Mycobacterium phage Andies

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nclasvirinae; Charlievirus; unclassified Charlievirus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4PG35|A0A2H4PG35_9CAUD Uncharacterized protein OS=Mycobacterium phage Andies OX=2047834 GN=SEA_ANDIES_56 PE=4 SV=1
MM1 pKa = 7.47LPDD4 pKa = 4.77CALCGCPYY12 pKa = 10.63HH13 pKa = 7.24EE14 pKa = 4.9DD15 pKa = 3.27RR16 pKa = 11.84CRR18 pKa = 11.84TVVGAAEE25 pKa = 4.44YY26 pKa = 10.38IFGYY30 pKa = 10.85LPMFCDD36 pKa = 3.66CPGYY40 pKa = 10.42EE41 pKa = 4.28EE42 pKa = 5.72PEE44 pKa = 4.33DD45 pKa = 3.68ATT47 pKa = 4.17

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4PFU4|A0A2H4PFU4_9CAUD Uncharacterized protein OS=Mycobacterium phage Andies OX=2047834 GN=SEA_ANDIES_60 PE=4 SV=1
MM1 pKa = 6.97TRR3 pKa = 11.84CALRR7 pKa = 11.84PMNFSVRR14 pKa = 11.84NLMPQRR20 pKa = 11.84EE21 pKa = 4.13ASGDD25 pKa = 3.85SVATAIRR32 pKa = 11.84IGLARR37 pKa = 11.84TNKK40 pKa = 8.43TQTALARR47 pKa = 11.84HH48 pKa = 6.41LKK50 pKa = 10.13LSQPSVHH57 pKa = 6.19RR58 pKa = 11.84RR59 pKa = 11.84MSGKK63 pKa = 9.68VPWRR67 pKa = 11.84IHH69 pKa = 5.21EE70 pKa = 4.12LAAAAEE76 pKa = 4.27FLGITVPDD84 pKa = 3.97LLDD87 pKa = 3.73EE88 pKa = 4.79EE89 pKa = 4.98KK90 pKa = 10.54ATAA93 pKa = 3.98

Molecular weight: 10.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

14230

41

1046

218.9

23.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.778 ± 0.646

1.223 ± 0.18

6.697 ± 0.207

5.805 ± 0.341

2.916 ± 0.179

8.89 ± 0.499

2.157 ± 0.195

4.469 ± 0.171

2.902 ± 0.185

7.751 ± 0.284

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.087 ± 0.136

3.057 ± 0.216

5.966 ± 0.272

3.725 ± 0.214

6.992 ± 0.406

5.186 ± 0.231

6.796 ± 0.268

7.245 ± 0.269

1.918 ± 0.148

2.439 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski