Gordonia polyisoprenivorans (strain DSM 44266 / VH2)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Gordoniaceae; Gordonia; Gordonia polyisoprenivorans

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5097 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H6MU12|H6MU12_GORPV Putative polyketide synthase OS=Gordonia polyisoprenivorans (strain DSM 44266 / VH2) OX=1112204 GN=GPOL_c04180 PE=4 SV=1
MM1 pKa = 7.52SRR3 pKa = 11.84VHH5 pKa = 6.58LNGSTTLHH13 pKa = 6.97DD14 pKa = 3.99GGFPYY19 pKa = 10.61SSTVSPGPLLFTAGISPLDD38 pKa = 3.63EE39 pKa = 4.41NGAVIEE45 pKa = 4.73PGDD48 pKa = 3.46VVAQVRR54 pKa = 11.84ACLGNLAAVLGEE66 pKa = 4.04QGATFGDD73 pKa = 4.14VAKK76 pKa = 9.4LTVYY80 pKa = 10.24VAEE83 pKa = 4.63HH84 pKa = 6.17LQADD88 pKa = 5.14LYY90 pKa = 9.84VAWEE94 pKa = 3.98AVSAEE99 pKa = 4.05FDD101 pKa = 4.04VNPPTMMMGVSVLPYY116 pKa = 9.45DD117 pKa = 3.99GQVVEE122 pKa = 4.66VDD124 pKa = 3.91AVAAPPQSS132 pKa = 3.27

Molecular weight: 13.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6N387|H6N387_GORPV FAD dependent oxidoreductase OS=Gordonia polyisoprenivorans (strain DSM 44266 / VH2) OX=1112204 GN=GPOL_c00500 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVNSRR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.8GRR42 pKa = 11.84AKK44 pKa = 9.67LTAA47 pKa = 4.21

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5097

0

5097

1734384

37

13437

340.3

36.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.966 ± 0.044

0.766 ± 0.011

6.722 ± 0.032

5.058 ± 0.029

2.942 ± 0.018

8.903 ± 0.033

2.307 ± 0.015

4.592 ± 0.023

1.901 ± 0.026

9.531 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.007 ± 0.014

2.02 ± 0.022

5.686 ± 0.027

2.701 ± 0.019

7.4 ± 0.038

5.701 ± 0.024

6.545 ± 0.029

8.776 ± 0.036

1.455 ± 0.013

2.022 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski