Podoviridae sp. ctjc_2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345MZF8|A0A345MZF8_9CAUD Uncharacterized protein OS=Podoviridae sp. ctjc_2 OX=2675450 PE=4 SV=1
MM1 pKa = 7.28TDD3 pKa = 3.1KK4 pKa = 11.21EE5 pKa = 4.14FFEE8 pKa = 4.47LVKK11 pKa = 11.02EE12 pKa = 4.01NVIYY16 pKa = 10.31YY17 pKa = 10.89ANACLSIPDD26 pKa = 4.13DD27 pKa = 3.83QQLTIHH33 pKa = 7.35DD34 pKa = 3.99IDD36 pKa = 5.4IIGYY40 pKa = 9.81CEE42 pKa = 3.93TLHH45 pKa = 6.15HH46 pKa = 6.12QKK48 pKa = 10.98ALAKK52 pKa = 10.4IMVIDD57 pKa = 5.19DD58 pKa = 3.97IYY60 pKa = 11.43FEE62 pKa = 5.64LILNIDD68 pKa = 3.67EE69 pKa = 4.52NEE71 pKa = 4.45LYY73 pKa = 10.71FDD75 pKa = 3.97TYY77 pKa = 11.47KK78 pKa = 11.02KK79 pKa = 10.95LEE81 pKa = 4.67DD82 pKa = 4.01GNWRR86 pKa = 11.84LIEE89 pKa = 4.21TYY91 pKa = 10.38KK92 pKa = 10.97CC93 pKa = 3.41

Molecular weight: 11.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345MZF4|A0A345MZF4_9CAUD Single-stranded DNA-binding protein OS=Podoviridae sp. ctjc_2 OX=2675450 PE=4 SV=1
MM1 pKa = 8.31DD2 pKa = 4.81IMTKK6 pKa = 10.35VYY8 pKa = 10.08FSYY11 pKa = 10.05VQVMFFNEE19 pKa = 4.05NKK21 pKa = 9.0TPSLIKK27 pKa = 10.89VNLPKK32 pKa = 10.6HH33 pKa = 6.06KK34 pKa = 10.79NGTKK38 pKa = 10.14KK39 pKa = 10.68FYY41 pKa = 11.63GEE43 pKa = 3.8MAKK46 pKa = 10.51LKK48 pKa = 9.25NPQVEE53 pKa = 5.15GYY55 pKa = 10.84RR56 pKa = 11.84LMEE59 pKa = 3.88VSRR62 pKa = 11.84VKK64 pKa = 10.71AYY66 pKa = 10.84VDD68 pKa = 3.63LPFEE72 pKa = 3.97VLEE75 pKa = 4.42RR76 pKa = 11.84GFVNDD81 pKa = 3.56

Molecular weight: 9.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19

0

19

5539

81

1363

291.5

32.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.077 ± 1.093

1.101 ± 0.205

5.543 ± 0.287

7.294 ± 0.391

4.026 ± 0.653

5.687 ± 0.25

1.625 ± 0.187

6.427 ± 0.589

6.752 ± 0.767

8.214 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.491 ± 0.222

6.319 ± 0.268

3.52 ± 0.257

4.351 ± 0.574

3.376 ± 0.233

7.276 ± 0.501

7.24 ± 0.824

5.831 ± 0.361

1.065 ± 0.156

4.784 ± 0.447
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski