Geothermobacter ehrlichii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfuromonadales; Geobacteraceae; Geothermobacter

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2981 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5D3WGY7|A0A5D3WGY7_9DELT 30S ribosomal protein S10 OS=Geothermobacter ehrlichii OX=213224 GN=rpsJ PE=3 SV=1
MM1 pKa = 7.16KK2 pKa = 10.36RR3 pKa = 11.84FVRR6 pKa = 11.84FLIVLVATLSCLPPVSALAFEE27 pKa = 4.95AEE29 pKa = 4.0GDD31 pKa = 4.11VYY33 pKa = 11.42AGIYY37 pKa = 10.1DD38 pKa = 3.65KK39 pKa = 11.31YY40 pKa = 10.37LWRR43 pKa = 11.84GFDD46 pKa = 3.57LSGGLPVAQGGVDD59 pKa = 3.52VAIGGFTVSYY69 pKa = 8.64WSNMQLKK76 pKa = 9.51NDD78 pKa = 3.95AGEE81 pKa = 4.21GLPAGEE87 pKa = 4.46VNEE90 pKa = 4.3TDD92 pKa = 3.17LTLDD96 pKa = 3.49YY97 pKa = 11.15SFEE100 pKa = 4.77PGDD103 pKa = 3.77LVSVSVGNIFYY114 pKa = 10.9ALDD117 pKa = 3.78GLEE120 pKa = 4.56DD121 pKa = 3.61TSEE124 pKa = 4.31LYY126 pKa = 10.86AGVSFDD132 pKa = 4.55VIASPSLTAYY142 pKa = 10.47YY143 pKa = 10.64DD144 pKa = 3.13WDD146 pKa = 3.64KK147 pKa = 11.87AEE149 pKa = 4.19KK150 pKa = 10.04KK151 pKa = 10.77GLFFVFAVSHH161 pKa = 6.01CFEE164 pKa = 4.39PVKK167 pKa = 10.63GLSVTPGLAISWNEE181 pKa = 3.61EE182 pKa = 2.98SDD184 pKa = 4.09YY185 pKa = 11.5AIGSYY190 pKa = 10.64SDD192 pKa = 3.02WHH194 pKa = 7.28NYY196 pKa = 7.0EE197 pKa = 3.95ASLGIDD203 pKa = 3.53YY204 pKa = 10.66AITDD208 pKa = 5.07AIGISASYY216 pKa = 10.14LFSEE220 pKa = 5.64GISDD224 pKa = 3.99EE225 pKa = 3.92ARR227 pKa = 11.84QVIDD231 pKa = 3.6SEE233 pKa = 4.7SAAGLSVSLSFF244 pKa = 5.36

Molecular weight: 26.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5D3WGS3|A0A5D3WGS3_9DELT Histidine kinase OS=Geothermobacter ehrlichii OX=213224 GN=EDC39_11741 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.9QPSRR9 pKa = 11.84ISRR12 pKa = 11.84KK13 pKa = 7.55RR14 pKa = 11.84THH16 pKa = 6.52GFRR19 pKa = 11.84KK20 pKa = 10.09RR21 pKa = 11.84MQTKK25 pKa = 9.64NGRR28 pKa = 11.84AVIRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.43GRR39 pKa = 11.84KK40 pKa = 8.04VLSVTIPQKK49 pKa = 10.97

Molecular weight: 5.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2981

0

2981

958005

29

2700

321.4

35.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.757 ± 0.062

1.346 ± 0.023

5.573 ± 0.033

6.807 ± 0.056

3.901 ± 0.029

8.211 ± 0.045

2.046 ± 0.021

5.372 ± 0.039

3.819 ± 0.044

11.468 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.224 ± 0.018

2.764 ± 0.029

4.821 ± 0.025

3.496 ± 0.024

7.976 ± 0.062

4.881 ± 0.035

4.679 ± 0.04

7.237 ± 0.039

1.109 ± 0.016

2.511 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski