Aphanomyces euteiches

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Saprolegniales; Saprolegniaceae; Aphanomyces

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20021 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G0XMT3|A0A6G0XMT3_9STRA Ubiquitin-like domain-containing protein OS=Aphanomyces euteiches OX=100861 GN=Ae201684_003290 PE=4 SV=1
MM1 pKa = 7.3NALHH5 pKa = 6.99LADD8 pKa = 4.71CASNGAPVLIDD19 pKa = 4.11DD20 pKa = 4.69EE21 pKa = 5.18EE22 pKa = 4.74ILASFTNVRR31 pKa = 11.84MFTNTYY37 pKa = 10.55GPGEE41 pKa = 4.59DD42 pKa = 4.9DD43 pKa = 4.81SPVMDD48 pKa = 4.72AKK50 pKa = 11.15GSGNLYY56 pKa = 8.12VTTSRR61 pKa = 11.84VVWVFSQNGSGVVGYY76 pKa = 10.05AWDD79 pKa = 3.51MTYY82 pKa = 11.29LSLHH86 pKa = 6.72AISRR90 pKa = 11.84DD91 pKa = 3.08LSSFPEE97 pKa = 4.17PCLYY101 pKa = 10.58CQLDD105 pKa = 3.64VEE107 pKa = 5.1DD108 pKa = 4.64EE109 pKa = 4.29VNEE112 pKa = 4.0IRR114 pKa = 11.84FVPQDD119 pKa = 3.68PEE121 pKa = 4.04KK122 pKa = 10.6QLQALFDD129 pKa = 4.23AFSEE133 pKa = 4.69SAALNPDD140 pKa = 3.65DD141 pKa = 6.66DD142 pKa = 5.61EE143 pKa = 7.9DD144 pKa = 6.04DD145 pKa = 3.99EE146 pKa = 4.57QQGGDD151 pKa = 3.16WIYY154 pKa = 11.4NEE156 pKa = 4.74EE157 pKa = 4.18EE158 pKa = 4.24VSTGARR164 pKa = 11.84EE165 pKa = 4.0AALAAHH171 pKa = 7.19FDD173 pKa = 4.22SILQVAPSLQQHH185 pKa = 7.0ADD187 pKa = 3.46GQFDD191 pKa = 4.45DD192 pKa = 4.89ADD194 pKa = 4.02EE195 pKa = 6.53DD196 pKa = 3.95EE197 pKa = 4.56MLL199 pKa = 4.82

Molecular weight: 21.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G0W7F6|A0A6G0W7F6_9STRA Uncharacterized protein OS=Aphanomyces euteiches OX=100861 GN=Ae201684_018062 PE=4 SV=1
MM1 pKa = 7.57GFQLAFSNFLLSMRR15 pKa = 11.84VFLQLFLAAAAAALSHH31 pKa = 6.15GQLANQVVSFEE42 pKa = 4.29VSRR45 pKa = 11.84SLSATAGYY53 pKa = 9.42GALDD57 pKa = 3.64KK58 pKa = 10.52TVQTAAIHH66 pKa = 6.54GEE68 pKa = 3.98DD69 pKa = 3.96TEE71 pKa = 4.52TLRR74 pKa = 11.84SLRR77 pKa = 11.84RR78 pKa = 11.84KK79 pKa = 9.38GRR81 pKa = 11.84GRR83 pKa = 11.84RR84 pKa = 11.84GAKK87 pKa = 9.11SGRR90 pKa = 11.84RR91 pKa = 11.84RR92 pKa = 11.84NRR94 pKa = 11.84RR95 pKa = 11.84RR96 pKa = 11.84RR97 pKa = 11.84SRR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 11.84HH102 pKa = 5.27LMTVQSRR109 pKa = 11.84EE110 pKa = 3.84LAATAGGFMNGLKK123 pKa = 10.85SMGRR127 pKa = 11.84KK128 pKa = 8.94ISSAAGKK135 pKa = 9.85VGRR138 pKa = 11.84AASSATGKK146 pKa = 10.41LSRR149 pKa = 11.84ATSQVGQVAGQVGRR163 pKa = 11.84VAGKK167 pKa = 9.67VSRR170 pKa = 11.84TANRR174 pKa = 11.84VGRR177 pKa = 11.84VAGHH181 pKa = 5.91VSQVGGRR188 pKa = 11.84VGRR191 pKa = 11.84VAGNVGRR198 pKa = 11.84VAGQVRR204 pKa = 11.84RR205 pKa = 11.84GAGQVRR211 pKa = 11.84RR212 pKa = 11.84TAGQVGHH219 pKa = 6.24SARR222 pKa = 11.84QAGRR226 pKa = 11.84AGRR229 pKa = 11.84PTGGRR234 pKa = 11.84SSGGRR239 pKa = 11.84ASGRR243 pKa = 11.84PAGGRR248 pKa = 11.84GRR250 pKa = 11.84ASGSRR255 pKa = 11.84ATGGRR260 pKa = 11.84RR261 pKa = 11.84AAGGRR266 pKa = 11.84ASGGRR271 pKa = 11.84PARR274 pKa = 11.84RR275 pKa = 11.84KK276 pKa = 9.88AGRR279 pKa = 11.84ALRR282 pKa = 11.84GVGSNN287 pKa = 3.48

Molecular weight: 29.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19021

1000

20021

9106444

33

10036

454.8

50.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.621 ± 0.02

1.794 ± 0.01

5.657 ± 0.013

5.734 ± 0.019

3.964 ± 0.011

5.427 ± 0.019

2.754 ± 0.009

4.811 ± 0.01

5.208 ± 0.018

9.662 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.008

3.744 ± 0.009

5.107 ± 0.017

4.289 ± 0.013

5.534 ± 0.015

7.764 ± 0.019

6.164 ± 0.015

7.083 ± 0.015

1.394 ± 0.006

2.748 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski