Spinach latent virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Ilarvirus

Average proteome isoelectric point is 7.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O57162|O57162_9BROM RNA-directed RNA polymerase 2a OS=Spinach latent virus OX=42680 PE=4 SV=1
MM1 pKa = 7.46DD2 pKa = 5.89TIVQMKK8 pKa = 7.34VLCYY12 pKa = 10.6EE13 pKa = 4.13SMILLMIPSDD23 pKa = 3.97VNAPRR28 pKa = 11.84PEE30 pKa = 3.95TAIGRR35 pKa = 11.84LISDD39 pKa = 4.03AAVSGVLRR47 pKa = 11.84QCDD50 pKa = 3.79FTAAEE55 pKa = 4.35ASQCFVPIITGRR67 pKa = 11.84SEE69 pKa = 4.38CIEE72 pKa = 3.91TRR74 pKa = 11.84FSGRR78 pKa = 11.84EE79 pKa = 3.36IQFRR83 pKa = 11.84NFSYY87 pKa = 10.71SSQSFAAAHH96 pKa = 5.94RR97 pKa = 11.84VCEE100 pKa = 3.53ADD102 pKa = 3.43YY103 pKa = 10.5IYY105 pKa = 11.24SRR107 pKa = 11.84FQTRR111 pKa = 11.84NTTIIDD117 pKa = 3.13IGGNFCTHH125 pKa = 5.67SKK127 pKa = 9.27MGRR130 pKa = 11.84DD131 pKa = 3.84NVHH134 pKa = 5.96SCCPILDD141 pKa = 3.72VRR143 pKa = 11.84DD144 pKa = 3.5GARR147 pKa = 11.84YY148 pKa = 7.17TDD150 pKa = 3.1RR151 pKa = 11.84FMSVAGALEE160 pKa = 4.11TQPDD164 pKa = 3.5RR165 pKa = 11.84EE166 pKa = 4.29LRR168 pKa = 11.84LNYY171 pKa = 9.89CSNKK175 pKa = 9.76FEE177 pKa = 5.1DD178 pKa = 5.03CDD180 pKa = 4.59VSAPWAMAIHH190 pKa = 7.06SISDD194 pKa = 3.36IPITSVVKK202 pKa = 9.02HH203 pKa = 5.74CFRR206 pKa = 11.84RR207 pKa = 11.84GVKK210 pKa = 10.07KK211 pKa = 10.81LIASVMMDD219 pKa = 3.07PMMLVASQGFMPRR232 pKa = 11.84LNVQWEE238 pKa = 4.16IEE240 pKa = 3.85PDD242 pKa = 3.56EE243 pKa = 4.3EE244 pKa = 4.53NKK246 pKa = 10.11GSRR249 pKa = 11.84RR250 pKa = 11.84ISFHH254 pKa = 6.68FVDD257 pKa = 5.61APGLSYY263 pKa = 9.32THH265 pKa = 6.8NYY267 pKa = 9.12DD268 pKa = 3.86VLMQYY273 pKa = 7.39MTCNQVIISGKK284 pKa = 8.37AAYY287 pKa = 9.46RR288 pKa = 11.84VEE290 pKa = 4.2RR291 pKa = 11.84VADD294 pKa = 3.92LSGVFIVEE302 pKa = 4.08INPGGNCWNEE312 pKa = 3.9RR313 pKa = 11.84LDD315 pKa = 4.25LVPMRR320 pKa = 11.84DD321 pKa = 3.75VSCAWMTSLKK331 pKa = 10.42RR332 pKa = 11.84KK333 pKa = 7.29TLVRR337 pKa = 11.84VAIPQYY343 pKa = 8.21IHH345 pKa = 5.77SWEE348 pKa = 4.18VKK350 pKa = 10.13YY351 pKa = 11.11VIADD355 pKa = 3.34TDD357 pKa = 3.65FVRR360 pKa = 11.84RR361 pKa = 11.84VAEE364 pKa = 3.96VSFRR368 pKa = 11.84QYY370 pKa = 11.05KK371 pKa = 10.35PEE373 pKa = 4.09TPLQDD378 pKa = 3.45LVKK381 pKa = 10.61SVATMISSASNHH393 pKa = 6.21CIINGVTMQTGTPLAIEE410 pKa = 5.36DD411 pKa = 4.34YY412 pKa = 11.36VPMAVTFVAFAKK424 pKa = 10.13SRR426 pKa = 11.84YY427 pKa = 9.52DD428 pKa = 3.62SIKK431 pKa = 10.46EE432 pKa = 3.79GMKK435 pKa = 9.35MVRR438 pKa = 11.84QRR440 pKa = 11.84GTNIADD446 pKa = 3.89PNDD449 pKa = 3.54HH450 pKa = 6.89YY451 pKa = 11.46DD452 pKa = 3.65YY453 pKa = 10.98EE454 pKa = 5.63KK455 pKa = 11.06DD456 pKa = 3.51GSDD459 pKa = 3.07VKK461 pKa = 11.14SFSNHH466 pKa = 6.48IIPMKK471 pKa = 9.99NTIKK475 pKa = 10.9SLFFPMKK482 pKa = 10.59VKK484 pKa = 10.6MNDD487 pKa = 2.91NSMLIYY493 pKa = 10.44SQGILSTVVDD503 pKa = 4.88EE504 pKa = 4.92IKK506 pKa = 9.91TLFGWDD512 pKa = 2.89VWDD515 pKa = 4.04TDD517 pKa = 3.84DD518 pKa = 6.54AVIQSLPSFYY528 pKa = 11.11KK529 pKa = 9.97MEE531 pKa = 4.99DD532 pKa = 3.35VFQVTSDD539 pKa = 4.15HH540 pKa = 6.33YY541 pKa = 10.73CLSHH545 pKa = 6.15TLSVDD550 pKa = 2.39WWLEE554 pKa = 3.65GLYY557 pKa = 10.53DD558 pKa = 3.82SYY560 pKa = 11.88HH561 pKa = 7.01DD562 pKa = 3.62MRR564 pKa = 11.84VAHH567 pKa = 6.72KK568 pKa = 10.41KK569 pKa = 10.51KK570 pKa = 10.39LDD572 pKa = 3.57EE573 pKa = 4.4EE574 pKa = 4.54EE575 pKa = 4.06SRR577 pKa = 11.84KK578 pKa = 8.37TKK580 pKa = 10.7VEE582 pKa = 3.76NALLKK587 pKa = 10.24IAEE590 pKa = 4.38VLEE593 pKa = 4.54KK594 pKa = 10.5PDD596 pKa = 3.66VSDD599 pKa = 3.56GLKK602 pKa = 10.54ALSEE606 pKa = 4.12LPIISSLLEE615 pKa = 4.11KK616 pKa = 10.47KK617 pKa = 10.48SEE619 pKa = 4.29EE620 pKa = 4.14IVTKK624 pKa = 8.01PQCRR628 pKa = 11.84DD629 pKa = 2.99TEE631 pKa = 4.24KK632 pKa = 10.9PHH634 pKa = 6.37INPYY638 pKa = 10.75ADD640 pKa = 4.6AIKK643 pKa = 9.99EE644 pKa = 3.9AQAYY648 pKa = 8.93YY649 pKa = 10.94EE650 pKa = 4.04EE651 pKa = 5.67LEE653 pKa = 4.53VVNTRR658 pKa = 11.84NLRR661 pKa = 11.84GVGDD665 pKa = 3.49YY666 pKa = 10.85LFWRR670 pKa = 11.84KK671 pKa = 9.48KK672 pKa = 10.32SNYY675 pKa = 8.75ASVWGADD682 pKa = 3.46EE683 pKa = 4.25SRR685 pKa = 11.84VVLEE689 pKa = 3.47PMARR693 pKa = 11.84KK694 pKa = 9.55FYY696 pKa = 11.01SRR698 pKa = 11.84DD699 pKa = 3.43RR700 pKa = 11.84NVSIPEE706 pKa = 4.01YY707 pKa = 9.32EE708 pKa = 4.07RR709 pKa = 11.84GMTVDD714 pKa = 4.15GFVSIKK720 pKa = 9.94WVDD723 pKa = 3.48GNITEE728 pKa = 4.7DD729 pKa = 2.89TWKK732 pKa = 10.6CLSKK736 pKa = 10.95YY737 pKa = 10.72AVVLLDD743 pKa = 3.48SSCVFDD749 pKa = 4.06AGSRR753 pKa = 11.84IMPGLKK759 pKa = 9.69KK760 pKa = 10.62ALVMDD765 pKa = 4.95ANFKK769 pKa = 10.43IVIEE773 pKa = 4.87DD774 pKa = 3.86GVAGCGKK781 pKa = 6.92TTSLLKK787 pKa = 9.87QAKK790 pKa = 8.93PDD792 pKa = 3.73SDD794 pKa = 4.45LLLAANRR801 pKa = 11.84EE802 pKa = 4.15TANDD806 pKa = 3.57AKK808 pKa = 11.0ASGVIPKK815 pKa = 10.09ALEE818 pKa = 3.76YY819 pKa = 10.19RR820 pKa = 11.84VRR822 pKa = 11.84TVDD825 pKa = 3.05SYY827 pKa = 12.31LMLRR831 pKa = 11.84TWFTAEE837 pKa = 3.69RR838 pKa = 11.84LLVDD842 pKa = 3.38EE843 pKa = 5.37CFLVHH848 pKa = 7.28AGLVYY853 pKa = 10.47AAATLARR860 pKa = 11.84VKK862 pKa = 10.46EE863 pKa = 4.43VIAFGDD869 pKa = 3.67TKK871 pKa = 10.74QIPFVSRR878 pKa = 11.84IPTVKK883 pKa = 9.94LRR885 pKa = 11.84HH886 pKa = 5.69ASVVGTLKK894 pKa = 10.55PRR896 pKa = 11.84TITYY900 pKa = 8.44RR901 pKa = 11.84CPRR904 pKa = 11.84DD905 pKa = 3.4VTAVLSEE912 pKa = 4.12KK913 pKa = 10.74FYY915 pKa = 9.14GTKK918 pKa = 10.35VKK920 pKa = 9.79TFNLVKK926 pKa = 10.61QSLEE930 pKa = 3.95LKK932 pKa = 10.49NIDD935 pKa = 3.74SSTEE939 pKa = 3.66IPVEE943 pKa = 3.97KK944 pKa = 9.58DD945 pKa = 2.83TLYY948 pKa = 10.43IAHH951 pKa = 6.15TQADD955 pKa = 3.55KK956 pKa = 10.81HH957 pKa = 7.55ALMRR961 pKa = 11.84LPGMNGIEE969 pKa = 3.97VLTTHH974 pKa = 6.78EE975 pKa = 4.37AQGKK979 pKa = 7.0TRR981 pKa = 11.84DD982 pKa = 3.52HH983 pKa = 6.77VILVRR988 pKa = 11.84LSKK991 pKa = 8.17TTNLLYY997 pKa = 10.44SGKK1000 pKa = 9.12MPDD1003 pKa = 3.7AGGSHH1008 pKa = 6.45NLVGLSRR1015 pKa = 11.84HH1016 pKa = 5.77KK1017 pKa = 10.69KK1018 pKa = 7.05SLRR1021 pKa = 11.84YY1022 pKa = 9.33FSVFADD1028 pKa = 4.69DD1029 pKa = 5.34PDD1031 pKa = 3.92DD1032 pKa = 4.48QIATGIRR1039 pKa = 11.84WSKK1042 pKa = 9.12TLDD1045 pKa = 3.57EE1046 pKa = 4.68QEE1048 pKa = 3.99LAAYY1052 pKa = 8.95RR1053 pKa = 11.84AAGG1056 pKa = 3.32

Molecular weight: 119.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O57165|O57165_9BROM Putative coat protein OS=Spinach latent virus OX=42680 PE=3 SV=1
MM1 pKa = 7.53SGNAIEE7 pKa = 6.09VNGQWYY13 pKa = 10.58SPVTNNNAPSRR24 pKa = 11.84GRR26 pKa = 11.84GRR28 pKa = 11.84GRR30 pKa = 11.84GPTARR35 pKa = 11.84SRR37 pKa = 11.84NWAQSRR43 pKa = 11.84ANAARR48 pKa = 11.84SRR50 pKa = 11.84PQTLVIGTMPTNLPTWKK67 pKa = 10.16SFPGEE72 pKa = 3.75QWHH75 pKa = 5.67VVSGFSFPDD84 pKa = 2.7RR85 pKa = 11.84WNNGNIAYY93 pKa = 10.27ASMKK97 pKa = 10.17TEE99 pKa = 3.97LGKK102 pKa = 10.43IKK104 pKa = 9.39TLHH107 pKa = 6.95EE108 pKa = 3.83ITKK111 pKa = 9.57VYY113 pKa = 10.9SVMYY117 pKa = 10.15GFICKK122 pKa = 9.71SDD124 pKa = 3.16GHH126 pKa = 8.08AGFITGFDD134 pKa = 3.93TNNPTGPVAPDD145 pKa = 3.01RR146 pKa = 11.84VKK148 pKa = 10.85VKK150 pKa = 10.68KK151 pKa = 9.96NAYY154 pKa = 8.68CAKK157 pKa = 9.84QQVFPAGTTVSEE169 pKa = 5.02LKK171 pKa = 10.63NDD173 pKa = 3.14WSFIWEE179 pKa = 4.21FDD181 pKa = 3.29AAPVVGTEE189 pKa = 3.99KK190 pKa = 10.56QVTVTQFWVSTTPLPGVKK208 pKa = 9.66PPSNFLVCEE217 pKa = 4.19DD218 pKa = 3.64

Molecular weight: 24.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2548

195

1056

509.6

57.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.633 ± 0.534

2.119 ± 0.136

6.436 ± 0.679

5.024 ± 0.376

4.199 ± 0.347

5.102 ± 0.443

2.433 ± 0.383

5.416 ± 0.538

6.476 ± 0.194

8.203 ± 0.708

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.179 ± 0.228

4.16 ± 0.555

5.102 ± 0.693

3.1 ± 0.234

5.808 ± 0.114

7.535 ± 0.305

5.495 ± 0.49

8.399 ± 0.191

1.727 ± 0.286

3.454 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski