Klebsiella phage KpCHEMY26

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Humphriesvirinae; Pylasvirus; Klebsiella virus KpCHEMY26

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8R4N9|A0A5B8R4N9_9CAUD Uncharacterized protein OS=Klebsiella phage KpCHEMY26 OX=2596966 PE=4 SV=1
MM1 pKa = 7.54GLYY4 pKa = 9.98LYY6 pKa = 9.58CQNTEE11 pKa = 3.87CGSYY15 pKa = 10.49VDD17 pKa = 6.11GMDD20 pKa = 4.68CQLCGWKK27 pKa = 9.71QPPPEE32 pKa = 4.62PEE34 pKa = 3.8FEE36 pKa = 4.26LPEE39 pKa = 4.43DD40 pKa = 4.17LKK42 pKa = 11.29QAIQDD47 pKa = 3.54ALDD50 pKa = 4.0YY51 pKa = 11.02VGHH54 pKa = 7.14DD55 pKa = 4.0CRR57 pKa = 11.84LDD59 pKa = 3.43HH60 pKa = 7.51HH61 pKa = 6.49GLCQEE66 pKa = 4.81HH67 pKa = 6.71GLQEE71 pKa = 4.34GDD73 pKa = 3.62SCFAGVFRR81 pKa = 11.84KK82 pKa = 10.21YY83 pKa = 10.96LEE85 pKa = 4.01

Molecular weight: 9.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8R495|A0A5B8R495_9CAUD Acyl-CoA reductase OS=Klebsiella phage KpCHEMY26 OX=2596966 PE=4 SV=1
MM1 pKa = 6.91MRR3 pKa = 11.84KK4 pKa = 9.27SLIQGTKK11 pKa = 10.32ADD13 pKa = 3.63VARR16 pKa = 11.84MKK18 pKa = 10.56KK19 pKa = 9.83RR20 pKa = 11.84EE21 pKa = 4.03LTKK24 pKa = 10.65QVTRR28 pKa = 11.84SDD30 pKa = 3.0NCAPRR35 pKa = 11.84RR36 pKa = 11.84IVTFNHH42 pKa = 6.28PCIQEE47 pKa = 4.05VFF49 pKa = 3.52

Molecular weight: 5.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

20373

37

3629

251.5

28.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.281 ± 0.434

0.908 ± 0.207

6.15 ± 0.213

6.739 ± 0.279

3.681 ± 0.207

6.19 ± 0.184

1.581 ± 0.148

6.312 ± 0.197

6.41 ± 0.313

8.713 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.773 ± 0.146

5.497 ± 0.257

3.981 ± 0.159

4.717 ± 0.392

4.388 ± 0.177

6.165 ± 0.235

6.101 ± 0.246

6.779 ± 0.239

0.987 ± 0.106

3.647 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski