Comamonadaceae bacterium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; unclassified Comamonadaceae

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4276 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q3LC80|A0A4Q3LC80_9BURK DNA mismatch repair protein MutL OS=Comamonadaceae bacterium OX=1871071 GN=mutL PE=3 SV=1
MM1 pKa = 7.23SAVAEE6 pKa = 4.24NVSTEE11 pKa = 3.94MPAPLLFTDD20 pKa = 4.0SAAAKK25 pKa = 10.02VADD28 pKa = 5.45LIAEE32 pKa = 4.31EE33 pKa = 4.82GNPDD37 pKa = 2.99LKK39 pKa = 11.13LRR41 pKa = 11.84VFVQGGGCSGFQYY54 pKa = 10.9GFTFDD59 pKa = 4.44EE60 pKa = 4.63VANDD64 pKa = 4.32DD65 pKa = 4.07DD66 pKa = 4.79TVMTKK71 pKa = 10.67NGVSLLIDD79 pKa = 3.54AMSYY83 pKa = 10.32QYY85 pKa = 11.5LVGAEE90 pKa = 3.79IDD92 pKa = 3.91YY93 pKa = 11.36KK94 pKa = 11.19EE95 pKa = 4.67DD96 pKa = 3.23LQGAQFVIKK105 pKa = 10.4NPNATTTCGCGSSFSAA121 pKa = 4.79

Molecular weight: 12.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q3LJC8|A0A4Q3LJC8_9BURK ATP-dependent Clp protease proteolytic subunit OS=Comamonadaceae bacterium OX=1871071 GN=EOO30_08605 PE=3 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84GAWRR8 pKa = 11.84ARR10 pKa = 11.84WLRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84CGGWRR20 pKa = 11.84AGRR23 pKa = 11.84AARR26 pKa = 11.84NRR28 pKa = 11.84ALLLPRR34 pKa = 11.84KK35 pKa = 9.33ARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84PVFAARR45 pKa = 11.84PPPPAGVRR53 pKa = 11.84RR54 pKa = 11.84PPRR57 pKa = 11.84RR58 pKa = 11.84SRR60 pKa = 11.84AHH62 pKa = 6.72AAPAAPP68 pKa = 4.18

Molecular weight: 7.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4276

0

4276

1326477

25

4605

310.2

33.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.363 ± 0.066

0.897 ± 0.012

5.003 ± 0.03

5.61 ± 0.039

3.493 ± 0.026

8.608 ± 0.041

2.274 ± 0.019

3.935 ± 0.033

3.081 ± 0.04

10.69 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.46 ± 0.017

2.306 ± 0.021

5.503 ± 0.027

3.966 ± 0.024

7.515 ± 0.046

4.853 ± 0.03

4.852 ± 0.034

7.948 ± 0.033

1.519 ± 0.014

2.122 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski