Amino acid dipepetide frequency for Natrialba phage PhiCh1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.417AlaAla: 7.417 ± 0.67
0.382AlaCys: 0.382 ± 0.152
7.199AlaAsp: 7.199 ± 0.7
7.635AlaGlu: 7.635 ± 0.683
2.072AlaPhe: 2.072 ± 0.339
7.526AlaGly: 7.526 ± 0.745
1.254AlaHis: 1.254 ± 0.314
5.236AlaIle: 5.236 ± 0.501
3.0AlaLys: 3.0 ± 0.41
6.108AlaLeu: 6.108 ± 0.831
3.818AlaMet: 3.818 ± 1.194
2.672AlaAsn: 2.672 ± 0.371
2.454AlaPro: 2.454 ± 0.384
2.072AlaGln: 2.072 ± 0.317
4.527AlaArg: 4.527 ± 0.419
6.599AlaSer: 6.599 ± 0.848
5.89AlaThr: 5.89 ± 0.71
6.272AlaVal: 6.272 ± 1.227
1.582AlaTrp: 1.582 ± 0.338
2.072AlaTyr: 2.072 ± 0.27
0.0AlaXaa: 0.0 ± 0.0
Cys
0.654CysAla: 0.654 ± 0.173
0.164CysCys: 0.164 ± 0.127
0.491CysAsp: 0.491 ± 0.185
0.491CysGlu: 0.491 ± 0.159
0.273CysPhe: 0.273 ± 0.148
1.145CysGly: 1.145 ± 0.274
0.764CysHis: 0.764 ± 0.189
0.436CysIle: 0.436 ± 0.173
0.327CysLys: 0.327 ± 0.144
0.382CysLeu: 0.382 ± 0.131
0.055CysMet: 0.055 ± 0.05
0.273CysAsn: 0.273 ± 0.118
0.545CysPro: 0.545 ± 0.199
0.6CysGln: 0.6 ± 0.219
0.873CysArg: 0.873 ± 0.244
0.764CysSer: 0.764 ± 0.278
0.436CysThr: 0.436 ± 0.16
0.873CysVal: 0.873 ± 0.197
0.273CysTrp: 0.273 ± 0.106
0.055CysTyr: 0.055 ± 0.061
0.0CysXaa: 0.0 ± 0.0
Asp
10.635AspAla: 10.635 ± 0.914
0.927AspCys: 0.927 ± 0.248
12.216AspAsp: 12.216 ± 1.105
10.635AspGlu: 10.635 ± 0.86
2.291AspPhe: 2.291 ± 0.316
8.235AspGly: 8.235 ± 0.71
2.291AspHis: 2.291 ± 0.366
4.745AspIle: 4.745 ± 0.549
1.145AspLys: 1.145 ± 0.249
7.853AspLeu: 7.853 ± 0.657
1.145AspMet: 1.145 ± 0.232
1.691AspAsn: 1.691 ± 0.308
4.854AspPro: 4.854 ± 0.439
2.018AspGln: 2.018 ± 0.26
7.035AspArg: 7.035 ± 0.638
5.454AspSer: 5.454 ± 0.863
3.763AspThr: 3.763 ± 0.419
9.817AspVal: 9.817 ± 0.77
1.473AspTrp: 1.473 ± 0.24
2.836AspTyr: 2.836 ± 0.275
0.0AspXaa: 0.0 ± 0.0
Glu
8.562GluAla: 8.562 ± 0.675
1.145GluCys: 1.145 ± 0.308
9.926GluAsp: 9.926 ± 0.734
9.49GluGlu: 9.49 ± 0.901
4.254GluPhe: 4.254 ± 0.441
5.617GluGly: 5.617 ± 0.582
2.836GluHis: 2.836 ± 0.472
5.127GluIle: 5.127 ± 0.6
3.054GluLys: 3.054 ± 0.469
8.835GluLeu: 8.835 ± 0.65
2.618GluMet: 2.618 ± 0.37
2.672GluAsn: 2.672 ± 0.384
4.745GluPro: 4.745 ± 0.545
3.381GluGln: 3.381 ± 0.494
6.545GluArg: 6.545 ± 0.668
5.508GluSer: 5.508 ± 0.614
6.435GluThr: 6.435 ± 0.652
6.654GluVal: 6.654 ± 0.631
1.909GluTrp: 1.909 ± 0.345
2.781GluTyr: 2.781 ± 0.415
0.0GluXaa: 0.0 ± 0.0
Phe
2.345PheAla: 2.345 ± 0.318
0.382PheCys: 0.382 ± 0.128
3.381PheAsp: 3.381 ± 0.407
4.418PheGlu: 4.418 ± 0.575
0.654PhePhe: 0.654 ± 0.196
1.745PheGly: 1.745 ± 0.336
0.709PheHis: 0.709 ± 0.16
0.873PheIle: 0.873 ± 0.232
0.873PheLys: 0.873 ± 0.233
1.309PheLeu: 1.309 ± 0.269
0.218PheMet: 0.218 ± 0.107
0.6PheAsn: 0.6 ± 0.151
1.363PhePro: 1.363 ± 0.231
1.091PheGln: 1.091 ± 0.28
1.963PheArg: 1.963 ± 0.33
1.745PheSer: 1.745 ± 0.284
1.745PheThr: 1.745 ± 0.321
2.182PheVal: 2.182 ± 0.352
0.382PheTrp: 0.382 ± 0.141
0.436PheTyr: 0.436 ± 0.131
0.0PheXaa: 0.0 ± 0.0
Gly
4.254GlyAla: 4.254 ± 0.549
0.927GlyCys: 0.927 ± 0.287
8.562GlyAsp: 8.562 ± 0.696
7.908GlyGlu: 7.908 ± 0.749
2.127GlyPhe: 2.127 ± 0.353
6.817GlyGly: 6.817 ± 0.744
1.582GlyHis: 1.582 ± 0.314
3.272GlyIle: 3.272 ± 0.422
1.963GlyLys: 1.963 ± 0.36
5.672GlyLeu: 5.672 ± 0.497
1.854GlyMet: 1.854 ± 0.279
1.691GlyAsn: 1.691 ± 0.287
2.836GlyPro: 2.836 ± 0.401
1.963GlyGln: 1.963 ± 0.353
4.69GlyArg: 4.69 ± 0.516
4.472GlySer: 4.472 ± 0.535
4.145GlyThr: 4.145 ± 0.426
4.745GlyVal: 4.745 ± 0.531
1.418GlyTrp: 1.418 ± 0.231
1.527GlyTyr: 1.527 ± 0.301
0.0GlyXaa: 0.0 ± 0.0
His
1.309HisAla: 1.309 ± 0.282
0.436HisCys: 0.436 ± 0.155
2.291HisAsp: 2.291 ± 0.342
2.018HisGlu: 2.018 ± 0.34
0.382HisPhe: 0.382 ± 0.138
1.909HisGly: 1.909 ± 0.362
0.982HisHis: 0.982 ± 0.262
0.927HisIle: 0.927 ± 0.282
0.6HisLys: 0.6 ± 0.151
1.963HisLeu: 1.963 ± 0.336
0.327HisMet: 0.327 ± 0.15
0.436HisAsn: 0.436 ± 0.179
1.745HisPro: 1.745 ± 0.303
0.764HisGln: 0.764 ± 0.2
2.4HisArg: 2.4 ± 0.434
1.473HisSer: 1.473 ± 0.22
1.036HisThr: 1.036 ± 0.239
1.527HisVal: 1.527 ± 0.281
0.327HisTrp: 0.327 ± 0.17
0.654HisTyr: 0.654 ± 0.174
0.0HisXaa: 0.0 ± 0.0
Ile
4.418IleAla: 4.418 ± 0.502
0.327IleCys: 0.327 ± 0.136
5.672IleAsp: 5.672 ± 0.661
5.945IleGlu: 5.945 ± 0.574
0.873IlePhe: 0.873 ± 0.197
3.545IleGly: 3.545 ± 0.534
0.654IleHis: 0.654 ± 0.206
1.363IleIle: 1.363 ± 0.316
0.764IleLys: 0.764 ± 0.174
2.563IleLeu: 2.563 ± 0.375
0.709IleMet: 0.709 ± 0.191
1.2IleAsn: 1.2 ± 0.237
2.509IlePro: 2.509 ± 0.296
1.909IleGln: 1.909 ± 0.274
3.054IleArg: 3.054 ± 0.417
3.054IleSer: 3.054 ± 0.364
2.727IleThr: 2.727 ± 0.454
3.218IleVal: 3.218 ± 0.366
0.764IleTrp: 0.764 ± 0.297
0.927IleTyr: 0.927 ± 0.231
0.0IleXaa: 0.0 ± 0.0
Lys
2.236LysAla: 2.236 ± 0.322
0.164LysCys: 0.164 ± 0.09
1.2LysAsp: 1.2 ± 0.216
1.854LysGlu: 1.854 ± 0.338
1.036LysPhe: 1.036 ± 0.245
1.745LysGly: 1.745 ± 0.308
0.982LysHis: 0.982 ± 0.216
0.982LysIle: 0.982 ± 0.174
1.036LysLys: 1.036 ± 0.258
1.473LysLeu: 1.473 ± 0.262
0.491LysMet: 0.491 ± 0.191
1.145LysAsn: 1.145 ± 0.241
1.473LysPro: 1.473 ± 0.276
1.091LysGln: 1.091 ± 0.233
2.291LysArg: 2.291 ± 0.355
2.018LysSer: 2.018 ± 0.338
2.018LysThr: 2.018 ± 0.354
1.254LysVal: 1.254 ± 0.276
0.382LysTrp: 0.382 ± 0.148
1.091LysTyr: 1.091 ± 0.283
0.0LysXaa: 0.0 ± 0.0
Leu
6.381LeuAla: 6.381 ± 0.626
0.709LeuCys: 0.709 ± 0.191
7.199LeuAsp: 7.199 ± 0.58
9.271LeuGlu: 9.271 ± 0.568
1.909LeuPhe: 1.909 ± 0.373
3.927LeuGly: 3.927 ± 0.464
1.527LeuHis: 1.527 ± 0.317
2.727LeuIle: 2.727 ± 0.422
1.745LeuLys: 1.745 ± 0.324
5.017LeuLeu: 5.017 ± 0.631
0.927LeuMet: 0.927 ± 0.236
2.89LeuAsn: 2.89 ± 0.469
2.945LeuPro: 2.945 ± 0.399
2.182LeuGln: 2.182 ± 0.372
4.145LeuArg: 4.145 ± 0.644
3.818LeuSer: 3.818 ± 0.608
4.418LeuThr: 4.418 ± 0.512
5.345LeuVal: 5.345 ± 0.575
1.145LeuTrp: 1.145 ± 0.213
1.582LeuTyr: 1.582 ± 0.277
0.0LeuXaa: 0.0 ± 0.0
Met
2.072MetAla: 2.072 ± 0.346
0.055MetCys: 0.055 ± 0.054
2.509MetAsp: 2.509 ± 1.047
1.909MetGlu: 1.909 ± 0.318
0.382MetPhe: 0.382 ± 0.124
1.254MetGly: 1.254 ± 0.245
0.436MetHis: 0.436 ± 0.152
0.982MetIle: 0.982 ± 0.266
0.709MetLys: 0.709 ± 0.241
1.036MetLeu: 1.036 ± 0.218
0.6MetMet: 0.6 ± 0.167
0.6MetAsn: 0.6 ± 0.172
1.2MetPro: 1.2 ± 0.22
0.491MetGln: 0.491 ± 0.175
0.873MetArg: 0.873 ± 0.193
2.345MetSer: 2.345 ± 0.306
2.127MetThr: 2.127 ± 0.323
1.036MetVal: 1.036 ± 0.237
0.109MetTrp: 0.109 ± 0.07
0.491MetTyr: 0.491 ± 0.16
0.0MetXaa: 0.0 ± 0.0
Asn
2.182AsnAla: 2.182 ± 0.276
0.436AsnCys: 0.436 ± 0.151
2.072AsnAsp: 2.072 ± 0.373
2.836AsnGlu: 2.836 ± 0.483
0.327AsnPhe: 0.327 ± 0.129
2.727AsnGly: 2.727 ± 0.392
0.382AsnHis: 0.382 ± 0.166
1.418AsnIle: 1.418 ± 0.248
0.436AsnLys: 0.436 ± 0.177
2.4AsnLeu: 2.4 ± 0.314
0.327AsnMet: 0.327 ± 0.134
1.091AsnAsn: 1.091 ± 0.261
2.4AsnPro: 2.4 ± 0.359
1.309AsnGln: 1.309 ± 0.23
2.182AsnArg: 2.182 ± 0.411
1.363AsnSer: 1.363 ± 0.3
1.527AsnThr: 1.527 ± 0.256
2.345AsnVal: 2.345 ± 0.366
0.382AsnTrp: 0.382 ± 0.143
0.6AsnTyr: 0.6 ± 0.178
0.0AsnXaa: 0.0 ± 0.0
Pro
4.036ProAla: 4.036 ± 0.551
0.218ProCys: 0.218 ± 0.121
5.29ProAsp: 5.29 ± 0.664
5.836ProGlu: 5.836 ± 0.642
1.691ProPhe: 1.691 ± 0.304
3.163ProGly: 3.163 ± 0.393
0.764ProHis: 0.764 ± 0.281
1.854ProIle: 1.854 ± 0.276
1.145ProLys: 1.145 ± 0.236
3.436ProLeu: 3.436 ± 0.48
0.764ProMet: 0.764 ± 0.198
1.418ProAsn: 1.418 ± 0.297
2.182ProPro: 2.182 ± 0.333
1.036ProGln: 1.036 ± 0.237
3.0ProArg: 3.0 ± 0.427
3.054ProSer: 3.054 ± 0.425
2.781ProThr: 2.781 ± 0.38
2.509ProVal: 2.509 ± 0.302
0.764ProTrp: 0.764 ± 0.179
1.2ProTyr: 1.2 ± 0.314
0.0ProXaa: 0.0 ± 0.0
Gln
2.454GlnAla: 2.454 ± 0.306
0.164GlnCys: 0.164 ± 0.091
2.182GlnAsp: 2.182 ± 0.354
3.109GlnGlu: 3.109 ± 0.397
0.873GlnPhe: 0.873 ± 0.274
1.582GlnGly: 1.582 ± 0.243
0.927GlnHis: 0.927 ± 0.208
1.363GlnIle: 1.363 ± 0.269
0.545GlnLys: 0.545 ± 0.153
3.0GlnLeu: 3.0 ± 0.373
0.6GlnMet: 0.6 ± 0.164
0.982GlnAsn: 0.982 ± 0.239
1.418GlnPro: 1.418 ± 0.295
0.764GlnGln: 0.764 ± 0.226
2.563GlnArg: 2.563 ± 0.301
1.963GlnSer: 1.963 ± 0.361
2.509GlnThr: 2.509 ± 0.57
1.527GlnVal: 1.527 ± 0.263
0.382GlnTrp: 0.382 ± 0.129
0.764GlnTyr: 0.764 ± 0.236
0.0GlnXaa: 0.0 ± 0.0
Arg
6.381ArgAla: 6.381 ± 0.572
0.927ArgCys: 0.927 ± 0.295
6.872ArgAsp: 6.872 ± 0.61
6.599ArgGlu: 6.599 ± 0.751
2.291ArgPhe: 2.291 ± 0.37
3.709ArgGly: 3.709 ± 0.45
2.182ArgHis: 2.182 ± 0.354
2.781ArgIle: 2.781 ± 0.344
1.691ArgLys: 1.691 ± 0.289
3.763ArgLeu: 3.763 ± 0.408
1.636ArgMet: 1.636 ± 0.274
1.309ArgAsn: 1.309 ± 0.239
2.781ArgPro: 2.781 ± 0.471
1.691ArgGln: 1.691 ± 0.267
6.435ArgArg: 6.435 ± 0.954
5.836ArgSer: 5.836 ± 0.779
4.254ArgThr: 4.254 ± 0.57
3.763ArgVal: 3.763 ± 0.488
1.636ArgTrp: 1.636 ± 0.344
1.582ArgTyr: 1.582 ± 0.255
0.0ArgXaa: 0.0 ± 0.0
Ser
5.563SerAla: 5.563 ± 0.591
0.982SerCys: 0.982 ± 0.267
5.781SerAsp: 5.781 ± 0.918
5.945SerGlu: 5.945 ± 0.628
1.582SerPhe: 1.582 ± 0.274
5.781SerGly: 5.781 ± 0.51
1.363SerHis: 1.363 ± 0.311
3.163SerIle: 3.163 ± 0.422
2.509SerLys: 2.509 ± 0.377
2.89SerLeu: 2.89 ± 0.43
1.745SerMet: 1.745 ± 0.32
2.454SerAsn: 2.454 ± 0.331
2.618SerPro: 2.618 ± 0.399
2.127SerGln: 2.127 ± 0.57
4.908SerArg: 4.908 ± 0.705
5.508SerSer: 5.508 ± 0.739
5.127SerThr: 5.127 ± 0.608
4.745SerVal: 4.745 ± 0.609
1.745SerTrp: 1.745 ± 0.259
1.854SerTyr: 1.854 ± 0.264
0.0SerXaa: 0.0 ± 0.0
Thr
6.163ThrAla: 6.163 ± 1.123
0.327ThrCys: 0.327 ± 0.144
4.854ThrAsp: 4.854 ± 0.506
4.745ThrGlu: 4.745 ± 0.576
1.8ThrPhe: 1.8 ± 0.375
4.308ThrGly: 4.308 ± 0.523
1.309ThrHis: 1.309 ± 0.28
4.09ThrIle: 4.09 ± 0.619
2.072ThrLys: 2.072 ± 0.389
4.308ThrLeu: 4.308 ± 0.527
1.363ThrMet: 1.363 ± 0.303
1.909ThrAsn: 1.909 ± 0.336
2.945ThrPro: 2.945 ± 0.412
1.8ThrGln: 1.8 ± 0.321
2.89ThrArg: 2.89 ± 0.421
5.127ThrSer: 5.127 ± 0.646
4.145ThrThr: 4.145 ± 0.577
5.127ThrVal: 5.127 ± 0.54
0.545ThrTrp: 0.545 ± 0.222
1.473ThrTyr: 1.473 ± 0.283
0.0ThrXaa: 0.0 ± 0.0
Val
6.217ValAla: 6.217 ± 0.821
0.545ValCys: 0.545 ± 0.218
9.053ValAsp: 9.053 ± 0.76
7.035ValGlu: 7.035 ± 0.665
2.182ValPhe: 2.182 ± 0.328
4.745ValGly: 4.745 ± 0.562
1.527ValHis: 1.527 ± 0.281
3.381ValIle: 3.381 ± 0.459
1.582ValLys: 1.582 ± 0.279
4.581ValLeu: 4.581 ± 0.528
1.254ValMet: 1.254 ± 0.281
1.8ValAsn: 1.8 ± 0.371
2.836ValPro: 2.836 ± 0.386
2.182ValGln: 2.182 ± 0.285
4.363ValArg: 4.363 ± 0.459
5.563ValSer: 5.563 ± 1.035
3.872ValThr: 3.872 ± 0.435
4.527ValVal: 4.527 ± 0.501
0.709ValTrp: 0.709 ± 0.207
1.854ValTyr: 1.854 ± 0.285
0.0ValXaa: 0.0 ± 0.0
Trp
0.927TrpAla: 0.927 ± 0.199
0.327TrpCys: 0.327 ± 0.131
1.636TrpAsp: 1.636 ± 0.315
1.527TrpGlu: 1.527 ± 0.253
0.382TrpPhe: 0.382 ± 0.14
0.764TrpGly: 0.764 ± 0.196
0.436TrpHis: 0.436 ± 0.166
0.764TrpIle: 0.764 ± 0.19
0.273TrpLys: 0.273 ± 0.115
1.909TrpLeu: 1.909 ± 0.379
0.436TrpMet: 0.436 ± 0.164
0.927TrpAsn: 0.927 ± 0.2
0.873TrpPro: 0.873 ± 0.22
0.382TrpGln: 0.382 ± 0.131
1.254TrpArg: 1.254 ± 0.263
1.145TrpSer: 1.145 ± 0.221
1.145TrpThr: 1.145 ± 0.275
0.873TrpVal: 0.873 ± 0.222
0.273TrpTrp: 0.273 ± 0.122
0.491TrpTyr: 0.491 ± 0.152
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.582TyrAla: 1.582 ± 0.321
0.327TyrCys: 0.327 ± 0.138
2.672TyrAsp: 2.672 ± 0.386
2.945TyrGlu: 2.945 ± 0.408
1.036TyrPhe: 1.036 ± 0.193
2.182TyrGly: 2.182 ± 0.296
0.6TyrHis: 0.6 ± 0.194
0.709TyrIle: 0.709 ± 0.201
0.491TyrLys: 0.491 ± 0.172
1.309TyrLeu: 1.309 ± 0.234
0.273TyrMet: 0.273 ± 0.121
0.982TyrAsn: 0.982 ± 0.206
1.418TyrPro: 1.418 ± 0.251
0.818TyrGln: 0.818 ± 0.219
2.127TyrArg: 2.127 ± 0.36
1.582TyrSer: 1.582 ± 0.283
1.2TyrThr: 1.2 ± 0.242
1.473TyrVal: 1.473 ± 0.301
0.545TyrTrp: 0.545 ± 0.154
0.491TyrTyr: 0.491 ± 0.168
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 98 proteins (18337 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski