Streptomyces sp. CB01635

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8519 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2M9JTU8|A0A2M9JTU8_9ACTN XRE family transcriptional regulator OS=Streptomyces sp. CB01635 OX=2020326 GN=CG723_18175 PE=4 SV=1
MM1 pKa = 7.64HH2 pKa = 7.25SRR4 pKa = 11.84PISRR8 pKa = 11.84RR9 pKa = 11.84TLRR12 pKa = 11.84AASAVMLVVGAGLTGPVLLAGTAGAATSPATAAIDD47 pKa = 3.78GRR49 pKa = 11.84AVAYY53 pKa = 7.55TAAPGQTNDD62 pKa = 3.34VTVTASKK69 pKa = 10.82DD70 pKa = 3.59GADD73 pKa = 3.12ITYY76 pKa = 10.71VIDD79 pKa = 4.03DD80 pKa = 4.17VVTIGAGDD88 pKa = 3.85GCSYY92 pKa = 10.71PSSTDD97 pKa = 3.05QTQITCTITTVDD109 pKa = 3.58SQDD112 pKa = 3.57PYY114 pKa = 12.01ASLEE118 pKa = 4.04LSLGDD123 pKa = 3.79GNDD126 pKa = 2.81VLTYY130 pKa = 10.97DD131 pKa = 3.54NQTDD135 pKa = 3.44EE136 pKa = 4.79TYY138 pKa = 11.13NFASIDD144 pKa = 3.61MGDD147 pKa = 3.95GKK149 pKa = 9.89DD150 pKa = 3.43TLTEE154 pKa = 3.97TGGVQGNGVTGGAGDD169 pKa = 4.74DD170 pKa = 3.88NLSVGPVTVVLGGDD184 pKa = 3.6GNDD187 pKa = 4.27TIDD190 pKa = 3.89AADD193 pKa = 3.64GTVAQGGIGNDD204 pKa = 4.0TIHH207 pKa = 7.06SRR209 pKa = 11.84GEE211 pKa = 3.78DD212 pKa = 3.41SAVDD216 pKa = 3.73GGAGRR221 pKa = 11.84DD222 pKa = 3.91LIYY225 pKa = 10.96GGADD229 pKa = 3.19RR230 pKa = 11.84QSLSGGDD237 pKa = 3.87DD238 pKa = 3.38NDD240 pKa = 4.43TIRR243 pKa = 11.84GGDD246 pKa = 3.54GSDD249 pKa = 3.36YY250 pKa = 10.97LYY252 pKa = 10.98GGKK255 pKa = 10.32GDD257 pKa = 3.7DD258 pKa = 3.61VLYY261 pKa = 11.28GEE263 pKa = 5.58AGDD266 pKa = 3.75DD267 pKa = 3.72TIYY270 pKa = 11.31GNSGNDD276 pKa = 3.29KK277 pKa = 10.55LYY279 pKa = 11.24GGAGTDD285 pKa = 3.71TLSGGPGTNVVHH297 pKa = 6.97QDD299 pKa = 2.85

Molecular weight: 29.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2M9JF95|A0A2M9JF95_9ACTN FAD-linked oxidoreductase OS=Streptomyces sp. CB01635 OX=2020326 GN=CG723_45030 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.72HH17 pKa = 5.74RR18 pKa = 11.84KK19 pKa = 7.77LLKK22 pKa = 8.07RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8519

0

8519

2820413

27

7351

331.1

35.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.359 ± 0.043

0.785 ± 0.007

6.072 ± 0.022

5.533 ± 0.025

2.845 ± 0.015

9.491 ± 0.023

2.332 ± 0.015

3.326 ± 0.018

2.374 ± 0.024

10.228 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.797 ± 0.011

1.842 ± 0.013

5.955 ± 0.024

2.843 ± 0.017

7.72 ± 0.028

5.241 ± 0.022

6.162 ± 0.023

8.43 ± 0.024

1.527 ± 0.011

2.137 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski