Citrobacter phage CR8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; unclassified Kayfunavirus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6PUN3|W6PUN3_9CAUD Hypothetical phage protein OS=Citrobacter phage CR8 OX=1455076 GN=0.38 PE=4 SV=1
MM1 pKa = 7.41EE2 pKa = 5.51RR3 pKa = 11.84NADD6 pKa = 3.32AYY8 pKa = 10.63YY9 pKa = 11.13KK10 pKa = 10.68LVADD14 pKa = 3.98AVKK17 pKa = 10.69AFNEE21 pKa = 4.54RR22 pKa = 11.84IQYY25 pKa = 9.78DD26 pKa = 3.56QIAEE30 pKa = 3.85GDD32 pKa = 3.85EE33 pKa = 4.16YY34 pKa = 11.31HH35 pKa = 7.47DD36 pKa = 4.49ALHH39 pKa = 6.39EE40 pKa = 4.29VVDD43 pKa = 5.08DD44 pKa = 4.09YY45 pKa = 12.0VPHH48 pKa = 6.55YY49 pKa = 9.53YY50 pKa = 10.35SEE52 pKa = 4.33IFTVMAADD60 pKa = 5.4GIDD63 pKa = 3.84HH64 pKa = 6.56EE65 pKa = 5.23FEE67 pKa = 5.3DD68 pKa = 4.33SGLMPEE74 pKa = 4.43SMDD77 pKa = 3.16VTVILQARR85 pKa = 11.84IYY87 pKa = 8.84EE88 pKa = 4.05ALYY91 pKa = 11.01NDD93 pKa = 3.63VQNDD97 pKa = 3.95CGLVWYY103 pKa = 9.12EE104 pKa = 4.6AEE106 pKa = 4.4EE107 pKa = 4.34EE108 pKa = 3.99DD109 pKa = 5.86DD110 pKa = 3.9EE111 pKa = 4.85

Molecular weight: 12.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6PUN0|W6PUN0_9CAUD Predicted antirestriction protein OS=Citrobacter phage CR8 OX=1455076 GN=0.3 PE=4 SV=1
MM1 pKa = 7.37GTPQPSGLHH10 pKa = 5.53VIPVIRR16 pKa = 11.84RR17 pKa = 11.84TGTPMVIDD25 pKa = 4.92WLLCWWKK32 pKa = 11.21NSMKK36 pKa = 9.78TLKK39 pKa = 9.84ATIGLPQTRR48 pKa = 11.84FDD50 pKa = 4.47LMTRR54 pKa = 11.84TT55 pKa = 3.91

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

13387

34

1298

234.9

26.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.136 ± 0.427

0.979 ± 0.166

6.297 ± 0.205

6.842 ± 0.273

3.511 ± 0.164

7.761 ± 0.27

1.823 ± 0.188

4.811 ± 0.162

6.842 ± 0.343

8.053 ± 0.229

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.175 ± 0.174

4.265 ± 0.275

3.436 ± 0.17

4.056 ± 0.292

5.027 ± 0.164

6.342 ± 0.269

5.617 ± 0.252

7.119 ± 0.272

1.374 ± 0.139

3.533 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski