Escherichia phage pro483

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Peduovirus; Escherichia virus pro483

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F7LDQ8|A0A0F7LDQ8_9CAUD Tail protein OS=Escherichia phage pro483 OX=1649240 GN=pro483_38 PE=4 SV=1
MM1 pKa = 7.41NNAEE5 pKa = 4.68LNSEE9 pKa = 5.25LIATMAGEE17 pKa = 3.8ITVYY21 pKa = 10.79NFDD24 pKa = 3.56VMSWEE29 pKa = 4.45YY30 pKa = 10.6ISASTEE36 pKa = 3.66YY37 pKa = 10.68LAIGVGIPAYY47 pKa = 10.45SCLDD51 pKa = 3.13APGAYY56 pKa = 9.25KK57 pKa = 10.35AGYY60 pKa = 8.88AICRR64 pKa = 11.84SADD67 pKa = 3.53FNSWEE72 pKa = 4.19YY73 pKa = 11.36VPDD76 pKa = 3.41HH77 pKa = 6.93RR78 pKa = 11.84GEE80 pKa = 4.47IIYY83 pKa = 8.55STEE86 pKa = 3.76TGEE89 pKa = 4.49SKK91 pKa = 10.79EE92 pKa = 3.68ITAPGDD98 pKa = 3.5YY99 pKa = 10.32PEE101 pKa = 4.5NTTTIAPLTPYY112 pKa = 10.6DD113 pKa = 3.4KK114 pKa = 10.5WDD116 pKa = 3.49GEE118 pKa = 4.17KK119 pKa = 10.04WVTDD123 pKa = 3.46TEE125 pKa = 4.47AQHH128 pKa = 6.18SAAVDD133 pKa = 3.04AAEE136 pKa = 4.56AQRR139 pKa = 11.84QSLIDD144 pKa = 3.35AAMASISLIQLKK156 pKa = 9.88LQAGRR161 pKa = 11.84KK162 pKa = 6.48LTQAEE167 pKa = 4.49TTRR170 pKa = 11.84LNAVLDD176 pKa = 4.24YY177 pKa = 10.71IDD179 pKa = 5.46AVTATDD185 pKa = 3.41TSTAPDD191 pKa = 4.27VIWPEE196 pKa = 3.88LPEE199 pKa = 4.01AA200 pKa = 4.64

Molecular weight: 21.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F7LDU4|A0A0F7LDU4_9CAUD Terminase OS=Escherichia phage pro483 OX=1649240 GN=pro483_17 PE=4 SV=1
MM1 pKa = 7.1QVHH4 pKa = 7.34EE5 pKa = 4.37YY6 pKa = 10.21TGGAPALVALRR17 pKa = 11.84RR18 pKa = 11.84NRR20 pKa = 11.84GGKK23 pKa = 9.74RR24 pKa = 11.84MQLNAFLGTPKK35 pKa = 10.35NEE37 pKa = 3.91KK38 pKa = 9.47MLL40 pKa = 4.41

Molecular weight: 4.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

9277

40

815

215.7

23.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.046 ± 0.627

0.949 ± 0.14

5.454 ± 0.282

6.511 ± 0.264

3.298 ± 0.261

6.791 ± 0.339

1.886 ± 0.179

5.379 ± 0.171

5.53 ± 0.328

9.152 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.76 ± 0.2

3.87 ± 0.224

4.42 ± 0.299

3.902 ± 0.242

6.586 ± 0.385

6.08 ± 0.27

6.619 ± 0.341

6.511 ± 0.312

1.423 ± 0.134

2.835 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski