Escherichia phage PBECO4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Asteriusvirus; Escherichia virus PBECO4

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 551 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7THW9|L7THW9_9CAUD Uncharacterized protein OS=Escherichia phage PBECO4 OX=1273738 PE=4 SV=1
MM1 pKa = 7.7MYY3 pKa = 9.39TPDD6 pKa = 3.66MIYY9 pKa = 10.79VEE11 pKa = 5.78LEE13 pKa = 4.21DD14 pKa = 6.47DD15 pKa = 3.98DD16 pKa = 6.28DD17 pKa = 4.18GWNLIYY23 pKa = 10.64SRR25 pKa = 11.84SSGWYY30 pKa = 7.52MAHH33 pKa = 6.76LNDD36 pKa = 5.91SIVTDD41 pKa = 3.51YY42 pKa = 11.1FVDD45 pKa = 5.01FNTLSDD51 pKa = 3.9EE52 pKa = 4.66EE53 pKa = 5.47LFQQQLVIDD62 pKa = 3.81TGGYY66 pKa = 10.27DD67 pKa = 3.48VLLLKK72 pKa = 10.41KK73 pKa = 9.57IQKK76 pKa = 10.18AVIDD80 pKa = 4.45FKK82 pKa = 11.57PMIWQRR88 pKa = 11.84KK89 pKa = 7.9YY90 pKa = 10.67YY91 pKa = 9.91DD92 pKa = 3.96LEE94 pKa = 4.12VPYY97 pKa = 10.5QQQ99 pKa = 4.65

Molecular weight: 11.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7TKJ3|L7TKJ3_9CAUD Regulator of chromosome condensation RCC1 OS=Escherichia phage PBECO4 OX=1273738 PE=4 SV=1
MM1 pKa = 7.34NIKK4 pKa = 10.32YY5 pKa = 9.47FATRR9 pKa = 11.84EE10 pKa = 3.92QARR13 pKa = 11.84IAARR17 pKa = 11.84QDD19 pKa = 2.83GATFKK24 pKa = 11.44DD25 pKa = 3.22MGVQSEE31 pKa = 4.36RR32 pKa = 11.84GKK34 pKa = 10.21RR35 pKa = 11.84WAIVKK40 pKa = 10.29RR41 pKa = 11.84STARR45 pKa = 11.84SLQYY49 pKa = 10.04WLAALKK55 pKa = 11.0SPDD58 pKa = 3.45SVSRR62 pKa = 11.84RR63 pKa = 11.84VVEE66 pKa = 4.02IKK68 pKa = 10.53LDD70 pKa = 3.59RR71 pKa = 11.84KK72 pKa = 9.54GNRR75 pKa = 11.84VTVYY79 pKa = 8.29RR80 pKa = 11.84TKK82 pKa = 10.71KK83 pKa = 9.81IAVLEE88 pKa = 4.18

Molecular weight: 10.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

551

0

551

101510

37

3333

184.2

21.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.915 ± 0.154

1.314 ± 0.055

6.645 ± 0.122

6.857 ± 0.147

4.536 ± 0.083

5.56 ± 0.137

1.845 ± 0.066

7.48 ± 0.118

7.002 ± 0.152

7.787 ± 0.098

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.873 ± 0.066

6.318 ± 0.114

3.193 ± 0.09

3.531 ± 0.081

3.673 ± 0.067

7.108 ± 0.129

6.364 ± 0.122

6.644 ± 0.127

1.084 ± 0.05

5.273 ± 0.121
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski