Bat coronavirus 1B

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Minunacovirus; Miniopterus bat coronavirus 1

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1PHI9|B1PHI9_9ALPC ORF3 protein OS=Bat coronavirus 1B OX=393768 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.63FFILCIALCLSSVRR15 pKa = 11.84ANIGCEE21 pKa = 4.22SNGNLDD27 pKa = 3.53MQRR30 pKa = 11.84LKK32 pKa = 11.08LGLPSNVTNAYY43 pKa = 9.72VSGYY47 pKa = 10.55LPTPNAWNCTQNPVGIANITYY68 pKa = 8.64NAKK71 pKa = 10.34AVFISYY77 pKa = 10.32YY78 pKa = 9.85KK79 pKa = 10.3FGRR82 pKa = 11.84EE83 pKa = 3.74VTVGVGNSKK92 pKa = 11.14SNDD95 pKa = 3.45SIGIYY100 pKa = 10.17FNHH103 pKa = 6.82HH104 pKa = 6.65NGNKK108 pKa = 8.33STILRR113 pKa = 11.84VCRR116 pKa = 11.84WRR118 pKa = 11.84KK119 pKa = 9.87YY120 pKa = 10.2YY121 pKa = 9.76MPSYY125 pKa = 10.66VPTITTGGEE134 pKa = 3.69DD135 pKa = 4.22CIINVNLKK143 pKa = 10.76LSFLDD148 pKa = 3.6QANHH152 pKa = 6.18SDD154 pKa = 3.1IFGLSWSGDD163 pKa = 3.29RR164 pKa = 11.84LVVYY168 pKa = 10.46SLDD171 pKa = 3.5GVTSYY176 pKa = 10.61HH177 pKa = 6.24IPGIGEE183 pKa = 4.28LDD185 pKa = 3.48VVSFRR190 pKa = 11.84CIFKK194 pKa = 8.22EE195 pKa = 4.41TCAHH199 pKa = 5.53QVITKK204 pKa = 9.03PITVIVNTTEE214 pKa = 3.68EE215 pKa = 4.37GLISGYY221 pKa = 7.95SVCNDD226 pKa = 2.95CAGFPKK232 pKa = 10.67YY233 pKa = 10.65VFAVNEE239 pKa = 4.49GGTIPPNFRR248 pKa = 11.84FDD250 pKa = 2.75NWFYY254 pKa = 10.38LTNSSTPISGMFTSVQPFNLSCVWPIPVLTSNSLPVYY291 pKa = 10.64FNMSLNEE298 pKa = 3.84NATCNGYY305 pKa = 11.16SNFDD309 pKa = 3.62VGVVDD314 pKa = 5.65AMRR317 pKa = 11.84FSLNFTDD324 pKa = 3.87RR325 pKa = 11.84QIIRR329 pKa = 11.84KK330 pKa = 8.92GVISVFTSNNVYY342 pKa = 10.56NFSCTNSSVLGGEE355 pKa = 3.96AVIPFGDD362 pKa = 3.5VSQTHH367 pKa = 5.48YY368 pKa = 11.17CYY370 pKa = 9.34ITYY373 pKa = 7.6FTNTTTGANASQFVGILPPQVKK395 pKa = 9.71EE396 pKa = 3.69FVIMRR401 pKa = 11.84NGDD404 pKa = 3.34FHH406 pKa = 8.25LNGYY410 pKa = 10.04RR411 pKa = 11.84IFSVEE416 pKa = 4.03TVEE419 pKa = 5.39SVIFNITTNDD429 pKa = 3.05GRR431 pKa = 11.84DD432 pKa = 3.31FWTVAFANNAEE443 pKa = 4.29VLTEE447 pKa = 4.26LNATSIQNLLYY458 pKa = 10.82CNNPVNSIKK467 pKa = 10.55CQQLRR472 pKa = 11.84FNLDD476 pKa = 2.92NGFYY480 pKa = 10.78SHH482 pKa = 6.97TFDD485 pKa = 5.15SADD488 pKa = 3.42NLPRR492 pKa = 11.84TIVRR496 pKa = 11.84LPKK499 pKa = 10.3YY500 pKa = 8.32VTHH503 pKa = 6.55SFINVSVGVSFDD515 pKa = 4.08DD516 pKa = 4.47KK517 pKa = 11.62SLAHH521 pKa = 5.71YY522 pKa = 10.41LIDD525 pKa = 3.98FSEE528 pKa = 4.75HH529 pKa = 6.07EE530 pKa = 4.23EE531 pKa = 4.94FIGKK535 pKa = 9.37DD536 pKa = 2.97NRR538 pKa = 11.84TIVCVEE544 pKa = 3.93TTTFTTKK551 pKa = 10.72LKK553 pKa = 10.72VLAFSSFSTAIDD565 pKa = 3.46IQAGSCPFSYY575 pKa = 11.4NNLNNYY581 pKa = 9.99LGFGSLCFSNKK592 pKa = 9.46PNGGCMMSVVAKK604 pKa = 10.4GYY606 pKa = 9.88FGEE609 pKa = 4.28FQKK612 pKa = 11.28VGVLYY617 pKa = 10.69VSYY620 pKa = 10.47TKK622 pKa = 10.93GDD624 pKa = 3.48NVLGVPANNLPTIGVSDD641 pKa = 3.75MSDD644 pKa = 2.75VKK646 pKa = 11.41LNVCTTFTIYY656 pKa = 10.65GHH658 pKa = 5.54TGRR661 pKa = 11.84GIINKK666 pKa = 9.37SNSSFVSGLFYY677 pKa = 10.85TSVTGNLLGFKK688 pKa = 10.45NSTTGEE694 pKa = 4.0IFSISPCQLTTQVAVVSNNIVGVAAATQEE723 pKa = 4.02VKK725 pKa = 10.97LPFNSHH731 pKa = 4.83VNLGSFYY738 pKa = 10.46YY739 pKa = 10.29HH740 pKa = 5.66YY741 pKa = 9.08TNSSAEE747 pKa = 4.07LCKK750 pKa = 10.29TPSLMYY756 pKa = 10.54GGLGVCEE763 pKa = 4.99DD764 pKa = 3.61GRR766 pKa = 11.84LINISKK772 pKa = 10.65SEE774 pKa = 4.05DD775 pKa = 3.46TFVASAVISGNITIPANFSFVVEE798 pKa = 4.14PEE800 pKa = 4.26YY801 pKa = 10.57IQIMTKK807 pKa = 9.91PVSIDD812 pKa = 3.12CSVYY816 pKa = 9.87VCNGNPRR823 pKa = 11.84CLQLLTQYY831 pKa = 11.67ASVCRR836 pKa = 11.84NVEE839 pKa = 3.87EE840 pKa = 4.19PLQLNARR847 pKa = 11.84LEE849 pKa = 4.16ALEE852 pKa = 4.37LTSMIVVQEE861 pKa = 4.05RR862 pKa = 11.84TLDD865 pKa = 3.54LGVAANFNDD874 pKa = 4.09TFDD877 pKa = 3.89LTLALPRR884 pKa = 11.84QRR886 pKa = 11.84QSRR889 pKa = 11.84SAIEE893 pKa = 4.59DD894 pKa = 3.64LLFTKK899 pKa = 10.58VITSGLGTVDD909 pKa = 3.93EE910 pKa = 5.23DD911 pKa = 4.18YY912 pKa = 10.78KK913 pKa = 11.54ACAEE917 pKa = 4.02RR918 pKa = 11.84MANTIAEE925 pKa = 4.54AGCVQYY931 pKa = 11.52YY932 pKa = 10.74NGIAVLPGVVDD943 pKa = 4.33PSLLAQYY950 pKa = 8.17TAALTGGMVLGGVTAAVAIPFSLAVQSRR978 pKa = 11.84LNYY981 pKa = 10.06LALQTDD987 pKa = 4.08VLQRR991 pKa = 11.84NQKK994 pKa = 9.41ILAQSFNAAMGNITVAFSGVSNAIQQTVSSLTTISRR1030 pKa = 11.84ALNKK1034 pKa = 9.92VEE1036 pKa = 4.26NAVNEE1041 pKa = 4.13QGTALSQLTKK1051 pKa = 10.61QLASNFQAVSSSIEE1065 pKa = 3.82DD1066 pKa = 3.6LYY1068 pKa = 11.79NRR1070 pKa = 11.84LDD1072 pKa = 3.65RR1073 pKa = 11.84LEE1075 pKa = 5.39ADD1077 pKa = 3.51QQVDD1081 pKa = 3.46RR1082 pKa = 11.84LITGRR1087 pKa = 11.84LAALNAFVTQQLLRR1101 pKa = 11.84YY1102 pKa = 8.9SEE1104 pKa = 4.28VRR1106 pKa = 11.84ASRR1109 pKa = 11.84QLAQEE1114 pKa = 4.75KK1115 pKa = 10.45INEE1118 pKa = 4.44CVKK1121 pKa = 10.53SQSSRR1126 pKa = 11.84YY1127 pKa = 8.35GFCGNGTHH1135 pKa = 5.87VFSVANAAPDD1145 pKa = 4.45GIMFLHH1151 pKa = 6.91ANLVPTAFIEE1161 pKa = 4.41VSAFAGVCVDD1171 pKa = 3.46GRR1173 pKa = 11.84ALVLRR1178 pKa = 11.84GRR1180 pKa = 11.84DD1181 pKa = 3.0EE1182 pKa = 4.97VLFQKK1187 pKa = 10.79PNTNKK1192 pKa = 10.36YY1193 pKa = 10.45LITPRR1198 pKa = 11.84LLFEE1202 pKa = 4.25PRR1204 pKa = 11.84VPVSADD1210 pKa = 3.25FVEE1213 pKa = 4.87VSSCNVTFVNLTVNEE1228 pKa = 4.25LPEE1231 pKa = 4.63LLPDD1235 pKa = 4.18YY1236 pKa = 10.98IDD1238 pKa = 3.45VNKK1241 pKa = 9.25TLKK1244 pKa = 10.34EE1245 pKa = 3.88FAATIPNRR1253 pKa = 11.84TEE1255 pKa = 3.59MQLTLNTYY1263 pKa = 9.78NATVLNLTDD1272 pKa = 3.91EE1273 pKa = 4.67VNSLTLQASEE1283 pKa = 4.09LTKK1286 pKa = 10.31IASEE1290 pKa = 4.29LNLTISKK1297 pKa = 10.27INNTLVEE1304 pKa = 4.58LEE1306 pKa = 3.96WLSRR1310 pKa = 11.84VEE1312 pKa = 5.38TYY1314 pKa = 10.44ISWPWYY1320 pKa = 7.7VWLAISVTLIILVGLMLWCCLATGCCGCYY1349 pKa = 9.96NCLLKK1354 pKa = 10.86SCSDD1358 pKa = 4.09CGGNAYY1364 pKa = 10.54NVTKK1368 pKa = 10.45LRR1370 pKa = 11.84KK1371 pKa = 8.49STFSNVGWSLSVAII1385 pKa = 5.06

Molecular weight: 151.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1PHJ2|B1PHJ2_9ALPC Nucleoprotein OS=Bat coronavirus 1B OX=393768 GN=N PE=4 SV=1
MM1 pKa = 7.75ASVQFEE7 pKa = 4.47DD8 pKa = 3.64TNSRR12 pKa = 11.84GRR14 pKa = 11.84SGRR17 pKa = 11.84TPLSYY22 pKa = 10.46YY23 pKa = 10.85APLRR27 pKa = 11.84VADD30 pKa = 5.06DD31 pKa = 3.89KK32 pKa = 11.82LFLKK36 pKa = 10.66SMPGNGVPKK45 pKa = 10.76GMGEE49 pKa = 3.72KK50 pKa = 10.04SEE52 pKa = 4.18QIGYY56 pKa = 6.59WTEE59 pKa = 3.6QRR61 pKa = 11.84RR62 pKa = 11.84WRR64 pKa = 11.84MNKK67 pKa = 8.32GQRR70 pKa = 11.84KK71 pKa = 8.92DD72 pKa = 4.4LPSNWHH78 pKa = 6.54FYY80 pKa = 10.83YY81 pKa = 10.82LGTGPHH87 pKa = 6.91ADD89 pKa = 2.92LKK91 pKa = 10.62FRR93 pKa = 11.84EE94 pKa = 4.21RR95 pKa = 11.84QVGVFWVAKK104 pKa = 9.45QGSKK108 pKa = 9.43TEE110 pKa = 4.03PTKK113 pKa = 11.0LGTRR117 pKa = 11.84NRR119 pKa = 11.84NQQLRR124 pKa = 11.84IPTFDD129 pKa = 3.04FTLPDD134 pKa = 3.1SLEE137 pKa = 4.06IVNSDD142 pKa = 2.95SRR144 pKa = 11.84PASRR148 pKa = 11.84SNSRR152 pKa = 11.84SQSKK156 pKa = 9.97SANQSRR162 pKa = 11.84SNSQVRR168 pKa = 11.84EE169 pKa = 3.77QSGNRR174 pKa = 11.84TPKK177 pKa = 10.36NASQKK182 pKa = 10.69KK183 pKa = 8.16NQQEE187 pKa = 4.11DD188 pKa = 3.58LVAAVRR194 pKa = 11.84EE195 pKa = 4.16ALKK198 pKa = 11.07GLGFQQSRR206 pKa = 11.84SSSGRR211 pKa = 11.84STPSRR216 pKa = 11.84AKK218 pKa = 10.61SPARR222 pKa = 11.84QSKK225 pKa = 9.51KK226 pKa = 10.35QLDD229 pKa = 3.56KK230 pKa = 11.38PEE232 pKa = 4.08WKK234 pKa = 9.82RR235 pKa = 11.84VPNRR239 pKa = 11.84TEE241 pKa = 3.54NATTCFGQRR250 pKa = 11.84SVEE253 pKa = 4.24KK254 pKa = 9.47NCGSASVLAMGVEE267 pKa = 4.61TPNFPRR273 pKa = 11.84IAEE276 pKa = 4.92LIPTQAALFFGSRR289 pKa = 11.84VSTKK293 pKa = 10.17EE294 pKa = 3.45NSDD297 pKa = 3.45TVEE300 pKa = 3.57IKK302 pKa = 10.26YY303 pKa = 9.8HH304 pKa = 5.79YY305 pKa = 10.05KK306 pKa = 9.87MSVPKK311 pKa = 9.99TNPNLPYY318 pKa = 10.51FLQQVNAYY326 pKa = 9.7LDD328 pKa = 4.12PNSDD332 pKa = 3.17QPIPKK337 pKa = 9.0KK338 pKa = 10.51QKK340 pKa = 10.47AKK342 pKa = 10.21TEE344 pKa = 4.05EE345 pKa = 4.23TPQLNPTAPAFTHH358 pKa = 6.19PAVPDD363 pKa = 3.6VEE365 pKa = 5.34LEE367 pKa = 4.17MVDD370 pKa = 3.66EE371 pKa = 4.78VFDD374 pKa = 4.29IDD376 pKa = 4.1PLGDD380 pKa = 3.55SVAA383 pKa = 3.81

Molecular weight: 42.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

13628

74

6988

1946.9

215.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.765 ± 0.211

3.383 ± 0.439

5.496 ± 0.514

4.293 ± 0.134

5.988 ± 0.207

6.472 ± 0.215

1.746 ± 0.141

5.188 ± 0.258

6.149 ± 0.536

8.145 ± 0.384

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.062 ± 0.155

6.23 ± 0.376

3.368 ± 0.546

2.502 ± 0.545

2.95 ± 0.606

7.367 ± 0.382

6.164 ± 0.307

10.412 ± 0.702

1.079 ± 0.117

4.241 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski