Tomato leaf curl Cotabato virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; unclassified Begomovirus

Average proteome isoelectric point is 7.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1PRJ1|B1PRJ1_9GEMI C4 protein OS=Tomato leaf curl Cotabato virus OX=507475 GN=C4 PE=3 SV=1
MM1 pKa = 7.62PPPRR5 pKa = 11.84RR6 pKa = 11.84FLINAKK12 pKa = 10.18NYY14 pKa = 9.38FLTYY18 pKa = 8.4PQCSLTKK25 pKa = 10.58EE26 pKa = 4.02EE27 pKa = 5.47ALSQLQTLNTPTNKK41 pKa = 10.07KK42 pKa = 9.46YY43 pKa = 10.83IKK45 pKa = 9.66ICRR48 pKa = 11.84EE49 pKa = 3.74LHH51 pKa = 6.25EE52 pKa = 5.87DD53 pKa = 4.22GSPHH57 pKa = 6.5LHH59 pKa = 6.04VLVQFEE65 pKa = 4.73GKK67 pKa = 9.96YY68 pKa = 9.54KK69 pKa = 10.02CQNNRR74 pKa = 11.84FFDD77 pKa = 3.91LVSPNRR83 pKa = 11.84SAHH86 pKa = 5.06FHH88 pKa = 6.56PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.11VKK101 pKa = 11.24SYY103 pKa = 10.79IDD105 pKa = 3.7KK106 pKa = 11.33DD107 pKa = 3.49GDD109 pKa = 3.67TLEE112 pKa = 4.14WGEE115 pKa = 3.84FQIDD119 pKa = 3.47GRR121 pKa = 11.84SGRR124 pKa = 11.84GGQQSANDD132 pKa = 4.12AYY134 pKa = 10.85AKK136 pKa = 10.51ALNSGSKK143 pKa = 10.2SEE145 pKa = 4.05ALNVIKK151 pKa = 10.37EE152 pKa = 4.04LAPKK156 pKa = 10.6DD157 pKa = 3.7FVLQFHH163 pKa = 6.68NLNSNLDD170 pKa = 4.05RR171 pKa = 11.84IFAPPLEE178 pKa = 4.54VFVSPFLSSSFDD190 pKa = 3.44QVPEE194 pKa = 3.87EE195 pKa = 4.25LEE197 pKa = 4.08VWVSEE202 pKa = 4.19NVRR205 pKa = 11.84DD206 pKa = 3.6AAARR210 pKa = 11.84PWRR213 pKa = 11.84PKK215 pKa = 10.45SIVVEE220 pKa = 4.25GDD222 pKa = 2.93SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.09VYY251 pKa = 10.93SNNVWYY257 pKa = 10.67NVIDD261 pKa = 4.54DD262 pKa = 4.33VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.26YY287 pKa = 10.63GKK289 pKa = 9.49PIQIKK294 pKa = 10.42GGIPTIFLCNPGPTSSYY311 pKa = 11.06KK312 pKa = 10.58EE313 pKa = 3.96YY314 pKa = 10.92LDD316 pKa = 3.7EE317 pKa = 5.77DD318 pKa = 4.13KK319 pKa = 11.56NSALKK324 pKa = 10.54DD325 pKa = 3.32WALKK329 pKa = 9.38NAEE332 pKa = 5.18FITITQPLYY341 pKa = 10.97SSTHH345 pKa = 5.26QGPTQNSQEE354 pKa = 4.3KK355 pKa = 9.05TSSQAEE361 pKa = 4.27SS362 pKa = 3.15

Molecular weight: 41.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1PRI8|B1PRI8_9GEMI Replication enhancer OS=Tomato leaf curl Cotabato virus OX=507475 GN=C3 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.52IVISTPASKK16 pKa = 10.29VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.04SPYY27 pKa = 8.83TSRR30 pKa = 11.84AAAPTVLVTNKK41 pKa = 9.33RR42 pKa = 11.84RR43 pKa = 11.84TWSNRR48 pKa = 11.84PMYY51 pKa = 9.83RR52 pKa = 11.84KK53 pKa = 8.83PRR55 pKa = 11.84MYY57 pKa = 10.82RR58 pKa = 11.84MYY60 pKa = 10.57RR61 pKa = 11.84SPDD64 pKa = 3.29VPKK67 pKa = 10.81GCEE70 pKa = 4.39GPCKK74 pKa = 9.35VQSYY78 pKa = 6.94EE79 pKa = 3.74QRR81 pKa = 11.84HH82 pKa = 6.38DD83 pKa = 3.16ISHH86 pKa = 5.75VGKK89 pKa = 9.75VLCVSDD95 pKa = 3.55VTRR98 pKa = 11.84GSGLTHH104 pKa = 6.46RR105 pKa = 11.84VGKK108 pKa = 9.66RR109 pKa = 11.84FCVKK113 pKa = 9.94SVYY116 pKa = 10.52VLGKK120 pKa = 9.41IWMDD124 pKa = 3.35EE125 pKa = 4.04NIKK128 pKa = 9.32TKK130 pKa = 10.61NHH132 pKa = 5.92TNTVMFYY139 pKa = 10.33LVRR142 pKa = 11.84DD143 pKa = 3.68RR144 pKa = 11.84RR145 pKa = 11.84PFGTAMDD152 pKa = 4.48FGQVFNMYY160 pKa = 10.66DD161 pKa = 3.86NEE163 pKa = 4.36PSTATIKK170 pKa = 11.0NDD172 pKa = 2.84FRR174 pKa = 11.84DD175 pKa = 3.45RR176 pKa = 11.84FQVLRR181 pKa = 11.84KK182 pKa = 8.2FTSTVTGGQYY192 pKa = 10.82ASKK195 pKa = 10.11EE196 pKa = 3.81QALVRR201 pKa = 11.84KK202 pKa = 9.32FMKK205 pKa = 9.79VNNYY209 pKa = 7.84VVYY212 pKa = 10.54NHH214 pKa = 6.06QEE216 pKa = 3.36AAKK219 pKa = 10.14YY220 pKa = 10.5DD221 pKa = 3.75NHH223 pKa = 6.5TEE225 pKa = 4.02NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.86ATLKK246 pKa = 9.47IRR248 pKa = 11.84IYY250 pKa = 10.63FYY252 pKa = 11.33DD253 pKa = 3.44SLQNN257 pKa = 3.51

Molecular weight: 29.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1100

96

362

183.3

21.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.182 ± 0.491

2.091 ± 0.377

5.182 ± 0.591

4.364 ± 0.684

4.364 ± 0.459

4.364 ± 0.499

3.909 ± 0.627

4.818 ± 0.963

6.182 ± 0.79

6.909 ± 0.668

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.638

6.091 ± 0.281

5.636 ± 0.661

5.364 ± 0.795

6.364 ± 1.0

8.636 ± 1.178

6.182 ± 0.847

6.455 ± 0.933

1.545 ± 0.291

4.091 ± 0.682
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski