Cronobacter phage vB_CsaP_Ss1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A096XUS6|A0A096XUS6_9CAUD Uncharacterized protein OS=Cronobacter phage vB_CsaP_Ss1 OX=1498011 GN=SS1_01 PE=4 SV=1
MM1 pKa = 6.9YY2 pKa = 9.25TVQDD6 pKa = 3.73CEE8 pKa = 4.08KK9 pKa = 10.02VYY11 pKa = 10.92GVVDD15 pKa = 3.77SLEE18 pKa = 4.04EE19 pKa = 3.92ALALAEE25 pKa = 4.71KK26 pKa = 10.49YY27 pKa = 10.11NAEE30 pKa = 3.8IFDD33 pKa = 4.32ANGEE37 pKa = 4.15LVEE40 pKa = 4.15IAPRR44 pKa = 3.66

Molecular weight: 4.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A096XUW8|A0A096XUW8_9CAUD Uncharacterized protein OS=Cronobacter phage vB_CsaP_Ss1 OX=1498011 GN=SS1_48 PE=4 SV=1
MM1 pKa = 7.61TYY3 pKa = 9.65ATVDD7 pKa = 3.3RR8 pKa = 11.84RR9 pKa = 11.84NLDD12 pKa = 3.26SAKK15 pKa = 10.43PADD18 pKa = 3.66SDD20 pKa = 3.47KK21 pKa = 11.67DD22 pKa = 3.56LFITVFTDD30 pKa = 5.26GSWCPDD36 pKa = 2.99TKK38 pKa = 11.05AYY40 pKa = 10.26GYY42 pKa = 10.6AVWIRR47 pKa = 11.84DD48 pKa = 3.67GQKK51 pKa = 9.44PIHH54 pKa = 6.13MFGAGGFGGRR64 pKa = 11.84DD65 pKa = 3.29SYY67 pKa = 11.44HH68 pKa = 7.22VEE70 pKa = 3.99TLGLKK75 pKa = 10.32AAVKK79 pKa = 10.4YY80 pKa = 10.72ILDD83 pKa = 3.76NCSVKK88 pKa = 10.42EE89 pKa = 3.9RR90 pKa = 11.84VLVIQCDD97 pKa = 3.63NIGALNALDD106 pKa = 3.27IKK108 pKa = 10.07QFKK111 pKa = 10.24KK112 pKa = 10.75LGAKK116 pKa = 9.1FVKK119 pKa = 9.91RR120 pKa = 11.84KK121 pKa = 9.05HH122 pKa = 5.82VKK124 pKa = 9.66AHH126 pKa = 6.27TSHH129 pKa = 5.9KK130 pKa = 8.79TNRR133 pKa = 11.84SKK135 pKa = 11.49VNEE138 pKa = 4.18LVDD141 pKa = 3.89NLAGQYY147 pKa = 8.3MRR149 pKa = 11.84EE150 pKa = 3.82YY151 pKa = 9.29RR152 pKa = 11.84AKK154 pKa = 10.65VRR156 pKa = 11.84GNAA159 pKa = 3.35

Molecular weight: 17.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

13418

41

931

235.4

26.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.563 ± 0.36

1.073 ± 0.132

6.499 ± 0.183

6.216 ± 0.408

3.644 ± 0.178

7.482 ± 0.314

2.258 ± 0.191

6.007 ± 0.276

6.603 ± 0.302

7.572 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.309 ± 0.193

5.016 ± 0.251

3.987 ± 0.241

4.3 ± 0.378

4.509 ± 0.22

5.634 ± 0.293

5.992 ± 0.315

6.007 ± 0.202

1.498 ± 0.135

3.831 ± 0.294
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski