Lake Sarah-associated circular virus-11

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126G9K5|A0A126G9K5_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-11 OX=1685737 PE=4 SV=1
MM1 pKa = 7.74APRR4 pKa = 11.84GNAAKK9 pKa = 10.27RR10 pKa = 11.84WTFTINNWTLADD22 pKa = 3.76YY23 pKa = 11.19DD24 pKa = 3.97KK25 pKa = 11.47VLEE28 pKa = 4.29FTTKK32 pKa = 10.27FEE34 pKa = 4.48PVFLICGKK42 pKa = 10.02EE43 pKa = 3.84RR44 pKa = 11.84GEE46 pKa = 4.48LLTDD50 pKa = 3.68HH51 pKa = 6.7LQGFVHH57 pKa = 6.83LKK59 pKa = 9.61SKK61 pKa = 10.48QRR63 pKa = 11.84LTFLKK68 pKa = 10.79ANLSEE73 pKa = 4.18RR74 pKa = 11.84AHH76 pKa = 6.15FEE78 pKa = 3.93CAKK81 pKa = 10.84GSDD84 pKa = 3.82EE85 pKa = 5.66DD86 pKa = 4.09NRR88 pKa = 11.84DD89 pKa = 3.49YY90 pKa = 11.14CSKK93 pKa = 10.89EE94 pKa = 3.65DD95 pKa = 3.69TEE97 pKa = 4.37PFIFGTPVKK106 pKa = 10.36SCSEE110 pKa = 3.97KK111 pKa = 10.19GGGRR115 pKa = 11.84ITAQQVIGAAIALATEE131 pKa = 4.58RR132 pKa = 11.84EE133 pKa = 4.14PFLVIKK139 pKa = 10.45KK140 pKa = 9.66NEE142 pKa = 3.91EE143 pKa = 3.5FAVPVLRR150 pKa = 11.84HH151 pKa = 4.94YY152 pKa = 11.58ASFEE156 pKa = 3.8KK157 pKa = 10.61LAASVRR163 pKa = 11.84RR164 pKa = 11.84LNALEE169 pKa = 3.92QLQSEE174 pKa = 4.66YY175 pKa = 11.21KK176 pKa = 9.68EE177 pKa = 3.95VRR179 pKa = 11.84LKK181 pKa = 10.56VWQQAIINTIQEE193 pKa = 4.19RR194 pKa = 11.84PHH196 pKa = 6.64PRR198 pKa = 11.84TIHH201 pKa = 6.01WYY203 pKa = 8.2WEE205 pKa = 4.05ATGGTGKK212 pKa = 7.95TWMSKK217 pKa = 10.67YY218 pKa = 10.49LVACHH223 pKa = 5.76SAFRR227 pKa = 11.84AEE229 pKa = 4.16NGKK232 pKa = 10.83SNDD235 pKa = 2.63IKK237 pKa = 10.88YY238 pKa = 10.3AYY240 pKa = 10.01AGEE243 pKa = 4.2KK244 pKa = 10.34VVIFDD249 pKa = 3.58FTRR252 pKa = 11.84SQTEE256 pKa = 4.06HH257 pKa = 6.35INYY260 pKa = 9.83EE261 pKa = 3.91IIEE264 pKa = 4.42SIKK267 pKa = 10.26NGCYY271 pKa = 9.99FNCKK275 pKa = 9.24YY276 pKa = 10.23EE277 pKa = 4.1SGMRR281 pKa = 11.84IFPTPHH287 pKa = 5.68VFCFSNSPPDD297 pKa = 3.92LSKK300 pKa = 10.58MSADD304 pKa = 3.28RR305 pKa = 11.84WHH307 pKa = 6.89ISQIRR312 pKa = 11.84GLLLSWSVPLPIPGTRR328 pKa = 11.84TVDD331 pKa = 3.26EE332 pKa = 4.67SSVCEE337 pKa = 3.98TSSEE341 pKa = 4.2EE342 pKa = 4.13GGEE345 pKa = 3.91EE346 pKa = 4.04SFNLFEE352 pKa = 4.79YY353 pKa = 11.11LDD355 pKa = 3.66ATKK358 pKa = 9.96TT359 pKa = 3.47

Molecular weight: 40.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126G9K5|A0A126G9K5_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-11 OX=1685737 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 10.09YY3 pKa = 10.64ASRR6 pKa = 11.84KK7 pKa = 9.19GRR9 pKa = 11.84GKK11 pKa = 10.37RR12 pKa = 11.84KK13 pKa = 8.37STTSHH18 pKa = 6.9RR19 pKa = 11.84GRR21 pKa = 11.84GGKK24 pKa = 7.78VRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84LFRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84KK34 pKa = 9.07SFLKK38 pKa = 10.38RR39 pKa = 11.84RR40 pKa = 11.84TGNGGFANNTARR52 pKa = 11.84RR53 pKa = 11.84YY54 pKa = 10.37KK55 pKa = 10.51KK56 pKa = 10.58GLTAKK61 pKa = 10.2IVGDD65 pKa = 4.24DD66 pKa = 3.45FTLAIEE72 pKa = 4.35AAGGDD77 pKa = 4.52SNTMLIMYY85 pKa = 9.61DD86 pKa = 3.47GGNLGVSQFAMQQINAYY103 pKa = 7.67STLYY107 pKa = 10.7DD108 pKa = 3.46SYY110 pKa = 10.97KK111 pKa = 9.31VKK113 pKa = 10.55RR114 pKa = 11.84VVAEE118 pKa = 3.6WWLNDD123 pKa = 3.21NDD125 pKa = 4.27EE126 pKa = 4.23LAKK129 pKa = 10.43PDD131 pKa = 3.75TQLVNMWQCYY141 pKa = 9.5DD142 pKa = 3.4HH143 pKa = 7.53DD144 pKa = 4.61AFSKK148 pKa = 10.2VLKK151 pKa = 9.94IDD153 pKa = 4.41SIKK156 pKa = 9.96QQPGVKK162 pKa = 9.1RR163 pKa = 11.84HH164 pKa = 4.73IMMPGRR170 pKa = 11.84VYY172 pKa = 9.69KK173 pKa = 10.54TSFVPKK179 pKa = 10.05FVVNNKK185 pKa = 8.98NNQYY189 pKa = 9.63MEE191 pKa = 4.44GFNTWMDD198 pKa = 3.74LKK200 pKa = 11.18DD201 pKa = 3.34IAKK204 pKa = 9.5TNASGNALQFGWEE217 pKa = 4.27GKK219 pKa = 10.37AEE221 pKa = 3.86EE222 pKa = 4.27AAISVRR228 pKa = 11.84ITYY231 pKa = 9.41YY232 pKa = 10.16FAFGGRR238 pKa = 11.84RR239 pKa = 11.84VNSVYY244 pKa = 10.66QNSVDD249 pKa = 3.85NTVHH253 pKa = 6.88LL254 pKa = 4.49

Molecular weight: 28.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

613

254

359

306.5

34.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.341 ± 0.09

1.631 ± 0.798

4.405 ± 0.714

6.525 ± 2.429

5.383 ± 0.425

7.178 ± 1.21

2.284 ± 0.457

5.057 ± 0.722

7.83 ± 0.789

6.525 ± 0.653

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.121 ± 0.917

5.546 ± 1.246

3.1 ± 0.983

3.426 ± 0.329

6.688 ± 1.018

6.852 ± 0.61

6.199 ± 0.443

5.873 ± 0.782

2.121 ± 0.098

3.915 ± 0.522
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski