Synechococcus phage S-CAM22

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 214 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8KQP0|A0A1D8KQP0_9CAUD Uncharacterized protein OS=Synechococcus phage S-CAM22 OX=1883365 GN=C350210_182 PE=4 SV=1
MM1 pKa = 7.82AKK3 pKa = 9.86QSLNIGTTANDD14 pKa = 3.37NTGDD18 pKa = 3.91TLRR21 pKa = 11.84SGGDD25 pKa = 3.43KK26 pKa = 10.97INDD29 pKa = 3.69NFDD32 pKa = 3.65EE33 pKa = 5.52LYY35 pKa = 9.94TALGNNASLSISLANPATGQVLKK58 pKa = 11.0YY59 pKa = 9.84NGSSFVAANFNALTSALDD77 pKa = 3.38VSGNSIISSSNGNITLAPNGTGDD100 pKa = 3.69VVITAGSQTTTFDD113 pKa = 3.24GATGGVGFGSTISYY127 pKa = 9.58KK128 pKa = 10.64NEE130 pKa = 3.28YY131 pKa = 8.21TALGNAPAAATFPGYY146 pKa = 10.34FYY148 pKa = 10.64TVDD151 pKa = 3.88GDD153 pKa = 4.01DD154 pKa = 3.81NPYY157 pKa = 11.03VNINITGGGVGDD169 pKa = 3.95TRR171 pKa = 11.84AKK173 pKa = 10.89LLTEE177 pKa = 4.07YY178 pKa = 11.15SSIDD182 pKa = 3.68DD183 pKa = 4.58LSDD186 pKa = 3.25VDD188 pKa = 3.99ITTSAPTSNQVLKK201 pKa = 10.73WNGTKK206 pKa = 10.14FVPADD211 pKa = 3.6DD212 pKa = 4.19AAGAGQQNIFASVAGDD228 pKa = 3.48TGTTTANSVTDD239 pKa = 3.55TLTIAGGTDD248 pKa = 2.59IVTSVSGDD256 pKa = 3.44TVTVAFNGTLTTTFAALTDD275 pKa = 3.68TDD277 pKa = 4.35VPSITQGDD285 pKa = 3.94SLYY288 pKa = 10.39WNGSDD293 pKa = 2.88WVVTRR298 pKa = 11.84SPMTWWEE305 pKa = 3.98VGANGANHH313 pKa = 5.69FTINGPGFSSPTDD326 pKa = 3.53DD327 pKa = 3.18PTLYY331 pKa = 10.43VIRR334 pKa = 11.84GMTYY338 pKa = 11.01AFDD341 pKa = 3.55NTANGTNHH349 pKa = 7.55PFRR352 pKa = 11.84IQSTAGLSGTPYY364 pKa = 8.41TTGQSGSGTSVLYY377 pKa = 7.98WTVPMDD383 pKa = 4.37APNTLYY389 pKa = 10.3YY390 pKa = 10.43QCTIHH395 pKa = 8.33ALMNGTINVLII406 pKa = 4.6

Molecular weight: 41.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8KRT3|A0A1D8KRT3_9CAUD YadA domain-containing structural protein OS=Synechococcus phage S-CAM22 OX=1883365 GN=T191209_086 PE=4 SV=1
MM1 pKa = 7.58AKK3 pKa = 9.1MKK5 pKa = 10.65KK6 pKa = 9.97SLTGGNMIEE15 pKa = 4.23TTPKK19 pKa = 9.31KK20 pKa = 8.67TRR22 pKa = 11.84QGNGQHH28 pKa = 5.71TKK30 pKa = 10.18YY31 pKa = 10.97ASTSRR36 pKa = 11.84NSAKK40 pKa = 9.92KK41 pKa = 9.68RR42 pKa = 11.84YY43 pKa = 9.01RR44 pKa = 11.84GQGRR48 pKa = 3.34

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

214

0

214

55241

26

6273

258.1

28.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.152 ± 0.262

0.86 ± 0.096

6.812 ± 0.127

6.202 ± 0.287

4.281 ± 0.105

7.961 ± 0.361

1.455 ± 0.116

6.158 ± 0.18

5.845 ± 0.333

7.036 ± 0.166

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.113 ± 0.201

5.74 ± 0.203

3.856 ± 0.147

3.662 ± 0.104

3.919 ± 0.179

7.201 ± 0.294

7.713 ± 0.463

6.816 ± 0.16

1.11 ± 0.081

4.106 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski