Planctomycetes bacterium MalM25

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; unclassified Planctomycetes

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3738 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A517TGN6|A0A517TGN6_9BACT Uncharacterized protein OS=Planctomycetes bacterium MalM25 OX=2527998 GN=MalM25_04320 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.12SLCIALATCCSLALFQGTASALDD25 pKa = 3.49IYY27 pKa = 11.34VDD29 pKa = 3.91NFDD32 pKa = 4.96SYY34 pKa = 12.02DD35 pKa = 3.45AGSDD39 pKa = 2.78IGGPNWQPKK48 pKa = 7.43WAADD52 pKa = 3.56STQQALFKK60 pKa = 10.52AAAGGEE66 pKa = 4.65GYY68 pKa = 10.88GVIDD72 pKa = 3.28TTVAEE77 pKa = 3.83RR78 pKa = 11.84QYY80 pKa = 11.19RR81 pKa = 11.84VNGKK85 pKa = 9.87SGFSLLAGDD94 pKa = 4.46TVNVAADD101 pKa = 3.68FRR103 pKa = 11.84YY104 pKa = 10.0HH105 pKa = 7.72LEE107 pKa = 4.26AGGDD111 pKa = 3.53APAVFNSNVIGLQLSDD127 pKa = 4.95SEE129 pKa = 4.43NWWAGSRR136 pKa = 11.84KK137 pKa = 9.57SVSLANRR144 pKa = 11.84GPAMGNRR151 pKa = 11.84LPVAPWVQNWIPHH164 pKa = 5.62TSLGIADD171 pKa = 4.27PALGGISEE179 pKa = 4.86WININLEE186 pKa = 4.19LMVSDD191 pKa = 4.22GTALTTAGDD200 pKa = 4.34MIPAGNIYY208 pKa = 10.48GSATFTPEE216 pKa = 3.66SLAGEE221 pKa = 4.17PVEE224 pKa = 4.33NLPVTDD230 pKa = 4.64VLDD233 pKa = 4.18LGYY236 pKa = 8.52TAGTMLYY243 pKa = 10.71AGFTTDD249 pKa = 2.72WTSVAEE255 pKa = 4.31GEE257 pKa = 4.32PTTVSSYY264 pKa = 11.42ASVSEE269 pKa = 3.98VNIDD273 pKa = 3.6NFSLVSDD280 pKa = 4.47AEE282 pKa = 4.33APAGDD287 pKa = 4.21YY288 pKa = 10.93NQDD291 pKa = 3.43GYY293 pKa = 11.95VDD295 pKa = 3.63AADD298 pKa = 3.54YY299 pKa = 8.12TIWRR303 pKa = 11.84DD304 pKa = 3.29SNGQVGGGLAADD316 pKa = 4.02GTGDD320 pKa = 4.31DD321 pKa = 4.68LLGVPDD327 pKa = 4.66GDD329 pKa = 3.56VDD331 pKa = 4.67SFDD334 pKa = 4.17YY335 pKa = 11.2DD336 pKa = 3.2FWDD339 pKa = 3.53ANYY342 pKa = 10.58GSSAFSAPPVATAVPEE358 pKa = 4.55PGTLALVGFAALVAFRR374 pKa = 11.84RR375 pKa = 11.84RR376 pKa = 3.36

Molecular weight: 39.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A517TGM5|A0A517TGM5_9BACT Thioesterase (YiiD_Cterm) OS=Planctomycetes bacterium MalM25 OX=2527998 GN=MalM25_04290 PE=4 SV=1
MM1 pKa = 7.65LGFPLATALIVAGPAWASLGVALAAVAGFLAHH33 pKa = 7.01EE34 pKa = 4.49PLLVIRR40 pKa = 11.84GRR42 pKa = 11.84RR43 pKa = 11.84GLRR46 pKa = 11.84AQRR49 pKa = 11.84EE50 pKa = 4.22APAAKK55 pKa = 9.28QWLWAYY61 pKa = 10.79ALIATTGSSLAFGLGSSAVRR81 pKa = 11.84WSLLCCGAIATAGFALAWAGKK102 pKa = 7.58HH103 pKa = 5.73HH104 pKa = 6.61SRR106 pKa = 11.84AGQAFGAVGLSAPCVPILLSEE127 pKa = 4.37SEE129 pKa = 4.17ASIATAAEE137 pKa = 5.72FWATWLIGLAAAMIAVNSVLAPAKK161 pKa = 10.2RR162 pKa = 11.84LARR165 pKa = 11.84PTATGLLFLLGVATAILQAIGCHH188 pKa = 6.1LPMAVIPVFGLAMTLLLYY206 pKa = 9.75PPRR209 pKa = 11.84PKK211 pKa = 10.13HH212 pKa = 5.65LKK214 pKa = 9.35RR215 pKa = 11.84VGWTLAGGVFATSIWLLTLCC235 pKa = 5.25

Molecular weight: 24.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3738

0

3738

1380535

29

6256

369.3

40.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.367 ± 0.05

0.991 ± 0.017

6.361 ± 0.039

6.758 ± 0.044

3.355 ± 0.025

8.746 ± 0.041

1.997 ± 0.02

3.994 ± 0.024

2.947 ± 0.034

10.068 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.881 ± 0.016

2.755 ± 0.03

5.677 ± 0.03

3.307 ± 0.022

7.01 ± 0.046

5.798 ± 0.03

5.61 ± 0.032

7.375 ± 0.03

1.558 ± 0.018

2.444 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski