Methanobacterium phage psiM2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Psimunavirus; Psimunavirus psiM2

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O80202|O80202_9CAUD Structural protein OS=Methanobacterium phage psiM2 OX=77048 PE=4 SV=1
MM1 pKa = 7.03VRR3 pKa = 11.84PGGVEE8 pKa = 3.77YY9 pKa = 10.76DD10 pKa = 3.75HH11 pKa = 6.97EE12 pKa = 6.61RR13 pKa = 11.84IDD15 pKa = 3.81YY16 pKa = 9.42MFDD19 pKa = 3.05EE20 pKa = 5.28FLADD24 pKa = 3.32IQDD27 pKa = 3.33VLTRR31 pKa = 11.84LKK33 pKa = 9.82VTDD36 pKa = 3.69VEE38 pKa = 4.77SFLSDD43 pKa = 3.07FHH45 pKa = 8.26LLTKK49 pKa = 10.6AEE51 pKa = 3.93FMRR54 pKa = 11.84RR55 pKa = 11.84YY56 pKa = 9.28GVSEE60 pKa = 3.96RR61 pKa = 11.84EE62 pKa = 4.1YY63 pKa = 11.49NEE65 pKa = 3.78LQAALQEE72 pKa = 4.35VEE74 pKa = 5.15DD75 pKa = 4.53LNLPTFEE82 pKa = 6.49DD83 pKa = 3.8EE84 pKa = 6.3DD85 pKa = 3.94LAQIIWFMGLAVVYY99 pKa = 9.07LARR102 pKa = 11.84DD103 pKa = 3.49IEE105 pKa = 4.64RR106 pKa = 11.84DD107 pKa = 3.63TEE109 pKa = 3.88IQAYY113 pKa = 7.86CQHH116 pKa = 6.4SEE118 pKa = 3.68AHH120 pKa = 6.17GGEE123 pKa = 5.01LYY125 pKa = 11.08LNWVTMGDD133 pKa = 3.49DD134 pKa = 4.37HH135 pKa = 9.24VCEE138 pKa = 4.25TCQDD142 pKa = 3.95YY143 pKa = 11.47EE144 pKa = 4.43DD145 pKa = 4.38GSPYY149 pKa = 10.01RR150 pKa = 11.84VEE152 pKa = 4.32EE153 pKa = 4.51FPHH156 pKa = 6.55IPHH159 pKa = 7.19PFCRR163 pKa = 11.84CHH165 pKa = 7.32PEE167 pKa = 4.03PCDD170 pKa = 3.55EE171 pKa = 4.33NGRR174 pKa = 11.84PIDD177 pKa = 4.44DD178 pKa = 3.87LSEE181 pKa = 4.12YY182 pKa = 10.88SGTEE186 pKa = 3.5LEE188 pKa = 4.67YY189 pKa = 11.0YY190 pKa = 10.59DD191 pKa = 5.85EE192 pKa = 4.59IMEE195 pKa = 4.64DD196 pKa = 3.7FGDD199 pKa = 3.64VGIDD203 pKa = 3.74FEE205 pKa = 5.23AVPPPPPYY213 pKa = 10.75YY214 pKa = 10.76YY215 pKa = 10.05II216 pKa = 4.91

Molecular weight: 25.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O80220|O80220_9CAUD Uncharacterized protein OS=Methanobacterium phage psiM2 OX=77048 PE=4 SV=1
MM1 pKa = 7.6IVIRR5 pKa = 11.84GKK7 pKa = 10.76GIYY10 pKa = 9.33RR11 pKa = 11.84CPLAGFGSRR20 pKa = 11.84IRR22 pKa = 11.84GVVYY26 pKa = 10.18EE27 pKa = 4.85RR28 pKa = 11.84EE29 pKa = 4.18APPLGGDD36 pKa = 3.06RR37 pKa = 11.84KK38 pKa = 10.47GGLRR42 pKa = 11.84EE43 pKa = 3.72IYY45 pKa = 10.05LISSRR50 pKa = 11.84YY51 pKa = 9.57FLFCSMKK58 pKa = 10.4RR59 pKa = 11.84SIASSQADD67 pKa = 3.48VMGVFSNLSIAIALNIFNFFSDD89 pKa = 3.65TLKK92 pKa = 10.08FTGTFFSSLSFMIVTHH108 pKa = 5.55VASHH112 pKa = 6.49KK113 pKa = 10.79KK114 pKa = 10.49LIFSINIIKK123 pKa = 10.29NN124 pKa = 3.29

Molecular weight: 13.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31

0

31

7532

90

1186

243.0

27.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.284 ± 0.274

0.611 ± 0.13

6.094 ± 0.404

8.71 ± 0.481

4.01 ± 0.253

6.718 ± 0.277

1.739 ± 0.238

6.691 ± 0.411

6.36 ± 0.386

8.617 ± 0.436

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.642 ± 0.234

4.156 ± 0.287

4.992 ± 0.428

2.244 ± 0.176

6.452 ± 0.432

6.413 ± 0.374

5.629 ± 0.265

6.944 ± 0.318

1.394 ± 0.204

4.302 ± 0.357
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski