Syntrophaceticus schinkii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacterales Family III. Incertae Sedis; Syntrophaceticus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3167 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B7MQ13|A0A0B7MQ13_9FIRM Hydrogenase expression/formation protein HypC OS=Syntrophaceticus schinkii OX=499207 GN=hypC PE=3 SV=1
MM1 pKa = 7.88DD2 pKa = 5.9LVPEE6 pKa = 5.28HH7 pKa = 6.97ISSAYY12 pKa = 9.24AGGSIEE18 pKa = 4.0HH19 pKa = 5.99VKK21 pKa = 10.54VSRR24 pKa = 11.84EE25 pKa = 3.99SGEE28 pKa = 4.07SGNNSEE34 pKa = 5.76LILIEE39 pKa = 3.82SWGTYY44 pKa = 8.57QEE46 pKa = 4.38ACCILQAMIRR56 pKa = 11.84VDD58 pKa = 3.44RR59 pKa = 11.84SLDD62 pKa = 3.49FGRR65 pKa = 11.84GLCISSPSEE74 pKa = 3.97KK75 pKa = 10.08PSVFSPGCRR84 pKa = 11.84IISEE88 pKa = 4.67LYY90 pKa = 8.84NTDD93 pKa = 2.9GALNISDD100 pKa = 3.76STVNGDD106 pKa = 3.18IYY108 pKa = 10.99TGGDD112 pKa = 3.17LTMSGDD118 pKa = 3.18THH120 pKa = 7.21LAGDD124 pKa = 3.84INGEE128 pKa = 4.35GMFTACPNCRR138 pKa = 11.84VVGNVQVTGDD148 pKa = 3.71VQVEE152 pKa = 4.05DD153 pKa = 4.28DD154 pKa = 4.23VVIEE158 pKa = 3.91GDD160 pKa = 2.89IRR162 pKa = 11.84AAGDD166 pKa = 3.4VYY168 pKa = 10.66IRR170 pKa = 11.84KK171 pKa = 9.03AAVSGSIWSNGEE183 pKa = 3.51IFVEE187 pKa = 4.19QGGQVEE193 pKa = 4.4GGIYY197 pKa = 9.68PGQAMEE203 pKa = 5.42LDD205 pKa = 3.51VALPFFPEE213 pKa = 3.39VDD215 pKa = 3.08LSFFRR220 pKa = 11.84RR221 pKa = 11.84GADD224 pKa = 3.31QILKK228 pKa = 8.66GTQQLHH234 pKa = 6.8GILHH238 pKa = 6.58FDD240 pKa = 2.91GVTFIEE246 pKa = 4.94GDD248 pKa = 3.57LEE250 pKa = 4.1IAGDD254 pKa = 3.9YY255 pKa = 8.25TGKK258 pKa = 10.95GILVVDD264 pKa = 4.44GSVGISGDD272 pKa = 3.86LLPVGIQDD280 pKa = 3.99SLCILAAGPVTCADD294 pKa = 4.44GSCLSLLVYY303 pKa = 10.63GKK305 pKa = 10.45DD306 pKa = 3.48DD307 pKa = 3.91LSLGEE312 pKa = 3.94GSRR315 pKa = 11.84FQGSITAYY323 pKa = 8.08TLRR326 pKa = 11.84MCNNAEE332 pKa = 4.15FIYY335 pKa = 11.01DD336 pKa = 3.74GDD338 pKa = 4.36LVDD341 pKa = 4.75

Molecular weight: 36.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B7MBM4|A0A0B7MBM4_9FIRM Uncharacterized protein OS=Syntrophaceticus schinkii OX=499207 GN=SSCH_1060007 PE=4 SV=1
MM1 pKa = 7.26TCLFAWNFGGIEE13 pKa = 3.91ITSLRR18 pKa = 11.84RR19 pKa = 11.84LDD21 pKa = 4.24VEE23 pKa = 4.29TQRR26 pKa = 11.84SQKK29 pKa = 10.53SLAEE33 pKa = 3.91IKK35 pKa = 9.65IWPARR40 pKa = 11.84EE41 pKa = 4.23FIYY44 pKa = 10.74HH45 pKa = 7.44PDD47 pKa = 3.17LAAPAVEE54 pKa = 5.39RR55 pKa = 11.84IQEE58 pKa = 4.16AYY60 pKa = 9.58KK61 pKa = 10.41KK62 pKa = 10.58RR63 pKa = 11.84RR64 pKa = 11.84DD65 pKa = 3.56ALKK68 pKa = 10.32GSRR71 pKa = 11.84NALTRR76 pKa = 11.84LQQRR80 pKa = 11.84ANRR83 pKa = 11.84FLEE86 pKa = 4.12MARR89 pKa = 11.84EE90 pKa = 4.31GVGRR94 pKa = 11.84SLNLVQPYY102 pKa = 8.75FYY104 pKa = 10.08PEE106 pKa = 3.92QVSLLDD112 pKa = 3.8YY113 pKa = 11.05LPADD117 pKa = 3.52SLVILDD123 pKa = 3.74EE124 pKa = 4.54PGRR127 pKa = 11.84FPEE130 pKa = 3.98QSRR133 pKa = 11.84ARR135 pKa = 11.84TVLLDD140 pKa = 3.17SDD142 pKa = 3.69YY143 pKa = 11.41RR144 pKa = 11.84RR145 pKa = 11.84FFQEE149 pKa = 4.36GNSFTPWQEE158 pKa = 3.82HH159 pKa = 5.79YY160 pKa = 11.04FDD162 pKa = 5.48GDD164 pKa = 3.49QLLKK168 pKa = 10.95QMAEE172 pKa = 4.19FPLICYY178 pKa = 9.93SRR180 pKa = 11.84LLTRR184 pKa = 11.84TPLMEE189 pKa = 4.87PKK191 pKa = 10.49ALVSFSTKK199 pKa = 7.62EE200 pKa = 3.55MRR202 pKa = 11.84PFLGRR207 pKa = 11.84PDD209 pKa = 4.96LLAQEE214 pKa = 4.38LKK216 pKa = 10.25GWLRR220 pKa = 11.84KK221 pKa = 8.45KK222 pKa = 7.47TTVLLLTHH230 pKa = 6.74TEE232 pKa = 4.22TNLKK236 pKa = 9.46QLEE239 pKa = 4.23RR240 pKa = 11.84EE241 pKa = 4.12LRR243 pKa = 11.84DD244 pKa = 3.38MEE246 pKa = 4.34ISFLIADD253 pKa = 3.77QWQEE257 pKa = 3.84CLKK260 pKa = 10.6EE261 pKa = 3.86GRR263 pKa = 11.84LQVGISSLSRR273 pKa = 11.84GFEE276 pKa = 3.71IPGILAVVSAMEE288 pKa = 3.99MYY290 pKa = 10.39GGKK293 pKa = 9.56KK294 pKa = 8.14VRR296 pKa = 11.84RR297 pKa = 11.84AQRR300 pKa = 11.84RR301 pKa = 11.84RR302 pKa = 11.84ATAADD307 pKa = 3.82HH308 pKa = 6.73LPEE311 pKa = 5.64LSLEE315 pKa = 4.43TLWCMSITGSGAMWASVRR333 pKa = 11.84RR334 pKa = 11.84RR335 pKa = 11.84QMISRR340 pKa = 11.84GITWRR345 pKa = 11.84SFMPGRR351 pKa = 11.84QGYY354 pKa = 7.79LCRR357 pKa = 11.84WIRR360 pKa = 11.84WTWFRR365 pKa = 11.84STQVRR370 pKa = 11.84KK371 pKa = 9.6GLHH374 pKa = 5.23RR375 pKa = 11.84VFPGWEE381 pKa = 3.9AVTGRR386 pKa = 11.84ASSSVSKK393 pKa = 10.67SAFRR397 pKa = 11.84SWLKK401 pKa = 10.66ACSLFMRR408 pKa = 11.84SAAMLQVMPFPRR420 pKa = 11.84ILSGSGSLRR429 pKa = 11.84MAFPMRR435 pKa = 11.84RR436 pKa = 11.84HH437 pKa = 7.19RR438 pKa = 11.84IRR440 pKa = 11.84KK441 pKa = 9.13RR442 pKa = 11.84PSEE445 pKa = 4.01MM446 pKa = 3.68

Molecular weight: 51.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3167

0

3167

794821

26

1487

251.0

27.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.849 ± 0.046

1.485 ± 0.023

5.224 ± 0.04

7.02 ± 0.051

4.003 ± 0.03

7.698 ± 0.052

1.85 ± 0.019

7.066 ± 0.045

5.897 ± 0.045

10.363 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.588 ± 0.022

3.611 ± 0.034

4.201 ± 0.031

3.517 ± 0.032

5.372 ± 0.042

5.544 ± 0.034

4.915 ± 0.036

7.411 ± 0.04

1.043 ± 0.018

3.343 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski