24TGNGEE343 pKa = 4.44DD344 pKa = 3.07GWGNQEE350 pKa = 3.79SQYY353 pKa = 9.2YY354 pKa = 8.98TEE356 pKa = 5.07DD357 pKa = 3.29NAVISGGNLVITAKK371 pKa = 10.94AEE373 pKa = 4.6DD374 pKa = 3.96IEE376 pKa = 4.68GFDD379 pKa = 3.92YY380 pKa = 10.93SSSRR384 pKa = 11.84ITTKK388 pKa = 11.12DD389 pKa = 3.0NFEE392 pKa = 3.94FTYY395 pKa = 10.88GRR397 pKa = 11.84IEE399 pKa = 4.03ARR401 pKa = 11.84AKK403 pKa = 10.46LPEE406 pKa = 4.56GAGTWPAIWMLGSDD420 pKa = 4.11FDD422 pKa = 4.48EE423 pKa = 4.92VGWPQTGEE431 pKa = 3.59IDD433 pKa = 3.93IMEE436 pKa = 4.59HH437 pKa = 6.73AGNEE441 pKa = 3.57QDD443 pKa = 5.22LIHH446 pKa = 6.44GTLHH450 pKa = 5.72YY451 pKa = 9.82EE452 pKa = 4.17GRR454 pKa = 11.84SGGDD458 pKa = 2.85ADD460 pKa = 4.22GNTIEE465 pKa = 4.37VDD467 pKa = 3.65GVSDD471 pKa = 4.09DD472 pKa = 3.57FHH474 pKa = 8.72IYY476 pKa = 7.95TVEE479 pKa = 3.65WSDD482 pKa = 3.12EE483 pKa = 4.32HH484 pKa = 9.22IIFLVDD490 pKa = 3.15GEE492 pKa = 4.59VFHH495 pKa = 6.89TYY497 pKa = 10.32EE498 pKa = 4.69NNPDD502 pKa = 3.39SPFNKK507 pKa = 10.16DD508 pKa = 3.31FFLILNVAMGGTFGGEE524 pKa = 3.72INPDD528 pKa = 3.47FVEE531 pKa = 4.47SSMEE535 pKa = 3.39VDD537 pKa = 4.17YY538 pKa = 11.42IRR540 pKa = 11.84VFQQ543 pKa = 4.13

Molecular weight: 59.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4YP95|A0A1I4YP95_9FLAO Ribonucleoside-diphosphate reductase alpha chain OS=Salegentibacter flavus OX=287099 GN=SAMN05660413_00884 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.2RR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 9.04VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.73LSVSSEE47 pKa = 3.76NRR49 pKa = 11.84HH50 pKa = 4.47KK51 pKa = 10.5HH52 pKa = 4.49

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3319

0

3319

1093900

40

3354

329.6

37.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.486 ± 0.033

0.642 ± 0.013

5.527 ± 0.033

7.858 ± 0.052

5.176 ± 0.032

6.575 ± 0.037

1.786 ± 0.021

7.623 ± 0.038

7.502 ± 0.057

9.594 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.29 ± 0.021

5.532 ± 0.035

3.6 ± 0.021

3.418 ± 0.025

4.01 ± 0.03

6.292 ± 0.032

5.099 ± 0.035

6.0 ± 0.031

1.067 ± 0.017

3.923 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski